Study on relationships of fast growth and glutathione in Bradyrhizobium japonicum mutant

blr 7984是大豆根瘤菌TetR的一个阻遏子，在该基因的突变体中，细胞生长速度比野生型快。在对数生长期细胞的微阵列分析显示，相邻基因bll 7981 -3、有效呼吸所需的cbb-细胞色素氧化酶、鞭毛合成蛋白和糖转运蛋白的基因表达大幅增加，表明这些基因参与了细胞生长速度的调节。在用blr 7984破坏剂感染的大豆根瘤中的类杆菌的微阵列分析中，与感染野生型细胞的类杆菌相比，仅调节基因bll 7981 -3的表达增加，表明这三个基因是blr 7984的靶基因。尤其是编码谷胱甘肽转移酶的bll 7983被诱导得最多。为了确认bll 7983的大量诱导是否导致快速生长，产生B。开始用BL 17983过表达日本血吸虫。

blr7984 is a putative TetR repressor in Bradirhizobium japonicum and in the disuruptant of this gene, cell growth speed was faster than wild-type. Microarray analysis with the cells grown at log-phase showed a large increase in gene expression of the adjacent genes bll7981-3, cbb-cytochrome oxidases needed for efficient respiration, proteins for flagella synthesis and transporters for sugars, suggesting the involvement of these genes in regulation of cell growth speed. In microarray analysis with bacteroids from soybean nodules infected with blr7984 disruptant, expression of the adjuscent genes bll7981-3 only were increased compared with bacteroids infected with wild-type cells, indicating that these three genes are the targets of blr7984. Especially bll7983 encoding glutathione transferase was induced the most. To confirm if the large induction of bll7983 caused the fast growth or not, generation of B. japonicum overexpressed with bll7983 was started.