Study of the functions of the Ras-MAPK pathway and the insulin pathway in neural plasticity of C. elegans

Ras-MAPK通路和胰岛素通路在秀丽隐杆线虫神经可塑性中的作用研究

基本信息

  • 批准号:
    15370071
  • 负责人:
  • 金额:
    $ 9.6万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

1)We reported before that the Ras-MAPK pathway acts in the olfactory neurons in C.elegans and is required for efficient odor responses (Hirotsu et al., 2000). In this study, we found that the Ras-MAPK pathway is also involved in olfactory adaptation and conducted detailed analyses on this role. We found that this form of olfactory adaptation, which we call "early adaptation", depends not only on sensory neurons, but also on a class of interneurons, AIY. AIY is a class of interneurons that receive synaptic inputs from sensory neurons including olfactory neurons AWA and AWC. Several mutants of the Ras-MAPK pathway showed defects in early adaptation. Moreover, we showed that Ras acts in AIY neurons, because expression of let-60 rasgene in AIY neurons rescues the defect of the let-60 mutants in early adaptation. By immunohistochemistry, we observed that MAPK is activated in olfactory neurons and in AIY interneurons by odor stimulation.2)We have previously established an assay system for associative learning. C.elegansis usually attracted to NaCl. However, after experiencing NaCl in the absence of food, they learn to avoid NaCl. Mutants for insulin INS-1, insulin receptor, and the downstream factors PI-3 kinase, PDK kinase and Akt kinase showed defects in associative learning. Through cell-specific expression experiments, we found that INS-1 acts in the interneuron AIA, and PI 3-kinase acts in ASE neurons, a pair of sensory neurons that sense NaCl. These results suggest that AIA neurons, which receive synaptic inputs from sensory neurons, send a retrograde signal via INS-1 to sensory neurons. ASE neurons consist of a bilaterally symmetrical pair of neurons. Of these, PI-3-kinase acts only in the right member.
1)我们之前报道了Ras-MAPK通路在秀丽隐杆线虫的嗅觉神经元中起作用,并且是有效气味反应所需的(Hirotsu et al.,2000年)。本研究发现Ras-MAPK通路也参与嗅觉适应,并对其作用进行了详细的分析。我们发现,这种形式的嗅觉适应,我们称之为“早期适应”,不仅依赖于感觉神经元,还依赖于一类中间神经元,AIY。AIY是一类中间神经元,其从包括嗅觉神经元AWA和AWC的感觉神经元接收突触输入。Ras-MAPK途径的几个突变体在早期适应中表现出缺陷。此外,我们发现Ras在AIY神经元中起作用,因为AIY神经元中let-60 ras基因的表达挽救了let-60突变体在早期适应中的缺陷。通过免疫组织化学方法,我们观察到气味刺激可激活嗅觉神经元和AIY中间神经元中的MAPK。2)我们建立了一个用于联想学习的检测系统。C. elegansis通常吸引NaCl。然而,在没有食物的情况下经历了NaCl之后,他们学会了避免NaCl。胰岛素INS-1、胰岛素受体和下游因子PI-3激酶、PDK激酶和Akt激酶的突变体在联想学习中表现出缺陷。通过细胞特异性表达实验,我们发现INS-1作用于中间神经元AIA,PI 3-激酶作用于ASE神经元,这是一对感知NaCl的感觉神经元。这些结果表明,从感觉神经元接收突触输入的AIA神经元通过INS-1向感觉神经元发送逆行信号。ASE神经元由两侧对称的一对神经元组成。其中,PI-3-激酶仅在右侧成员中起作用。

项目成果

期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails
使用 Poly(A) 尾的靶向下拉鉴定秀丽隐杆线虫中纤毛感觉神经元表达的基因
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kunitomo;H.;Uesugi;H.;Kohara;Y.;Iino;Y.
  • 通讯作者:
    Y.
Hanazawa, Kawasaki, Kunitomo, Gengyo-Ando, Bennett, Mitani, Iino: "The Caenorhabditis elegans eukaryotic initiation factor 5A homologue, IFF-1, is required for germ cell proliferation, gametogenesis and localization of the P-granule component PGL-1"Mechan
Hanazawa、Kawasaki、Kunitomo、Gengyo-Ando、Bennett、Mitani、Iino:“秀丽隐杆线虫真核起始因子 5A 同源物 IFF-1 是生殖细胞增殖、配子发生和 P 颗粒成分 PGL-1 定位所必需的”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Identification of ciliated sensory neuron-expressed genes in Caenorhabdltls elegans using targeted pull-down of poly(A) tails
使用 Poly(A) 尾的靶向下拉鉴定秀丽隐杆线虫中纤毛感觉神经元表达的基因
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kunitomo;H.;Uesugi;H.;Kohara;Y.;Iino;Y.
  • 通讯作者:
    Y.
Neutral circuit-dependent odor adaptation in C.elegans is regulated by the Ras-MAPK pathway
线虫中中性回路依赖性气味适应受 Ras-MAPK 通路调节
Neural circuit-dependent odor adaptation in C-elegans is regulated by the Ras-MAPK pathway
  • DOI:
    10.1111/j.1365-2443.2005.00856.x
  • 发表时间:
    2005-06-01
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    Hirotsu, T;Iino, Y
  • 通讯作者:
    Iino, Y
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IINO Yuichi其他文献

Identification of genes involved in the pheromone signaling that regulates olfactory plasticity
鉴定参与调节嗅觉可塑性的信息素信号传导的基因
  • DOI:
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TORIYABE Hiroshi;YAMADA Koji;SAWAMURA Yoshiyuki;IINO Yuichi
  • 通讯作者:
    IINO Yuichi
EFFECT OF INITIAL IMPERFECTION ON LOCAL BUCKLING BEHAVIOR OF SQUARE HOLLOW SECTION MEMBER
初始缺陷对方形空心截面构件局部屈曲行为的影响

IINO Yuichi的其他文献

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{{ truncateString('IINO Yuichi', 18)}}的其他基金

A comprehensive study of the molecular mechanisms of associative learning by a behavioral genetic approach.
通过行为遗传学方法全面研究联想学习的分子机制。
  • 批准号:
    21370002
  • 财政年份:
    2009
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional study of the Ras-MAP kinase pathway in the nervous system
神经系统 Ras-MAP 激酶通路的功能研究
  • 批准号:
    13480224
  • 财政年份:
    2001
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Elucidation of The Molecular Mechanims of Chemotaxis and Its Plasticity in the Nematode Caenorhabditis elegans
秀丽隐杆线虫趋化性及其可塑性分子机制的阐明
  • 批准号:
    11680672
  • 财政年份:
    1999
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of estrogen-induced genes in breast cancer
乳腺癌雌激素诱导基因分析
  • 批准号:
    06454359
  • 财政年份:
    1995
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Systematic Search for the Genes that Program Sex Behavior in Caenorhabditis elegans
系统性搜索秀丽隐杆线虫性行为基因
  • 批准号:
    07808093
  • 财政年份:
    1995
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of differential regulator of mitosis and meiosis
有丝分裂和减数分裂差异调节因子的鉴定
  • 批准号:
    03833006
  • 财政年份:
    1991
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Slo2 channels in Olfactory Adaptation
嗅觉适应中的 Slo2 通道
  • 批准号:
    10823705
  • 财政年份:
    2023
  • 资助金额:
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考虑嗅觉适应的气味刺激对人类疼痛的影响
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Development of energy saving method using dynamic controlling of ventilation rate based on olfactory adaptation
基于嗅觉适应的通风率动态控制节能方法的开发
  • 批准号:
    24656336
  • 财政年份:
    2012
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    $ 9.6万
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    Grant-in-Aid for Challenging Exploratory Research
C. elegans olfactory adaptation
C. 线虫嗅觉适应
  • 批准号:
    7845122
  • 财政年份:
    2009
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    $ 9.6万
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Towards an understanding of olfactory adaptation
了解嗅觉适应
  • 批准号:
    7112920
  • 财政年份:
    2004
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    $ 9.6万
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Towards an understanding of olfactory adaptation
了解嗅觉适应
  • 批准号:
    6943854
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C. elegans olfactory adaptation
C. 线虫嗅觉适应
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    2004
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C. elegans olfactory adaptation
C. 线虫嗅觉适应
  • 批准号:
    7314124
  • 财政年份:
    2004
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C. elegans olfactory adaptation
C. 线虫嗅觉适应
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Olfactory Adaptation in C. Elegans
线虫的嗅觉适应
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