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Study on the function of folliculo-stellate cells in the anterior pituitary gland, especially the analysis of their secretory protein.

垂体前叶滤泡星状细胞功能的研究，特别是其分泌蛋白的分析。

基本信息

批准号：
08457257
负责人：
INOUE Kinji
金额：
$ 1.73万
依托单位：
Saitama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The anterior pituitary gland is composed of endocrine cells and supportive cells. The folliculo-stellate cell(FS-cell) is the major cellular element of these supportive cells and expected that ES-cells have very important function in the anterior pituitary gland. However, the precise function of FS cells in the anterior pituitary gland has not been established. The purpose of this research project is a study of the function of folliculo stellate cells. We first noticed the appearance of large number of PAS positive colloid in the senescent porcine pituitary gland Our morphological study revealed that these PAS positive colloids were completely surrounded by the FS cells. Therefore, the characterization of the colloidal substance may be important for the analysis of FS cell function. Fortunately we have succeeded in purifying and analyzing the collois using the porcine pituitary gland and revealed the colloids contained large amount of clusterin. The clusterin is known as the highly glycosilated protein and suspected that it may be related to the neural cell apoptosis in the brain. Our immunocytochemical study showed clusterin is not produced by FS cells but some endocrine cells. These results suggest the cells produced chusterin were undergone to the apoptosis and phagocytosed by the FS cells and the clusterin accumulated in the colloid as the residual body. The colloid appearance in the pituitary gland is well known phenomenon however this is the first study which revealed the chemical component of those colloids, For the analysis the biological significance of the clusterin appearance in the pituitary gland is need further study.

垂体前叶由内分泌细胞和支持细胞组成。卵泡星状细胞（FS细胞）是这些支持细胞的主要细胞成分，预期ES细胞在垂体前叶中具有非常重要的功能。然而，FS细胞在垂体前叶的确切功能尚未确定。本研究的目的是研究毛囊星状细胞的功能。我们首次在衰老的猪垂体中发现大量PAS阳性胶体的出现，形态学研究表明这些PAS阳性胶体被FS细胞完全包围。因此，胶体物质的特性可能是重要的FS细胞功能的分析。幸运的是，我们已经成功地利用猪垂体纯化和分析了胶体，发现胶体中含有大量的丛生蛋白。clusterin是一种高度糖基化的蛋白质，推测其可能与脑内神经细胞凋亡有关。我们的免疫细胞化学研究表明聚集素不是由FS细胞产生的，而是由某些内分泌细胞产生的。这些结果表明，产生chusterin的细胞发生凋亡，被FS细胞吞噬，聚集在胶体中作为残留体。脑垂体中胶质的出现是众所周知的现象，但这是首次揭示胶质的化学成分，其生物学意义有待进一步研究。