Study on hybrid type urinary tract using autologous mucosal cells

1.人工胶原海绵与培养的自体细胞重建膀胱的实验分析：本实验选用日本大白兔12只。在全身麻醉下，取膀胱穹隆粘膜层和肌层。这些组织用酶处理，并分别在DMEM培养液中孵育。连续培养后，将细胞接种于兔胶原海绵片上，在相同条件下孵育。术后1~2周，用该胶原海绵进行膀胱重建。对照组用不含种子的兔胶原海绵。结果：1.两组间上皮细胞和肌细胞生长情况无明显差异。胶原包被聚乙醇酸片重建兔膀胱的实验研究：本实验采用胶原包被聚乙醇酸胶片。实验选用14只日本大白兔。C-PGA片术前与自体血孵育(凝血前组，第1组)，与1型寰胶原孵育(组2)。2~12周后处死动物。术后7周可见肌层再生，且1组组织再生好于2组。

1. Experimental analysis of bladder reconstruction using atelocollagen sponge and cultured autologous cells:Twelve Japanese white rabbits were examined in this study. Under general anesthesia, mucosal layer and muscular layer were taken from the dome of the urinary bladder. These tissues were treated with enzymes,and were incubated in DMEM medium, separately. After serial culture, cells were seeded on atelocollagen sponge sheet, and were incubated in the same condition. After 1-2 weeks, bladder reconstruction was performed using this atelocollagen sponge. As a control group, rabbits were treated with non-seeded atelocollagen sponge. There was no difference in growth of epithelial and muscular cells between the 2 groups.2. Experimental reconstruction of the urinary bladder using atelocollagen-coated polyglycolic acid sheet in rabbit:Atelocollagen-coated polyglycolic acid ( C-PGA ) sheet was used in this experiment. Fourteen Japanese white rabbit were used. Before operation, C-PGA sheet was incubated with autologous blood ( pre-clotting,group 1 ), and was incubated with type1 atelocollagen ( group 2). After 2-12 weeks, rabbits were sacrificed. After 7 weeks, regrowth of muscle layer was seen, and tissue regrowth was better in group 1 than group 2.