Analysis of ras genes mutations in human hepatic, biliary and pancreatic malignant tumors

人肝胆胰恶性肿瘤ras基因突变分析

• 批准号: 03807041
• 负责人: ITO Yoshimi
• 金额: $ 1.15万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807041/
• 关键词: ras oncogene; point mutation; P C R; hepatocellular carcinoma; bile duct cancer; gallbladder cancer; pancreatic cancer; One Point Mutation

The ras gene is one of the oncogenes most commonly detected in human cancers, and it consists of three families (H-ras, K-ras,N-ras). There genes are concerted to active oncogenes by point mutations occurring in either codon 12,13,or 61. We analyzed mutation of there codons in primary hepatic malignant tumors and panceatic cancers by a method to directly sequence nucleotides, using polymerase chain reaction and a direct sequencing method. There were no point mutations in any of hepatocellular carcinomas or hepatoblastoma around codon 12,13,or 61 ras genes. In contrast, there were point mutations in the all pancreatic cancers.

ras基因是人类癌症中最常见的癌基因之一，由三个家族（H-ras、K-ras、N-ras）组成。这些基因通过发生在密码子12、13或61处的点突变而协调成活性癌基因。我们采用聚合酶链反应和直接测序的方法，对原发性肝恶性肿瘤和胰腺癌的三种密码子进行了突变分析。在肝细胞癌和肝母细胞瘤中均未发现ras基因第12、13和61位密码子附近的点突变。相反，在所有胰腺癌中存在点突变。

项目成果

Minoru Tada,Masao Omata,Masao Ohto: "Ras gene Mutations in Iutraductal Papillary Neoplasms of the Paucreas:analysis in Five cases" CANCER. 67. 634-637 (1991)

Minoru Tada，Masao Omata，Masao Ohto：“Paucreas 导管内乳头状肿瘤中的 Ras 基因突变：五例分析”癌症。

Minoru Tada: "Ras gene mutations in intraductual papillary neoplasms of the pancreas" Cancer. 67. 634-637 (1991)

Minoru Tada：“胰腺导管内乳头状肿瘤中的 Ras 基因突变”癌症。

Minoru Tada: "Analysis of ras gene mutations in biliary and panceatic tumors by polymerase chain reaction and direct sequencing" Cancer. 66. 930-935 (1990)

Minoru Tada：“通过聚合酶链反应和直接测序分析胆道和胰腺肿瘤中的 ras 基因突变”癌症。

Miniru Tada: "High incidence of ras gene mutation in intra-hepatic cholangiocarcinoma" Cancer. 69. 1115-1118 (1992)

Miniru Tada：“肝内胆管癌中ras基因突变的高发生率”癌症。

Minoru Tada: "Clinical application of ras gene mutation for diagnosis of pancreatic adenocarcinoma" Gastroenterology. 100. 233-238 (1991)

Minoru Tada：“ras基因突变诊断胰腺腺癌的临床应用”胃肠病学。