Genetic analysis of Bradyrhizobium japonicum isolates possessing highly reiteratied DNA sequences

具有高度重复 DNA 序列的日本慢生根瘤菌分离株的遗传分析

• 批准号: 04660066
• 负责人: MINAMISAWA Kiwamu
• 金额: $ 1.34万
• 依托单位: IBARKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660066/
• 关键词: soybean; Bradyrhizobium japonicum; repeated sequence; insertion element; highly reiterated DNA sequence

During DNA fingerprinting of indigenous Bradyrhizobium japonicum by repeated sequence (RSalpha and RSbeta), we found many isolates possessing highly reiterated RSs (HRS isolates) from two fields in Japan. Some HRS isolates harbors approximately 200 copies of RSalpha on their genomes. Analysis of pulse field gel electrophoresis indicates that RSalpha copies are distributed to 74% region on HRS isolate genomes and to 17% region on normal isolate genomes. With respect to symbiotic features of HRS isolates, nodulation, nitrogenase activity and hydrogenase activity of HRS isolates were similar to that of normal isolates. However, HRS and normal isolates differed in growth rates in YM medium. Generation time of HRS isolates showed 5-9 h, While normal isolates showed 4-6 h of genaration time. These results strongly suggest that RSs transposition and genomic rearrangement occur on the genomes of B.japonicum HRS isolates.

在对日本慢生根瘤菌（Bradyrhizobium japonicum）进行重复序列（RSalpha和RSbeta）的DNA指纹图谱分析时，我们发现了来自日本两个地区的许多具有高度重复序列的菌株（HRS菌株）。一些HRS分离株在其基因组上含有约200个RSalpha拷贝。脉冲场凝胶电泳分析表明，RSalpha拷贝分布在HRS分离物基因组的74%区域和正常分离物基因组的17%区域。在共生特性方面，HRS菌株的固氮酶、氢化酶和固氮酶活性与正常菌株相似。然而，HRS和正常菌株在YM培养基中的生长速率不同。HRS菌株的世代时间为5-9 h，而正常菌株的世代时间为4-6 h。这些结果表明，日本B.japonicum HRS分离株的基因组发生了RSs转座和基因组重排。

项目成果

K.Minamisawa: "Phylogeny and ecology of Bradyrhizobium japonicum" Soil Microorganisms. 40. 37-46 (1992)

K.Minamisawa：“日本慢生根瘤菌的系统发育和生态学”土壤微生物。

K.Minamisawa, H.Iino, N.Saito, S.Onodera, Y.Nakatsuka, S.Nakazima, M.Kubota, T.Asami: "Determination of genotype group of Bradyrhizobium japonicum by indole-3-acetic acid production" Jpn.J.Soil Sci.Plant Nutr.63. 459-462 (1992)

K.Minamisawa、H.Iino、N.Saito、S.Onodera、Y.Nakatsuka、S.Nakazima、M.Kubota、T.Asami：“通过吲哚-3-乙酸生产测定日本慢生根瘤菌的基因型组”Jpn

Kiwamu Minamisawa: "Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics" Appl.Environ.Microbiol.58. 2832-2839 (1992)

Kiwamu Minamisawa：“通过重复序列和各种其他特征揭示的日本慢生根瘤菌田间分离株的遗传相关性”Appl.Environ.Microbiol.58。

K.Minamisawa et al.: "New horizons in nitrogen fixation" Kluwer Academic Publishers, 788 (1993)

K.Minamisawa 等人：“固氮的新视野”Kluwer 学术出版社，788 (1993)

Kiwamu Minamisawa: "Genetic relatodness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics." Applied and Environmental Microbiology. 58. 2832-2839 (1992)

Kiwamu Minamisawa：“重复序列和各种其他特征揭示了日本慢生根瘤菌田间分离株的遗传相关性。”