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Studies on salt tolerance mechanisms in halophytes

盐生植物耐盐机制研究

基本信息

批准号：
60560072
负责人：
MATOH Toru
金额：
$ 1.09万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560072/
关键词：
Halophyte Salt tolerance Vacuole Protoplast ホソバノハマアカザ

项目摘要

Seedlings of a coastal halophyte, Atriplex gmelini C.A.Mey were grown in water culture with varying concentrations and species of salts. Plants receiving nutrients supplemented with 50mM NaCl gave the maximum dry weight yield compared with those receiving the higher or the lower concentrations of NaCl. Addition of 50mM KCl, 25mM <Na_2SO_4> , or 25mM <K_2SO_4> to the basal culture solution also stimulated the growth to the same extent. However, higher concentrations of potassium salts, such as than sodium salts did. At a concentration of 250 mM NaCl or KCl addition, the KCl-treated than sodium salts did. At a concentration of 250 mM NaCl or KCl addition, the KCl-treated plants showed a higher sodium plus potassium concentration than the NaCl-treated plants. This phenomenum may be ascribed to the lack of control systems for the maintenance of the internal concentration of potassium, which can operate when sodium salts are supplied.Vacuoles were isolated via protoplasts from the leaves of a halophyte Atriplex gmelini C.A.Mey., grown in culture solution supplemented with 250 mM NaCl. Lysis of the protoplasts was induced by lowering the medium osmolarity and adding a detergent, a synthesized cholate derivative, CHAPS, at a concentration of 0.5 mM and the resulting vacuoles were purified by successive dilution and floatation. Isolated vacuoles contained almost the same concentration of sodium (569 mM) and chloride (260 mM) as recorded in protoplasts (582 mM and 254 mM, respectively), suggesting that the vacuoles are the major sequestration site of NaCl in leaves of halophytes. Betaine concentration in the protoplasts was about 16 mM, while that in vacuoles was only about 0.24 mM, indicating that betaine is accumulated in the cytoplasm as compatible solute.

在具有不同浓度和种类的盐的水培中生长沿海盐生植物滨藜（Atriplex gmelini C.A.Mey）的幼苗。与接受较高或较低浓度的NaCl的植株相比，接受补充有50mM NaCl的营养物的植株得到最大的干重产量。在基础培养液中加入50mM KCl、25mM<Na_2SO_4>或25mM<K_2SO_4>也能刺激生长。然而，更高浓度的钾盐，如比钠盐。在浓度为250 mM的NaCl或KCl的添加下，KCl处理的比钠盐处理的好。在250 mM NaCl或KCl添加浓度下，KCl处理的植物显示出比NaCl处理的植物更高的钠和钾浓度。这一现象可能归因于缺乏维持钾的内部浓度的控制系统，当供应钠盐时，该系统可以起作用。在补充有250 mM NaCl的培养液中生长。通过降低介质渗透压和添加洗涤剂，合成的胆酸盐衍生物，CHAPS，在0.5 mM的浓度诱导原生质体的裂解，并通过连续稀释和浮选纯化所得的液泡。孤立的液泡中含有几乎相同浓度的钠（569 mM）和氯（260 mM）的记录在原生质体（582 mM和254 mM，分别），这表明液泡是主要的隔离网站的NaCl在盐生植物的叶子。甜菜碱在原生质体中的浓度约为16 mM，而在液泡中的浓度仅为约0.24 mM，表明甜菜碱作为相容性溶质积累在细胞质中。