Evolutionary relationship of biphenyl-catabolic genes in soil bacteria

土壤细菌联苯分解代谢基因的进化关系

• 批准号: 04660124
• 负责人: FURUKAWA Kensuke
• 金额: $ 1.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660124/
• 关键词: Biphenyl-utilizing bacteria; Toluene-utilizing bacteria; Catabolic genes; Nucleotide sequence; Molecular evolution; Genetic homology; Dioxygenase; Substrate specificity; ビフェニル; 土壌細菌; コスミドライブラリー

Biphenyl(BP) utilizing bacteria are widely distributed in the environment. Thirty two different BP-strains isolated far clasified into four groups according to the homologies of BP-catabolic bph genes and immunological properties of BP-ring-cleavage dioxygenases. The bph operon of Pseudomonas pseudoalcaligenes KF707 is organized as bphA1A2A3A4BCXD.Biphenyl dioxygenase is a multi-component enzyme which composed of large subunit(encoded by bphA1) and small subunit(encoded by bphA2)of terminal deioxygenase, ferredoxin(encoded by bphA3)and ferredoxin reductase(encoded by bphA4). bphB encodes BP-dihydrodiol dehydrogenase, bphC encodes BP-ring meta-cleavage dioxygenase, and bphD encodes hydrolase. The bph operon is very similar to toluene catabolic tod operon of Pseudomonas putida F1 in the gene organization and nucleotide sequences. The gene components responsible for substrate specificity between the bph and tod operons were investigated. The large and small subunits of the terminal dioxygease and the ring meta-cleavage compound hydrolase were critical for their discrete metabolic specificities. From the results obtained in this study it can be postulated that BP-utilizing bacteria, along with other aromatics utilizing bacteira, might be involved in the final degradation of plant lignin.

联苯（BP）利用菌广泛分布于环境中。根据BP分解代谢bph基因的同源性和BP环裂解双加氧酶的免疫学特性，将32株不同的BP菌株分为4类。假产碱假单胞菌（Pseudomonaspseudoalcaligenes）KF 707的bph操纵子结构为bphA 1A 2A 3A 4 BCXD，联苯双加氧酶是由bphA 1编码的末端脱氧酶大亚基和bphA 2编码的末端脱氧酶小亚基、bphA 3编码的铁氧还蛋白和bphA 4编码的铁氧还蛋白还原酶组成的多组分酶。bphB编码BP-二氢二醇脱氢酶，bphC编码BP-环间位裂解双加氧酶，bphD编码水解酶。bph操纵子与恶臭假单胞菌（Pseudomonasputida）F1的甲苯分解代谢tod操纵子在基因结构和核苷酸序列上非常相似。研究了bph和tod操纵子之间负责底物特异性的基因组分。末端双加氧酶和环间位裂解复合物水解酶的大小亚基对它们的离散代谢特异性至关重要。从本研究中获得的结果可以推测，利用BP的细菌，沿着与其他利用芳烃的细菌，可能参与植物木质素的最终降解。

项目成果

K.Furukawa, S.Hayashida and K.Taira: "Biochemical and genetic basis for the degradation of polychlorinated biphenyls in soil bacteria" Pseudomonas : Molecular Biology and Biotechnology. 259-267 (1992)

K.Furukawa、S.Hayashida 和 K.Taira：“土壤细菌中多氯联苯降解的生化和遗传基础”假单胞菌：分子生物学和生物技术。

Furukawa,K.et.al.: "Biochemical and genatic basis for the degradetion of polychlorinated biphenyls in soil bacteria" “Pseudomonas:Molecular Biology and Biotechnology". 259-267 (1992)

Furukawa, K.et.al.：“土壤细菌中多氯联苯降解的生化和遗传基础”“假单胞菌：分子生物学和生物技术”259-267 (1992)。

Furukawa,K.: "Molecular genetics and evolutionary relation-ship of PCB-degrading bacteria" Biodegradation. (印刷中). (1994)

Furukawa, K.：“PCB 降解细菌的分子遗传学和进化关系”《生物降解》（出版中）。

K.Furukawa, J.Hirose, S.Hayashida and K.Nakamura: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol. 176(in press). (1994)

K.Furukawa、J.Hirose、S.Hayashida 和 K.Nakamura：“通过混合芳环双加氧酶有效降解三氯乙烯”J.Bacteriol。

K,Furukawa et al.: "Molecular genetics and evolutionary relationship of PCB-degrading bacteria" Biodegradation. (印刷中). (1994)

K, Furukawa 等人：“PCB 降解细菌的分子遗传学和进化关系”，生物降解（出版中）。