权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular analyzes of genetic variation produced by somatic cell hybridization for the use of plant breeding

用于植物育种的体细胞杂交产生的遗传变异的分子分析

基本信息

批准号：
04660002
负责人：
NIIZEKI Minoru
金额：
$ 1.34万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660002/
关键词：
Oryza sativa L.Glycine max Medicago sativa Lotus corniculatus Somatic cell hybrid Nuclear DNA Mitochondrial DNA Chloroplast DNA oryza sativa イネマメ科植物細胞融合科間雑種属間雑種サザンブロット

项目摘要

Interfamilial and intergeneric somatic cell hybridization among a gramineous species (Oryza sativa L., strain A-58) and three leguminous species soybean (Glycine max (L.) Merr., cv.Harosoy), alfalfa (Medicago sativa L., cv.Dupuit) and birdsfoot trefoil (Lotus corniculatus L., cv.Viking) were examined. Both fragments of parent in some cases and altered mtDNAs were observed in the restriction profiles and Southern blots of 5 combinations of somatic hybrid calli. On the other hand, nuclear DNAs and cpDNAs were the same as those of one parent and they were identical with those of soybean, alfalfa or birdsfoot trefoil, for the banding patterns of restriction profiles and Southern blots in somatic hybrid calli. Therefore, it is assumed that some kinds of interaction between nucleus and cytoplasmic organelle and selective disadvantage for rice nucleus promote such a uniparental segregation of cpDNA.So far, it has been indicated that the organization of mitochondrial genome is correlative with morphological and physiological functions of plants. In this study, regenerated hybrid plants of soybean and birdsfoot trefoil, and of rice and birdsfoot trefoil, showed the morphological and physiologically abnormality. Actually, some regenerants from the hybrid calli of rice and birdfoot trefoil were tolerant of low temperature and low sunlight intensity in green house at wintertime. Thus, it is suggested that these regenerated hybrid plants presumably have the altered mtDNAs, as well as hybrids calli analyzed in this study.

禾本科植物（Oryza sativa L.，菌株A-58）和3种豆科大豆（Glycine max（L.）嗯，cv.Harosoy），苜蓿（Medicagosativa L.，cv.Dupuit）和百脉根三叶草（百脉根L.，cv.维京海盗）进行了检测。在5个体细胞杂种愈伤组织组合的限制性内切酶图谱和Southern杂交中，均观察到部分亲本片段和线粒体DNA的改变。体细胞杂种愈伤组织中的核DNA和cpDNA与亲本相同，与大豆、苜蓿和百脉根的核DNA和cpDNA在限制性内切酶谱和Southern杂交中的带型相同。因此，推测核质细胞器之间的某种相互作用以及水稻细胞核的选择性劣势促进了cpDNA的这种单亲分离，目前的研究表明线粒体基因组的结构与植物的形态和生理功能密切相关。在本研究中，大豆和百脉根，水稻和百脉根的再生杂种植株表现出形态和生理异常。实际上，从水稻和百脉根的杂种愈伤组织中获得的一些再生体在冬季的绿色温室中对低温和低光照强度是耐受的。因此，这些再生的杂种植株可能具有改变的mtDNA，以及本研究中分析的杂种愈伤组织。