Observation of lattice defect creation in protein crystals with laser scattering tomography

用激光散射断层扫描观察蛋白质晶体中晶格缺陷的产生

• 批准号: 04640343
• 负责人: SATO Kiyotaka
• 金额: $ 1.34万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640343/
• 关键词: light scattering tomography; surface microtopography; Hen-egg white lysozyme crystal; etch pit; lattice defect; crystal growth; point defect; line defect; 表面マイクロトポグラフ法

Laser scattering tomography (LST) has been applied to observe lattice defects in protein crystals for the first time. Hen-egg white lysozyme crystal of orthorhombic modification was grown by a batch method using heterogeneous crystallization method. The crystal sized employed were 1-2 mm in thickness and 5-7 mm in length. The following conclusions were obtained : (1) LST figures were observed from the crystal portions where no defect was detectable with optical microscopy. (2) The polarization dependence proved that both bulk-type and point defect-type defects are present. The bulk-type LST patterns were often parallel to the crystal edges, and assumed to correspond to inclusions. (3) The polarization dependence could differentiate the point-defect type and bulk defect type. From these results, we conclude that LST is effective to macroscopically characterize the protein crystals.

首次将激光散射层析成像技术（LST）用于观察蛋白质晶体中的晶格缺陷。采用非均相结晶法，分批培养了正交改性的蛋清溶菌酶晶体。晶体的厚度为1-2毫米，长度为5-7毫米。得到以下结论：(1)在光学显微镜未检测到缺陷的晶体部分观察到LST图。(2)极化依赖性证明了材料中存在体型和点缺陷型缺陷。体型LST模式通常与晶体边缘平行，并被认为与包裹体相对应。(3)极化依赖性可以区分点状缺陷类型和块状缺陷类型。从这些结果，我们得出结论，LST是有效的宏观表征蛋白质晶体。

项目成果

T.Koyano et al.: "Phase behavior of mixed systems of SOS and OSO" J.Physical Chemistry. 96. 10514-10520 (1992)

T.Koyano 等人：“SOS 和 OSO 混合系统的相行为”J.Physical Chemistry。

佐藤清隆: "タンパク質の結晶成長" 応用物理学会 有機分子・バイオエレクトロニクス分科会会誌. 5. 17-22 (1994)

Kiyotaka Sato：“蛋白质晶体生长”日本应用物理学会有机分子和生物电子学小组委员会杂志 5. 17-22 (1994)。

T. Koyano et al: "Phase Behavior of Mixed Systems of SOS and OSO" J. Phys. Chem.96. 10514-10520 (1992)

T. Koyano 等人：“SOS 和 OSO 混合系统的相行为”J. Phys。

T.Kayano et al.: "Phase behavior of mixed systems of SOS and OSO" J.Phys.Chem.96. 10514-10520 (1992)

T.Kayano 等人：“SOS 和 OSO 混合系统的相行为”J.Phys.Chem.96。

J.Yano et al.: "FT-IR study of polymorphie transformations in SOS POP and POS" J.Phys.Chem.97. 12967-12973 (1993)

J.Yano 等人：“SOS POP 和 POS 中多态转化的 FT-IR 研究”J.Phys.Chem.97。