Identification of anti-HTLV-I neutralizing antibodies and epitopes

抗HTLV-I中和抗体和表位的鉴定

• 批准号: 04670277
• 负责人: SONODA Shunro
• 金额: $ 1.34万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670277/
• 关键词: HTLV-I; Mother-to-child transmission; Transplacental antibody; Neutralizing antibody; Anti-env-antibody; 中和抗体; エンベロープ; ウエスタンブロッド; 母子感染防御

We have reported a role of anti-HTLV-I maternal antibodies to protect children from HTLV-I infection after birth (Int.J.Cancer 49, 673-677, 1991).The present study aimed to investigate the antibodies to neutralize HTLV-I and the correlation with anti-envelope antibodies. The mnaternal antibodies disappeared by 3-9 moths after birth. It was confirmed that 30% of chilren born to HTLV-I carrier mothers were infected with HTLV-I when they received breast milk over 6 months after birth (Asia-Occeanian J.Obstet.Gynecol.18, 371-377, 1992).All the children who were not infected with HTLV-I (5/5 cases) shoed poitive neutralizing antibody as well as anti-envelope antibody, while those infected with HTLV-I showed incosistent results of neutralizing a antibodies (4/5 cases) and anti-envelope antibodies (1/5 cases).These results suggested that the presence of anti-envelope antibody might be important for protecting HTLV-I infection. The neutralizing antibodies appeared to react with a variety of epitopes which might be composed of envelopes and non-envelope consituents, the former being the major target of protective antibody and the latter serving an uniquetarget to inhibit HTLV-I replication. The role of anti-envelope antibody is now under confirmation by using serum samples obtained from various ethnic groups other than Japanese (AIDS Res Human Retroviruses 10, 97-101, 1994).

我们报道了抗HTLV-I母体抗体在出生后保护儿童免受HTLV-I感染的作用（Int.J.Cancer 49，673-677，1991）。目前的研究旨在研究中和HTLV-I的抗体，并与抗Envelope抗体抗体相关。出生后，mnernal抗体以3-9个飞蛾消失。有人证实，在出生后6个月内收到母乳时，HTLV-1承运人母亲出生的奇尔伦（Chilren）中有30％感染了HTLV-I（亚太地区J.Obstet.gynecol.18，371-377，371-377，1992）。抗体虽然感染了HTLV-I的抗体表现出中和抗体（4/5例）和抗Envelope抗体（1/5病例）的不辅助结果。这些结果表明，抗Envelope抗体的存在对于保护HTLV-I感染可能很重要。中和抗体似乎与可能由信封和非固定助因的多种表位反应，前者是保护性抗体的主要靶标，而后者则是抑制HTLV-i复制的独立性。现在，使用从日本以外的其他种族群中获得的血清样品（AIDS RES人类逆转录病毒10，97-101，1994），抗启动抗体的作用正在确认。

项目成果

S.Sonoda,B.Arce-Gomez M.L.Satz et al.: "Oxford University Press (eds.K.Tsuji,M.Aizawa,T.Sasazuki)" HLA 1991. 720 (1992)

S.Sonoda、B.Arce-Gomez M.L.Satz 等人：“牛津大学出版社（eds.K.Tsuji、M.Aizawa、T.Sasazuki）” HLA 1991. 720 (1992)

S.Sonoda,B.Arce-Gomez,M.L.Satz et al.: "HLA1991" Oxford University Press.(eds.K.Tsuji,M.Aizawa,T.Sasazuki), 720(12) (1992)

S.Sonoda、B.Arce-Gomez、M.L.Satz 等人：“HLA1991”牛津大学出版社。（eds.K.Tsuji、M.Aizawa、T.Sasazuki），720(12) (1992)

M.Setoyama,Y.Katahira H.Tozawa,S.Sonoda et al.: "Expression of human T-cell Iymphotropic virus type-I gene products in the short-term cultured skin tissues of an adult T-cell leukemia/Iymphoma patient with cutaneous manifestations." J.Dermatol. 19. 133-13

M.Setoyama、Y.Katahira H.Tozawa、S.Sonoda 等人：“人类 T 细胞嗜淋巴细胞病毒 I 型基因产物在成人 T 细胞白血病/淋巴瘤患者的短期培养皮肤组织中的表达

S.Sonoda, B.Arce-gomez, M.L.Satz, et al.: Oxford UniversityPress.(eds.K.Tsuji, M.Aizawa, T.Sasazuki). HLA 1991, 720 (1992)

S.Sonoda、B.Arce-gomez、M.L.Satz 等人：牛津大学出版社（eds.K.Tsuji、M.Aizawa、T.Sasazuki）。

S.Sonoda: Japanese Cancer Association.(eds Takatsuki, Hinuma, Yoshida).Advances in Adult T-cell Leukemia and HTLV-I Research., 261 (1992)

S.Sonoda：日本癌症协会。（编辑 Takatsuki、Hinuma、Yoshida）。成人 T 细胞白血病和 HTLV-I 研究进展。，261 (1992)