Research on carbon oxide as a neurotransmitter in the smooth muscle of the urogenital organs

1)组成型和诱导型血红素加氧酶（HOs）在泌尿生殖器官平滑肌中的表达研究。方法1：免疫组化研究；ABC法用抗大鼠HO1和HO2血清对大鼠阴茎组织低温冷冻切片进行免疫组化检测。方法2：分子生物学研究；从大鼠阴茎组织中提取总RNA，将总RNA逆转录为cDNA。随后，使用两种大鼠HO特异性引物对进行聚合酶链反应（PCR）。或H02。扩增的PCR产物在琼脂糖凝胶中电泳，并进行分级。结果：NADPH-diaphorase染色和HO-2免疫组化染色能明显染色神经样结构，HO-1染色不能。RT/PCR证实了HO-2 mRNA的表达，而HO-1 mRNA未表达。2) P2嘌呤能受体在泌尿生殖器官平滑肌中的存在及功能研究。方法…更多1：功能检查；使用电刺激对大鼠膀胱组织进行等长收缩研究，以确定非肾上腺素能、非胆碱能活性的存在，并确认拮抗剂对P2X或P2Y受体的作用。方法2：分子生物学研究；从大鼠阴茎、膀胱和精囊组织中提取总RNA，并将总RNA逆转录为cdna。随后，使用大鼠P2X或P2Y受体特异性引物对进行聚合酶链反应（POR）。放大的流行音乐。产物在琼脂糖凝胶中电泳，并进行粒度分馏。使用上述组织的细胞体切片和大鼠P2Xor P2Y受体特异性探针进行原位杂交。结果：在大鼠阴茎中。P2YI嘌呤受体mRNA表达，在大鼠精囊中，P2X2和P2YI嘌呤受体均表达。在大鼠膀胱中，P2X2和p2y1。嘌呤受体表达，p2x介导的收缩和p2x介导的舒张得到药理学证实。少

1) Research on the expression of constitutive and inducible heme-oxygenases (HOs) in the smooth muscle of the urogenital organs.Methods 1 : Immunohistochemical study ; Immunohistochemical examination of the cryostat sections of the rat penile tissue using antiserum against rat HO1 and HO2 by ABC method.Methods 2 : Molecular biological study ; Total RNA was extracted from the rat penile tissue, and the total RNA was reverse-transcribed into cDNA.Subsequently, polymerase chain reaction (PCR) was performed using primer pairs specific for either rat HO]. or H02. Amplified PCR products were electrophoreses in agarose gel, and were size-fractionated.Results : Nerve-like structures which could be stained by NADPH-diaphorase staining and HO-2 immunohistochemistry, but not by HO-1, were clerly confirmed. The expression of HO-2 mRNA, but not HO-1 mRNA was coafirmed by RT/PCR.2) Research on the presence and function of P2 purinergic receptors in the smooth muscle of the urogenital organs.Metohds … More 1 : Functional examination ; Isometric contraction studies of rat bladder tissues using electrical stimulation was performed, to determine the presence of non-adrenergic, non-cholinergic activity, and to confirm the effect of antagonists against P2X or P2Y receotor.Methods 2 : Molecular biological study ; Total RNA was extracted from the rat penile, bladder, and seminal vesicle tissues, and the total RNAs were reverse-transcribed into cDNAs. Subsequently, polymerase chain reaction (POR) was performed using primer pairs specific for either rat P2X or P2Y receptor. Amplified POP.products were electrophoreses in agarose gel, and were size-fractionated. In situ hybridization was performed using cyostat sections of above tissues and probes speciflc for either rat P2Xor P2Y receptor.Results : In the rat penis. P2YI purinoceptor mRNA was expressed, In the ratseminal vesicle, both P2X2 and P2YI purinoceptors were expressed. In the rat urinary bladder, both P2X2 and P2Y 1. purinoceptors were expressed, and P2X-mediated contraction and P2Y-mediatect relaxation were pharmacologically confirmed. Less