Systematic Search for the Genes that Program Sex Behavior in Caenorhabditis elegans
系统性搜索秀丽隐杆线虫性行为基因
基本信息
- 批准号:07808093
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have undertaken to isolate mutants defective in sex behavior in the nematode C.elegans. The him-5 mutant of C.elegans gives rise to high frequency of males in its offsprings. The plg-1 mutant males deposit gelatinous material (called plug) around the vulva of hermaphrodites after sperm transfer. Double mutant him-5 plg-1 was treated with mutagen and mating-deficient mutants were isolated as those that fail to deposit plug. We isolated 39 such mutants. The mating behavior of C.elegans males consists of four steps : "response to contact", "turning", "vulva location" and "spicule insertion". Among the mutants isolated, 14 had defect in response to contact, 23 had defect in turning, and 5 had defect in vulva location. Some of them had defects in multiple steps. Twenty of the mutants had morphological abnormalities in the tail, which is important for the male mating behavior. Male tail has 9 pairs of sensory organs called rays. The abnormalities observed in the mutants include fusion of rays and deletion of rays. Six mutants seemed to have defects in extension and/or retraction of their spicules, which are important for sperm transfer. Abnormalities in the sensory neurons were revealed by staining with fluorescent dye DiO in 11 of the mutants without gross morphological defects. The mutants isolated in this study will be useful in elucidating the genetic control of the development of male-specific structure and function.
我们已经开始分离秀丽隐杆线虫性行为有缺陷的突变体。秀丽隐杆线虫的him-5突变体在其后代中产生高频率的雄性。在精子转移后,plg-1突变的雄性在雌雄同体的外阴周围沉积凝胶状物质(称为塞)。用诱变剂处理双突变体his -5 plg-1,分离出交配缺陷突变体,即不结塞突变体。我们分离出39个这样的突变体。秀丽隐杆线虫雄性的交配行为包括“接触反应”、“转身”、“外阴定位”和“针尖插入”四个步骤。分离的突变体中,接触反应缺陷14个,转动缺陷23个,外阴定位缺陷5个。其中一些在多个步骤中存在缺陷。其中20个突变体在尾部出现形态异常,这对雄性交配行为很重要。雄鱼的尾巴有9对感觉器官,叫做鳐鱼。在突变体中观察到的异常包括射线融合和射线缺失。6个突变体似乎在其针状体的伸展和/或收缩方面存在缺陷,这对精子转移很重要。在11个无明显形态缺陷的突变体中,用荧光染料DiO染色发现感觉神经元异常。本研究中分离的突变体将有助于阐明雄性特异性结构和功能发育的遗传控制。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
J.Kanoh: "″Schizosaccharomyces pombe gad7^+ encodes a phosphoprotein with a bZIP domain,which is required for proper G1 arrest and gene expression under nitrogen starvation″" Genes to Cells. (発表予定).
J. Kanoh:““粟酒裂殖酵母 gad7^+ 编码具有 bZIP 结构域的磷蛋白,这是在氮饥饿下正确 G1 停滞和基因表达所必需的””Genes to Cells。(即将出版)。
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- 影响因子:0
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- 通讯作者:
Y.Iino: "The role of cdc2 and other genes in meiosis in Schizosaccharomyces pombe." Genetics. 140. 1235-1245 (1995)
Y.Iino:“cdc2 和其他基因在粟酒裂殖酵母减数分裂中的作用。”
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IINO Yuichi其他文献
Identification of genes involved in the pheromone signaling that regulates olfactory plasticity
鉴定参与调节嗅觉可塑性的信息素信号传导的基因
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
TORIYABE Hiroshi;YAMADA Koji;SAWAMURA Yoshiyuki;IINO Yuichi - 通讯作者:
IINO Yuichi
EFFECT OF INITIAL IMPERFECTION ON LOCAL BUCKLING BEHAVIOR OF SQUARE HOLLOW SECTION MEMBER
初始缺陷对方形空心截面构件局部屈曲行为的影响
- DOI:
10.3130/aijs.81.893 - 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
TORIYABE Hiroshi;YAMADA Koji;SAWAMURA Yoshiyuki;IINO Yuichi;佐藤公亮,五十嵐規矩夫 - 通讯作者:
佐藤公亮,五十嵐規矩夫
IINO Yuichi的其他文献
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{{ truncateString('IINO Yuichi', 18)}}的其他基金
A comprehensive study of the molecular mechanisms of associative learning by a behavioral genetic approach.
通过行为遗传学方法全面研究联想学习的分子机制。
- 批准号:
21370002 - 财政年份:2009
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study of the functions of the Ras-MAPK pathway and the insulin pathway in neural plasticity of C. elegans
Ras-MAPK通路和胰岛素通路在秀丽隐杆线虫神经可塑性中的作用研究
- 批准号:
15370071 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional study of the Ras-MAP kinase pathway in the nervous system
神经系统 Ras-MAP 激酶通路的功能研究
- 批准号:
13480224 - 财政年份:2001
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of The Molecular Mechanims of Chemotaxis and Its Plasticity in the Nematode Caenorhabditis elegans
秀丽隐杆线虫趋化性及其可塑性分子机制的阐明
- 批准号:
11680672 - 财政年份:1999
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of estrogen-induced genes in breast cancer
乳腺癌雌激素诱导基因分析
- 批准号:
06454359 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Identification of differential regulator of mitosis and meiosis
有丝分裂和减数分裂差异调节因子的鉴定
- 批准号:
03833006 - 财政年份:1991
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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犬钩虫中Caenorhabditis elegans daf同源基因的鉴定和功能研究
- 批准号:30972181
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- 资助金额:30.0 万元
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Perception of Dead Conspecifics modulates neural signaling and lifespan in Caenorhabditis elegans
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Continuing Grant
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Decrypting nociceptive signaling pathways in Caenorhabditis elegans using behavioral analysis and mass spectrometry-based proteomics
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