Research on the afferent transduction in the lower urinary tract

下尿路传入传导的研究

• 批准号: 23390381
• 负责人: TAKEDA Masayuki
• 金额: $ 12.31万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390381/
• 关键词: Afferent nerve; Urinary bladder; Overactive bladder; イオンチャネル; TRP; VNUT; PIEZO; P2Y6; ATP; Piezo1; メカノセンサー; 膀胱; 尿路上皮

We performed a systematic analysis of the molecular and functional expression of Piezo1 channels in the urothelium.Immunofluorescence examination demonstrated abundant expression of Piezo1 in the mouse and human urothelium. Urothelial cells isolated from mice exhibited a Piezo1-dependent increase in cytosolic Ca2+ concentrations in response to mechanical stretch stimuli, leading to potent ATP release; this response was suppressed in Piezo1- knockdown cells. In addition, Piezo1 and TRPV4 distinguished different intensities of mechanical stimulus.Moreover, GsMTx4, an inhibitor of stretch-activated channels, attenuated the Ca2+ influx into urothelial cells and decreased ATP release from them upon stretch stimulation. These results suggest that Piezo1 senses extension of the bladder urothelium, leading to production of an ATP signal. Thus, inhibition of Piezo1 might provide a promising means of treating bladder dysfunction.

我们对Piezo 1通道在尿路上皮中的分子和功能表达进行了系统的分析，免疫荧光检测显示Piezo 1在小鼠和人尿路上皮中有丰富的表达。从小鼠分离的尿路上皮细胞表现出Piezo 1依赖性增加胞浆Ca 2+浓度响应机械拉伸刺激，导致有效的ATP释放，这种反应被抑制在Piezo 1敲低细胞。此外，Piezo 1和TRPV 4还能区分不同强度的机械刺激，而牵张激活通道抑制剂GsMTx 4能抑制牵张刺激时细胞内Ca ~（2+）内流和ATP释放。这些结果表明，Piezo 1感觉膀胱尿道扩张，导致ATP信号的产生。因此，抑制Piezo 1可能提供治疗膀胱功能障碍的有希望的手段。

项目成果

Intraoperative fluoroscopic monitoring during TVM surgery: safer procedure even for beginners.

TVM 手术期间的术中荧光镜监测：即使对于初学者来说也是更安全的手术。

• DOI: 10.4236/oju.2012.22012
• 发表时间: 2012
• 期刊: Open Journal of Urology
• 作者: Kobayashi H;Sawada N;Kira S;Miyamoto T;Haneda Y;Zakoji H;Tsuchida T;Araki I;Takeda M
• 通讯作者: Takeda M

Decreased Expression of the Epithelial Ca2+ Channel TRPV5 and TRPV6 Human Cell Darinoma Associated With Vitamin D Receptor

与维生素 D 受体相关的上皮 Ca2 通道 TRPV5 和 TRPV6 人细胞 Darinoma 表达减少

• 发表时间: 2011
• 期刊: JOURNAL OF UROLOGY
• 影响因子: 6.6
• 作者: Shin M;Mizokami A;Kim J;Ofude M;Konaka H;Kadono Y;Kitagawa Y;Miwa S;Kumaki M;Keller ET;Namiki M;Wu Yong-yang
• 通讯作者: Wu Yong-yang

Connexin43 hemichannel-mediated regulation of connexin43.

Connexin43 半通道介导的 connexin43 调节。

• DOI: 10.1371/journal.pone.0058057
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Li K;Chi Y;Gao K;Yan Q;Matsue H;Takeda M;Kitamura M;Yao J
• 通讯作者: Yao J

Clinical Efficacy and Safety of Imidafenacin as Add-on Treatment for Persistent Overactive Bladder Symptoms Despite -Blocker Treatment in Patients with BPH:

伊米达那新作为辅助治疗持续性膀胱过度活动症症状的临床疗效和安全性

• 发表时间: 2013
• 期刊: Urology
• 影响因子: 2.1
• 作者: Masayuki Takeda;Osamu Nishizawa;Momokazu Gotoh,
• 通讯作者: Momokazu Gotoh,

NfKAPPAB/INOS/CAMP SIGNALING CASCADE MEDIATES INFLAMMATORY CYTOKINES-INDUCED UPREGULATION OF CONNEXIN43 EXPRESSION AND FUNCTION IN UEINARY TRACT INFECTION

NfKAPPAB/INOS/CAMP 信号级联介导炎症细胞因子诱导的泌尿道感染中 CONNEXIN43 表达和功能的上调

• 发表时间: 2011
• 作者: Yokoyama O;Hiro S;Hotta S;Mogami S;Yamagami H;Kai Li
• 通讯作者: Kai Li