Studies on biological dehalogenation of chlorinated organic compounds
有机氯化物的生物脱卤研究
基本信息
- 批准号:14360057
- 负责人:
- 金额:$ 9.98万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A strictly anaerobic bacterium Desulfitobacterium sp.Y51 converts tetrachloroethene (PCE) as a final electron acceptor to cis-1,2-dichloroethene (cis-DCE,) via trichloroethene (TCE) very efficiently. This reaction is associated with energy conservation and called as dehalorespiration or halorespiration. We obtained the following results.1.We purified a PCE-dehalogenase (PceA encoded by pceA), and raised polychlonal antibody against purified PceA. Using the antibody we found that PceA is localized at periplasm, associating the cell membrane.2.The production of PceA was highly inducible by addition of PCE or TCE. cis-DCE, on the other hand, inhibited the PceA production at the transcriptional level. The enzyme reaction was also inhibited by cis-DCE.3.We cloned additional genes upstream and downstream regions of pceAB gene cluster. The putatative regulatory pceR gene was found upstream of pceAB. PceR protein was bound with PCE and/or TCE and considered to stimulate the transcription of pceAB gene cluster.4.The commercial cis-DCE contained high amount of chloroform. About 6.6-kb DNA including pceAB genes were highly deleted (more than 80%) when the cells were grown with 1μM chloroform. The cell growth of wild type strain was greatly inhibited, but the growth of deletion mutant was not.5.We obtained a microbial consortium that dechlorinate PCE to ethylene completely in 10 days, in which Dehalococcoides strains, known as a completed dechlorination of various chlroethenes, were present.
严格厌氧细菌脱硫杆菌Y51通过三氯乙烯(TCE)高效地将最终电子受体四氯乙烯(PCE)转化为顺-1,2-二氯乙烯(cis-DCE)。这种反应与能量守恒有关,被称为脱卤素呼吸或卤素呼吸。我们获得了以下结果:1.纯化了PCE-脱卤酶(由pceA编码的PceA),并制备了针对纯化的PceA的多克隆抗体。使用该抗体,我们发现PceA定位于周质,结合细胞膜。2.加入PCE或TCE可高度诱导PceA的产生。另一方面,顺式-DCE在转录水平上抑制PceA的产生。酶反应也被顺式DCE3抑制。3.克隆了pceAB基因簇的上下游基因。在pceAB的上游发现了具有腐烂调节作用的pceR基因。PceR蛋白与PCE和/或TCE结合,被认为能刺激pceAB基因簇的转录。4.商品化的顺式-DCE含有大量的氯仿。当细胞在1μM氯仿中生长时,约6.6kbDNA包括pceAB基因高度缺失(80%以上)。野生型菌株的细胞生长受到很大的抑制,但缺失突变体的生长没有受到抑制。5.我们获得了一个在10天内将PCE完全脱氯为乙烯的微生物联合体,其中存在脱卤球菌属的菌株,即对各种氯乙烯的完全脱氯。
项目成果
期刊论文数量(58)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
有害化学物質を分解するスーパー微生物の開発
开发分解有害化学物质的超级微生物
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Yuji Nakada;Yoshifumi Itoh;古川謙介
- 通讯作者:古川謙介
K.Furukawa et al.: "Engineering microbes capable of efficient degradation of chlorinated organic pollutants"J. Chem. Industry and Engineering. 63. 26-27 (2002)
K.Furukawa等:“能够高效降解氯化有机污染物的工程微生物”J。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase
表达改良樟脑单加氧酶和混合双加氧酶的工程产碱杆菌菌株可完全降解五氯乙烷
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Iwakiri;R. et al.
- 通讯作者:R. et al.
Desulfitobacterium sp.Y51株のクロロエチレン脱ハロゲン化に関する研究
脱硫杆菌Y51菌株的氯乙烯脱卤研究
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:陶山明子;古川謙介
- 通讯作者:古川謙介
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FURUKAWA Kensuke其他文献
FURUKAWA Kensuke的其他文献
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{{ truncateString('FURUKAWA Kensuke', 18)}}的其他基金
Molecular Bases of evolution and degeneration of microbes involved in degradation of environmental pollutants
参与环境污染物降解的微生物进化和退化的分子基础
- 批准号:
22580382 - 财政年份:2010
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
R & D of treatment of soil contaminated with chlorinated organic compounds by combined method of chemical dehalogenation and microbial degradation
化学脱卤与微生物降解复合法处理氯化有机物污染土壤的研发
- 批准号:
11556018 - 财政年份:1999
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Construction of novel metabolic pathway by DNA shuffling of aromatic dioxygenase genes
通过芳香双加氧酶基因的DNA改组构建新的代谢途径
- 批准号:
11460048 - 财政年份:1999
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Construction of bioreactors for the degradation of volatile organic chlorinated solvents
降解挥发性有机氯化溶剂的生物反应器的构建
- 批准号:
07556095 - 财政年份:1995
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
The enhancement of microorganisms for chemical modification
增强微生物化学修饰
- 批准号:
04044204 - 财政年份:1992
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Overseas Scientific Survey.
Evolutionary relationship of biphenyl-catabolic genes in soil bacteria
土壤细菌联苯分解代谢基因的进化关系
- 批准号:
04660124 - 财政年份:1992
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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Challenge to dechlorinate polychlorinated biphenyls under aerobic conditions with an engineered reductive dehalogenase
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18K19881 - 财政年份:2018
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Grant-in-Aid for Challenging Research (Exploratory)
A Reductive Dehalogenase for Aerobic Applications In the Environment
用于环境中有氧应用的还原脱卤酶
- 批准号:
1803771 - 财政年份:2018
- 资助金额:
$ 9.98万 - 项目类别:
Standard Grant
Functional typing of reductive dehalogenase in dehalorespiring bacteria
脱卤呼吸菌还原脱卤酶的功能分型
- 批准号:
17H01900 - 财政年份:2017
- 资助金额:
$ 9.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)