Studies on Boron Function and Distribution in Higher Plant Cells

硼在高等植物细胞中的功能和分布研究

• 批准号: 04660069
• 负责人: MATOH Toru
• 金额: $ 1.28万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660069/
• 关键词: Boron; Boric acid; Pectic substances; Rhammnogalacturonan; Radish; 細胞壁; ニジトロナフタレンジオール; タバコ; 培養細胞

Crude cell walls were prepared from radish roots with grading, ethanol- and methanol/chloroform-washing and freeze-drying. The boron-containing polysaccharide was solubilized and purified from the crude radish cell walls by pectinase digestion and subsequent column chromatography on a DEAE-Toyopearl, Superdex-75 and DEAE-Sephacel. The purified polysaccharide had a molecular weight of 7,000 and comprised of uronic acid 52%, abarinose 17.4%, galactose 4.9% and rhammnose 9.8%, suggesting the boron-containing polysaccharide was a Rhammnogalacturonan. One molecule of the polysaccharide contained one molecule of boron as boric acid, as revealed with 11B NMR.Recovery of boron in this polysaccharide amounted to 80% of the boron of the cell walls, indicating that boron occurs in cell walls as a bound form almost exclusively with this polysaccharide. I speculate that boron may fulfill its function through binding with this polysaccharide.

从萝卜根中通过分级、乙醇和甲醇/氯仿洗涤和冷冻干燥制备粗细胞壁。通过果胶酶消化和随后在 DEAE-Toyopearl、Superdex-75 和 DEAE-Sephacel 上的柱层析，从粗萝卜细胞壁中溶解和纯化含硼多糖。纯化的多糖分子量为7,000，由糖醛酸52%、腺糖17.4%、半乳糖4.9%和鼠李糖9.8%组成，表明含硼多糖为鼠李糖半乳糖醛酸。 11B NMR 显示，一分子多糖含有一分子硼酸。该多糖中硼的回收率达到细胞壁硼的 80%，表明硼几乎完全以与该多糖结合的形式存在于细胞壁中。我推测硼可能通过与这种多糖结合来发挥其功能。

项目成果

Toru Matoh: "Isolation and Characterization of a Boron-Polysaccharide Complex from Radish Roots" Plant Cell Physiol.34. 639-642 (1993)

Toru Matoh：“萝卜根中硼多糖复合物的分离和表征”植物细胞生理学.34。

Toru Matoh: "Boron Nutrition of Cultured Tobacco BY-2 Cells.I.Requirement for and Intracellular Localization of Boron and Selection of Cells that Tolerate Low Levels of Boron" Plant Cell Physiol.33. 1135-1141 (1992)

Toru Matoh：“培养烟草 BY-2 细胞的硼营养。I.硼的要求和细胞内定位以及耐受低水平硼的细胞的选择”植物细胞生理学.33。

Toru Matoh, Ken-ichi Ishigaki, Kaori Ohno, Jun-ichi Azuma: "Isolation and characterization of a boron-polysaccharide complex from radish roots" Plant & Cell Physiology. 34. 639-642 (1993)

Toru Matoh、Ken-ichi Ishigaki、Kaori Ohno、Jun-ichi Azuma：“萝卜根中硼多糖复合物的分离和表征”植物

Toru Matoh: "Boron Nutrition of Cultured Tobacco BY-2 Cells.I.Requlrement for and Intracellular Localization of Boron and Selection of Cells that Tolerate Low Levels of Boron" Plant Cell Physiol.33. 1135-1141 (1992)

Toru Matoh：“培养烟草 BY-2 细胞的硼营养。I.硼的要求和细胞内定位以及耐受低水平硼的细胞的选择”植物细胞生理学。33。

Toru Matoh, Ken-ichi Ishigaki, Masaharu Mizutani, Keiji Takabe: "Boron Nutrition of cultured tobacco cells BY-2" Plant & Cell Physiology. 33. 1135-1141 (1992)

Toru Matoh、Ken-ichi Ishigaki、Masaharu Mizutani、Keiji Takabe：“培养烟草细胞 BY-2 的硼营养”植物