Identification of differential regulator of mitosis and meiosis

有丝分裂和减数分裂差异调节因子的鉴定

• 批准号: 03833006
• 负责人: IINO Yuichi
• 金额: $ 1.22万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03833006/
• 关键词: fission yeast; S. pombe; mitosis; meiosis; cdc gene; cell cycle; cyclin; ribonucleotide reductase; schizosaccharomyces pombe; Schizosaccharomyces pombe; rivonucleotide reductase

Involvement of the cdc2, cdc13 and cdc25 genes in meiosis in Schizosaccharomyces pombe was investigated. Using temperature- sensitive mutants and three different conditions to induce meiosis, it was found that, in contrast to the previous reports, all of these genes are required for both meiosis I and meiosis II. The cdc2 gene, which is known to be essential for the initiation of both M-phase and S-phase in mitosis, is also required for premeiotic DNA synthesis.Expression of the cdc25 and cdc13 genes was induced in concomitant with premeiotic DNA synthesis, reaching a sharp peak before the first nuclear division. Expression of cdc22 was also induced but with a peak before premeiotic DNA synthesis. The induction of cdc25 and cdc13 was hampered if DNA synthesis was inhibited, but that of cdc22 was not. The mei4 gene that is essential for meiosis is required for the induction of cdc13 and cdc25. Dependency on DNA synthesis of the induction of genes that are important for activation of p34^<cdc2> kinase has not been observed in mitosis and appears to be unique to meiosis. This may constitute a mechanism of checkpoint control in meiosis.

研究了cdc2、cdc13和cdc25基因在pombe Schizosaccharomyces减数分裂中的作用。利用温度敏感突变体和三种不同的条件诱导减数分裂，发现与以往报道不同，所有这些基因都是减数分裂I和减数分裂II所必需的。cdc2基因是有丝分裂中m期和s期起始所必需的，也是减数分裂前DNA合成所必需的。cdc25和cdc13基因的表达与减数分裂前DNA合成同时被诱导，在第一次核分裂前达到高峰。cdc22的表达也被诱导，但在减数分裂前DNA合成前达到峰值。如果DNA合成受到抑制，则cdc25和cdc13的诱导受到阻碍，而cdc22的诱导不受影响。对于减数分裂至关重要的mei4基因是诱导cdc13和cdc25所必需的。对p34^<cdc2>激酶活化重要的基因的诱导依赖DNA合成在有丝分裂中尚未观察到，似乎是减数分裂所特有的。这可能是减数分裂中检查点控制的一种机制。

项目成果

A.SUGIMOTO: "Schizosaccharomyces pombe stell^+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development" Genes and Development. 5. 1990-1999 (1991)

A.SUGIMOTO：“粟酒裂殖酵母编码具有 HMG 基序的转录因子，该基序是性发育的关键调节因子”《基因与发育》。

J.-F.Brunet: "Identification of a peptide specific for Aplysia sensory neurons by PCR-based differential screening" Science. 252. 856-859 (1991)

J.-F.Brunet：“通过基于 PCR 的差异筛选鉴定海兔感觉神经元特异性肽”《科学》。

J.-F. Brunet: "Identification of a peptide specific for Aplysia sensory neurons by PCR-based differential screening" Science. 252. 856-859 (1991)

J.-F。

J.ーF.Brunet: "Identification of a peptide specific for Aplysia sensory neurons by PCRーbased differential screening." Science. 252. 856-859 (1991)

J.-F.Brunet：“通过基于 PCR 的差异筛选鉴定海兔感觉神经元特异性肽。” 252. 856-859 (1991)。

Y. Iino: "S.pombe pacl^+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase" EMBO Journal. 10. 221-226 (1991)

Y. Iino：“S.pombe pacl^，其过度表达抑制性发育，编码核糖核酸酶 III 样 RNase”EMBO 杂志。