Global systems of symbiotic nitrogen-fixing bradyrhizobia by comparative genomics of phylogenetic super-neighbors

通过细菌系统发育超邻居的比较基因组学，对全球共生固氮慢生根瘤菌系统进行了研究。土壤慢生根瘤菌S23321与B无共生岛，与B基因组高度共线性。japonicum USDA 110的基因组序列，推测为共生慢生根瘤菌的祖先型基因组。另一方面，基因组分析表明，土壤中的寡养农单胞菌（寡养慢生根瘤菌）是一种固氮共生体的麒麟菜。事实上，A的所有文本菌株。寡养型能使A.与共生固氮的籼稻。此外，还对B.日本根瘤菌的III型蛋白分泌系统（T3SS）获得了诱导Rj2大豆的能力，表明通过T3SS分泌的效应物触发了这两个伴侣之间的不相容性。

Global systems of symbiotic nitrogen-fixing bradyrhizobia were conducted by comparative genomics of phylogenetic super-neighbors of the bacteria. Soil-dwelling Bradyrhizobium sp. strain S23321 lacked symbiosis island with high genomic co-linearity with B. japonicum USDA110, suggesting an ancestral-type genome of symbiotic bradyrhizobia. On the other hand, the genome analysis suggested soil-dwelling Agromonas oligotrophica (Bradyrhizobium oligotrophicum) is a nitrogen-fixing symbiont of Aechynomeny indica. Indeed, all texted strains of A. oligotrophica are able to nodulate A. indica with symbiotic nitrogen fixation. In addition, the rhcJ and ttsI mutants of B. japonicum for the type III protein secretion system (T3SS) gained the ability to nodulate Rj2 soybean, suggesting the effectors secreted via T3SS trigger incompatibility between these two partners.