FUNCTION OF BORON IN HIGHER PLANT CELL WALLS

用家兔制备了萝卜根细胞壁硼多糖复合物（BPC）的抗血清。将BPC与甲基化BSA沿着注射，可产生抗血清，并以此建立了尼龙膜免疫印迹法。检测了抗血清的特异性，证明抗血清对BPC具有特异性，而与来源无关。使用抗血清，进行免疫电子显微镜，其无处不在的细胞壁中的定位，但其浓度在细胞壁的角落被揭示。

Antisera against the Boron-polysaccharide complex (BPC) of radish root cell walls were raised using rabbits. The antisera was produced only when the BPC was injected along with methylated-BSA.Using the antisera, immunoblotting for acidic polysaccharides was established employing a nylon membrane. The specificity of the antisera was examined and it was proved that the antisera was specific to the BPC,irrespective of the origin. Using the antisera, immunoelectron microscopy was carried out, and its ubiquitous localization in cell walls, but its concentration in the corner of cell walls was revealed.