Construction of bioreactors for the degradation of volatile organic chlorinated solvents

降解挥发性有机氯化溶剂的生物反应器的构建

• 批准号: 07556095
• 负责人: FURUKAWA Kensuke
• 金额: $ 1.02万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556095/
• 关键词: tetrachloroethylene; trichloroethylene; Pseudomonas; Anaerobic bacteria; dehalogenation; 脱塩素化; ジオキシゲナーゼ; ハイブリッド酵素; バイオリアクター

Tetrachloroethylene (or perchloroethylene, PCE) and trtichloerothylene (TCE) are known to be serious environmental pollutants in many countries. The present research aimed to develop strains which are able to degrade PCE and TCE efficiently, and construct bioreactors. Since PCE is not able to be degraded by aerobic bacteria, we attempted to isolate anaerobic bacteria and succesfully isolated strain Y51 which showed efficient dechlorination from PCE to cis-1,2-dichloroetnylene (DCE) via TCE.Y51 could grow up to 0.4 at O.D.660 nm in a minimum medium supplemented with 0,2% yeast extract, 50 mM sodium pyruvate and 5 mM fumarate, and dechlorinated PCE completely to DCE at a concentration of as high as 100 ppm. We have also constructed a hybrid dioxygenase from toluene dioxygenase and biphenyl dioxygenase, which showed tremendous degradation capability for TCE and DCE.We demonstrated total degradation of PCE by combining anaerobic Y51 and aerobic Pseudomonas strain D3 expressing the hybrid dioxygenase. The strain D3 could grow well to 1.2*10^9/ml in a glass colmn packed with sand, and degraded TCE (10 ppm) in 5 h. Anaerobic bioreactor using strain Y51, however, remained to be constructed.

四氯乙烯（或全氯乙烯，PCE）和三氯乙烯（TCE）是许多国家已知的严重的环境污染物。本研究旨在培养能够高效降解PCE和TCE的菌株，并构建生物反应器。由于PCE不能被好氧菌降解，我们尝试分离厌氧菌，并成功分离出菌株Y51，该菌株通过TCE有效地将PCE脱氯为顺式-1,2-二氯乙烯（DCE）。在添加0.2%酵母提取物、50 mM丙酮酸钠和5 mM富马酸盐的培养基中，在浓度高达100 ppm时，将PCE完全脱氯为DCE， Y51在O.D.660 nm的最小培养基中可以生长到0.4。我们还构建了甲苯双加氧酶和联苯双加氧酶的杂交双加氧酶，该酶对TCE和DCE具有很强的降解能力。我们通过将厌氧Y51和表达杂交双加氧酶的好氧假单胞菌D3结合，证明了PCE的完全降解。菌株D3在砂填充的玻璃柱中可以很好地生长到1.2*10^9/ml，并在5 h内降解TCE (10 ppm)，但菌株Y51的厌氧生物反应器仍有待构建。

项目成果

Furukawa, K.: "Designing microorganisms for the degradation of environmental pollutants" Bio Japan '96 Symposium Proceedings. 232-241 (1996)

Furukawa, K.：“设计用于降解环境污染物的微生物”Bio Japan 96 研讨会论文集。

Furukawa,K.: "Designing microorganisms for the degradation of environmental pollutants." Bio Japan '96 Symposium Proceedings. 232-241 (1996)

Furukawa,K.：“设计微生物来降解环境污染物。”

古川謙介: "環境微生物の遺伝子伝播と環境適応・進化" Microbes and Environments. 11(1). 19-24 (1996)

Kensuke Furukawa：“环境微生物的基因传播、环境适应和进化”《微生物与环境》11（1）（1996）。

Furukawa, K.: "Gene transfer, enviromental adaptation and evolution" Microbes and Environments. 11(1). 19-24 (1996)

Furukawa, K.：“基因转移、环境适应和进化”微生物与环境。

Furukawa,K.: "Microbial degradation of PCB" OECD Documents Bioremediation. 161-167 (1995)

Furukawa,K.：“PCB 的微生物降解”OECD 文件生物修复。