Research of experimental pathology on the model for ulcerative colitis - large bowel cancer

1)溃疡性结肠炎-结肠癌动物模型的建立：给大鼠灌胃1%L-羟基蒽醌(HA*)460天。在29只有效的动物中，有25只发生了大肠肿瘤(腺瘤或腺癌)。所有这些有效的大鼠都出现了一些在溃疡性结肠炎患者中常见的病理变化。它们是隐窝脓肿、黑变病、糜烂或再生上皮。这些结果表明，HA*可同时引起与溃疡性结肠炎和肿瘤相似的病理变化。2)给予HA*的大鼠增殖上皮细胞的细胞动力学分析：在开始饮食暴露4、8或12个月后，连续观察暴露于HA*(1%)的大鼠的大肠。S…的厚度、构成隐窝的细胞数量、隐窝细胞内BrdU的标记指数、ODC活性和变化的外观HA组大鼠大肠粘膜糜烂、隐窝脓肿发生率均高于对照组。各生物标志物随HA暴露时间的延长而增大。3)1，8-二氢黄原酮(丹参酮)对小鼠大肠肿瘤的促癌作用：观察丹参酮对二甲基肼(DMH)诱导的小鼠大肠癌的抑制作用。服用DMH和丹参酮的小鼠的结肠癌(腺瘤或腺癌)发生率明显高于单独服用DMH的小鼠。给予丹参酮的小鼠大肠组织中ODC活性明显高于对照组。用RT-PCR方法检测细胞因子、肿瘤坏死因子-α和IL-1α在MAM醋酸酯和HA致癌过程中的表达。细胞因子在结肠癌中的表达强于非肿瘤性粘膜。给予两种药物或其中一种药物的结肠粘膜中细胞因子的含量也高于对照组。HA*与MAM醋酸酯的协同作用可能与HA*所产生的炎症状态中表达的细胞因子的生物学效应有关。较少

1) Development of the model for ulcerative colitis - large bowel cancer : Dietary administration of l-hydroxyanthraquinone (HA*) (1%) to rats for approximately 460 days was done. Twenty five rats out of 29 effective animals developed large bowel neoplasms (adenoma or adenocarcinoma). All of these effective rats also developed some pathological changes which are often seen in ulcerative colitis patients. They were crypt abscess, melanosis, erosion or regenerative epithels. These results indicate that HA* induces simultaneously pathological changes resembling those appeared in the case of ulcerative colitis and neoplasms.2) Cell kinetic analysis of proliferative epithelial cells in rats given HA* : Sequential observation of the large bowels of rats exposed to HA* (1%) by sacrifice of animals at 4, 8 or 12 months after the start of tye dietary exposure. Thickness, number of cells constituting crypt, labelling indices of BrdU in the crypt cells, ODC activity and appearance of the changes s … More uch as erosion, crypt abscess of the large bowels of rats with HA* were respectively greater than those of control animals. Each of the biomarkers became greater responding to the exposure-time of HA*.3) Tumor-promotive effect of 1, 8-dihydroxianthraquinone (danthron) in the large of mice : Modifying effect of danthron on dimethylhydrazine (DMH)-induced large bowel carcinogenesis in mice was examined. Incidence of colon neoplasms (adenoma or adenocarcinoma) in mice given DMH together with danthron was significantly higher than that of mice exposed to DMH alone. ODC activity of the large bowel of mice given danthron was much greater than that of controls.Expression of cytokines, TNF-a and IL-la in the large bowel carcinogenesis by MAM acetate and HA* : Expression of the cytokines was examined by RT-PCR method. Expression of the cytokines in the colon neoplasms was stronger than in non-neoplastic mucosa. That of cytokines in the colon mucosa given both agents or either of the agents was aloso greater than in controls. The synergistic effects of HA* with MAM acetate might be related to the biological effects of the cytokines expressed in the inflammatory conditioned generated by HA*. Less