Study of The Mechanism of Prolactin Cell Differentiation in The Anterior Pituitary

垂体前叶催乳素细胞分化机制的研究

• 批准号: 02807001
• 负责人: INOUE Kinji
• 金额: $ 1.02万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807001/
• 关键词: Pituitary; Growth hormone; Prolactin; Transdifferentiation; Differentiation; Insulin; Insulin-Like-Growth Factor; Somatomammotroph; 下垂体前葉; 成長ホルモン細胞; プロラクチン細胞; 転化; EGF; ソマトマンモトロフ; 培養細胞; 内分泌細胞

Newly established MtT/S-secrete only the rowth hormone(GH). Electron microscopic examination of the MTT/S cells showed a normal somatotroph-like appearance i. e. ., the presence of many growth hormone containing secretory granules, well developed endoplasmic reticulum and Golgi apparatus in their cytoplasm. GRH stimulate GH secretion of MtT/S cell line at the plateau-phase of cell growth. Interestingly, the MTT/S cells in the mitotic phase also contained secretory granules. Furthermore, all of the bromo-deoxyuridine(BrdU)incorporated cells which known an indicator of proliferative cells are growth hormone cells. These characteristics of MTT/S cells are showing this cells proliferate as the GH-cells. No' prolactin(PRL)-cells were observed among MTT/S cells cultured in regular medium, and no PRL in medium and cells was detected with radioimmunoassy and Western blotting analyses, whereas insulin or insulin-like growth factor-1(IGF-1)exerted a marked decrease-in. GH-production and GH-cell … More population. In the medium PRL was detected after a 2-day lag period and a numerous PRecells appeared 5 days after stimulation with insulin or IGF-1. Double immunocytochemistry clearly indicated that no cell contained both PRL and GH. These results show that PRecell transdifferentiated from GH-cells by stimulation with insulin or IGF-1 and this suggests that the MTT/S cell line is an excellent model system showing GHcell/PRecell linea, e. Recent information of the molecular biology indicated that the mammotroph in pituitary cell may-differentiate from somatotroph. However, intra- or extra-cellular mechanism regulating cell differentiation of mammotroph from somatotroph remain unclear. Our newly established MTT/S cell line seems good model for reveal the mechanism pituitary cell differentiation.On the other hand, by the addition of EGF with insulin, the transdifferentiation of GH cells to prolactin is very accelerated and somatomammotroph which have both GH and prolactin were appeared. The existence of somatomammotroph in the pituitary gland is well known however, the meaning of the somatomammotroph in the pituitary gland are unclear. Our result of the appearance of somatomammotroph by the stimulation of insulin and EGF may show somatomammotroph appeared on the process of transdifferentiation of GH-cells to prolactin cells. Less

新建立的MtT/S-仅分泌生长激素（GH）。MTT/S细胞的电镜检查显示正常的生长激素样外观。e. .,细胞质中有大量含生长激素的分泌颗粒，内质网和高尔基体发达。GRH刺激MtT/S细胞生长平台期GH分泌。MTT/S细胞在有丝分裂期也含有分泌颗粒。此外，所有的溴脱氧尿苷（BrdU）掺入的细胞，已知的增殖细胞的指标是生长激素细胞。MTT/S细胞的这些特征表明该细胞具有GH-细胞的增殖特性。MTT/S细胞在常规培养液中未观察到催乳素（PRL）阳性细胞，放射免疫分析和Western blotting分析均未检测到细胞和培养液中PRL的表达，而胰岛素或胰岛素样生长因子-1（IGF-1）的表达则明显减少。生长激素产生和生长激素细胞 ...更多信息 人口在培养基中，PRL在2天的滞后期后被检测到，并且在用胰岛素或IGF-1刺激后5天出现大量的PRecells。双重免疫细胞化学清楚地表明，没有细胞含有PRL和GH。这些结果表明，通过用胰岛素或IGF-1刺激，PRecell从GH-细胞转分化，这表明MTT/S细胞系是显示GH细胞/PRecell系a，e.近年来的分子生物学研究表明，垂体细胞中的促乳腺激素细胞可能是由促生长激素细胞分化而来。然而，细胞内或细胞外调节细胞分化的促乳细胞从生长激素细胞仍不清楚。我们建立的MTT/S细胞系是揭示垂体细胞分化机制的良好模型，同时加入EGF和胰岛素，可显著促进GH细胞向催乳素的转分化，并出现既含GH又含催乳素的生长催乳细胞。垂体中存在促生长乳细胞是众所周知的，然而，促生长乳细胞在垂体中的意义尚不清楚。胰岛素和EGF刺激下生长激素细胞出现的结果提示生长激素细胞转分化为催乳素细胞的过程中出现了生长激素细胞。少

项目成果

K.Inoue et al.: "Establishment of a series of pituitary clonal cell lines differing in morphology.hurmone secretion,and response to estrogen." Endocrinology. 126. 2313-2320 (1990)

K.Inoue 等人：“建立了一系列形态、激素分泌和对雌激素反应不同的垂体克隆细胞系。”

K. Inoue, M-A. Hattori, T. Sakai, S. Inukai, N. Fujimoto, and A. Ito: "Establishment of a series of pituitary clonal cell lines differing in morphology, m hormone secretion, and response to estrogen." Endocrinology. 126. 2313-2320 (1990)

K.井上，M-A。

K.Inoue and T.Sakai: "Conversion of grouth hurmone-secreting cells into prolaction-secreting cells and its propnotion by insulin and insulin-likegrouth factur-1 in viro" Exp.Cell.Res.195. 53-58 (1991)

K.Inoue 和 T.Sakai：“生长激素分泌细胞向催乳分泌细胞的转化及其在体外由胰岛素和类胰岛素生长因子 1 的支持”Exp.Cell.Res.195。

K.INOUE et al.: "Establishment of a Series of pituitary clonal cell lines differing in morphology,hormone secretion,and response to estrogen" Endocrinology. 126. 2313-2320 (1990)

K.INOUE 等人：“建立一系列形态、激素分泌和对雌激素反应不同的垂体克隆细胞系”内分泌学。

S.Nagata et al.: "Estrogen regulates peptidylargine deiminase level in a rat pituitary cell line in culture" J.Cell.Physiol.145. 333-339 (1990)

S.Nagata 等人：“雌激素调节培养的大鼠垂体细胞系中的肽基精氨酸脱亚氨酶水平”J.Cell.Physiol.145。