MECHANISM OF CELL DIFFERENTIATION OF ENDOCRINE CELL, WITH SPECIAL REFERENCE TO THE SOMATOMAMMOTROPH IN ANTERIOR PITUITARY GLAND.

内分泌细胞的细胞分化机制，特别是垂体前叶的生长发育细胞。

• 批准号: 04670003
• 负责人: INOUE Kinji
• 金额: $ 0.58万
• 依托单位: FACULTY OF SCIENCE OF SAITAMA UNIVERSITY (1993)Gunma University (1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670003/
• 关键词: PITUITARY GLAND; GROWTH HORMONE CELL; PROLACTIN CELL; SOMATOMAMMOTROPH; INSULIN; INSULIN LIKE GROWTH FACTOR; CELL PROLIFERATION; TRANSDIFFERENCIATION; 下垂体前葉; 成長ホルモン; プロラクチン; 3胞星状細胞

The endocrine gland, such as an anterior gland consist from the mixed population of different hormone secreting glandular cells. These different glandular cells supposedly differentiated from same stem cell however, the mechanism of cell differentiation in endocrine cells still under discusson. We recently established cell line(MtT/S)from rat plaractinoma. The MtT/S cell line produces only growth hormone(GH)in normal growth medium. The MtT/S cells labeled with bromodeoxyuridine(BrdU)also show staining for GH.Furthermore, mitotic MtT/S cells contain GH immunopositive secretory granules in their cytoplasm. These features indicate that MtT/D cells proliferate as GH-secreting cells. We reported previouly that MtT/S cells transdifferentiate into prolactin cells upon stimulation with insulin or insulin-like growth factor. A requirement of estrogen priming for MtT/S cell transdifferentiation has also been reported. This transdifferentiation is considerably accelerated by addition of EGF, resulting in somatomammotrophs contain both prolactin and GH.In order to reveal the relationship between cell proliferation and cell differentiation, a BrdU labeling experiment was performed. the results indicated that prolactin cells which were induced by stimulation with insulin and EGF showed markedly suppressed BrdU incorporation, indicating that prolactin cells that have transdifferentiated from growth hormone cells are non-proliferate or show only very slow proliferation. In general, prolactin cells are known to be the most active proliferative cells in the pituitary gland. This discrepancy between in vitro and in vivo result is verly important, since it may indicate an unknown mechanism for the regulation of prolacrtin cell proliferation in the anterior pituitary gland. On the other hand these cell differentiation may be controlled by neighboring supportive cells through cell cell communication. The folliculo-stellate cells in the anterior pituitary gland is the most important cand

内分泌腺，如前腺，由不同激素分泌腺细胞的混合群体组成。这些不同的腺细胞可能是由同一干细胞分化而来，但内分泌细胞分化的机制仍在讨论中。我们最近建立了大鼠皮肤放线菌瘤细胞系（MtT/S）。MtT/S细胞系在正常生长培养基中仅产生生长激素（GH）。溴脱氧尿苷（BrdU）标记的MtT/S细胞也呈GH染色，有丝分裂期的MtT/S细胞胞浆内含有GH免疫阳性的分泌颗粒。这些特征表明MtT/D细胞作为GH分泌细胞增殖。我们以前报道过MtT/S细胞在胰岛素或胰岛素样生长因子刺激下转分化为催乳素细胞。还报道了MtT/S细胞转分化需要雌激素引发。这种转分化是相当加速的EGF的加入，导致在体乳营养细胞含有催乳素和GH。为了揭示细胞增殖和细胞分化之间的关系，BrdU标记实验进行。结果表明，通过用胰岛素和EGF刺激诱导的催乳素细胞显示出明显抑制的BrdU掺入，表明从生长激素细胞转分化的催乳素细胞是不增殖的或仅显示出非常缓慢的增殖。一般来说，催乳素细胞是脑垂体中增殖最活跃的细胞。体外和体内结果之间的差异非常重要，因为它可能表明垂体前叶中促乳素细胞增殖的调节机制尚不清楚。另一方面，这些细胞分化可以通过细胞通讯由邻近的支持细胞控制。垂体前叶中的滤泡星状细胞是最重要的细胞，

项目成果

Nagata et.al: "Incleased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line,MtT/S." J.Cell.Physiol.15. 426-432 (1992)

Nagata 等人：“在产生生长激素的大鼠垂体细胞系 MtT/S 中诱导催乳素生物合成过程中，肽基精氨酸脱亚胺酶的表达增加。”

Yasuda H., Inoue K., Shibata H., Takeuchi T., Eto Y., Hasegawa Y., Sekine N., Totsuka Y., Mine T., Ogata E.and Kojima I.: "Existence of activin A in A- and D- cells of rat pancreatic islet." Endocrinology. 133. 624-630 (1993)

Yasuda H.、Inoue K.、Shibata H.、Takeuchi T.、Eto Y.、Hasekawa Y.、Sekine N.、Totsuka Y.、Mine T.、Ogata E.和 Kojima I.：“激活素 A 的存在

Matsumoto H., Ishibashi Y., Ohtani T., Hasegawa Y., Koyama C.and K.: "Newly establishied murine pituitary folliculo-stellate-like cell line(TtT/GF)secretes potent pituitary glandular cell survival factors, one of which corresponds to metallopoteinase inhi

Matsumoto H.、Ishibashi Y.、Ohtani T.、Hasekawa Y.、Koyama C. 和 K.：“新建立的鼠垂体滤泡星状细胞系 (TtT/GF) 分泌有效的垂体腺细胞存活因子，是

Matsumoto H.et.al.: "Pituitary folliculo-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pititary adenylate cyclase-activating peptide), showing increased adenosine 3', 5 '-monophosphate and interleukin-6 secretion and cell proli

Matsumoto H.et.al.：“垂体滤泡星状细胞系 (TtT/GF) 对新型促垂体肽（垂体腺苷酸环化酶激活肽）有反应，显示腺苷 3、5-单磷酸和白细胞介素 6 增加

Sakai et al.: "Light and electnon micnoscopic immunocyto chewistny of TSH-like cells occuning in the pans tuberalis of the adult malenat pitaitany" Arch.Histol,Cytal,. 55. 151-157 (1992)

Sakai 等人：“光和电子显微免疫细胞咀嚼对成年雄性马列纳特皮泰坦结节内 TSH 样细胞的免疫细胞咀嚼”Arch.Histol，Cytal，。