Biochemistry and physiology of the inorganic nutrients working in the apoplast of higher plants

高等植物质外体中无机营养物质的生物化学和生理学

• 批准号: 13460031
• 负责人: MATOH Toru
• 金额: $ 6.08万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460031/
• 关键词: Bovon; Calcium; Auminium; Apoplast; Cell wall; Edaphic stress; 土壌ストレス; ペクチン; KDO合成酵素; ラムノガラクツロナンII; マイクロアレイ

We have revealed that the essential plant nutrient element boron (B) works to cross-link pectic polysaccharide chains at the rhamnogalacturonan II (RG-II) sites along the chain. In the cross-link, Ca^<2+> ions are also involved to connect the chains, but Al ion interferes with the bonding. Matoh and his colleague purified some enzymes which are responsible for the sugars which particular to RG-II, a KDO synthase, and its DNA was cloned and sequenced. Fujiwara et al revealed that B is actively taken up by higher plants through a B-specific membrane proteins. The protein and its DNA was cloned and sequenced. The B transporter works in the endodermis. Ma studied the binding sites for Al in barley root cell walls. Aluminum ion binds to cell wall pectic polysaccharides, but it works the adverse effect only by which binds to the outer epidermis.

我们已经发现，必需的植物营养元素硼（B）的工程交联果胶多糖链在鼠李糖半乳糖醛酸聚糖II（RG-II）的网站沿着链。在交联过程中，Ca^2+离子也参与了链的连接，但Al离子干扰了键合。Matoh和他的同事纯化了一些负责糖的酶，这些酶是RG-II特有的，一种KDO合酶，其DNA被克隆和测序。Fujiwara等揭示高等植物通过B特异性膜蛋白主动吸收B。对该蛋白及其DNA进行了克隆和测序。B转运蛋白在内皮中起作用。Ma研究了大麦根细胞壁中Al的结合位点。铝离子与细胞壁果胶多糖结合，但仅通过其与外表皮的结合而产生不良作用。

项目成果

Matoh, T.: "Boron function in plant cell walls"Boron in Plant and Animal Nutrition. 143-155 (2002)

Matoh, T.：“硼在植物细胞壁中的功能”植物和动物营养中的硼。

Masaru Kobayashi, et al.: "Purification and cDNA Cloning of UDP-D-Glucuronate Carboxylyase (UDP-D-xylose Synthase) from Pea Seedlings"Plant Cell Physiology. 43. 1259-1265 (2002)

Masaru Kobayashi 等人：“来自豌豆幼苗的 UDP-D-葡萄糖醛酸羧化酶（UDP-D-木糖合酶）的纯化和 cDNA 克隆”植物细胞生理学。

Matoh T and Kobayashi M,: "Boron function in plant cell walls-Research progress since 1997"Boron in Plant and Animal Nutrition (Eds. , Goldbach HE, Rerkasem B, Wimmer MA, Brown PH, Thellier M & Bell RW, Kluwer Academic/Plenum Publishers, New York 2002). 1

Matoh T 和 Kobayashi M，：“硼在植物细胞壁中的功能 - 自 1997 年以来的研究进展”植物和动物营养中的硼（Eds.，Goldbach HE，Rerkasem B，Wimmer MA，Brown PH，Thellier M

Qin Yu, Hlavacka A, Matoh T, Volkmann D, Menzel D, Goldbach HE, Baluska F.: "Short-term boron deprivation inhibits endocytosis of cell wall pectins in meristematic cells of maize and wheat root apices"Plant Physiol. 130. 415-421 (2002)

秦宇，Hlavacka A，Matoh T，Volkmann D，Menzel D，Goldbach HE，Baluska F.：“短期缺硼抑制玉米和小麦根尖分生组织细胞壁果胶的内吞作用”植物生理学。

Qin Yu, Hlavacka: "Short-term boron deprivation inhibits"Plant Physiol.. 130. 415-421 (2002)

秦宇，Hlavacka：“短期缺硼抑制”植物生理学.. 130. 415-421 (2002)