Challenge to determine the novel host defense system in male reproductive organs and sperm in chickens

确定鸡雄性生殖器官和精子中新型宿主防御系统的挑战

• 批准号: 22658083
• 负责人: YOSHIMURA Yukinori
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658083/
• 关键词: 畜産学; 繁殖; 宿主防衛; ニワトリ; 精子; 宿主防錆; 抗菌ペプチド; サイトカイン; 授精; 雄生殖器; Toll様受容体

The aim of this study was to determine the innate immune system in the male reproductive organs and sperm of chickens. It was examined whether Toll-like receptors(TLRs) and avianβ-defensins(avBDs) were expressed in male reproductive organs, whether stimulation of TLR induced proinflammatory cytokines, followed by influx of T cells, and whether sperm expressed avBDs in response to microbial components. The results showed that male reproductive organs expressed various TLRs and avBDs, and stimulation of TLR4 by lipopolysaccharide induced proinflammatory cytokines. The synthesized proinflammatory cytokines may enhance cellular immunity mediated by T cells and symthesis of avBDs. Sperm also expressed avBDs that may play roles in host immunity for themselves. The immune functions mediated by TLRs and avBDs may form the host defense system in male reproductive organs and sperm.

本研究的目的是确定鸡雄性生殖器官和精子中的先天免疫系统。研究Toll样受体(Toll样受体，TLRs)和禽类β防御素(AvBds)是否在雄性生殖器官中表达，TLRs刺激是否诱导促炎细胞因子，随后T细胞内流，以及精子是否表达avBD对微生物成分的响应。结果表明，雄性生殖器官表达多种TLRs和avBD，内毒素刺激TLR4产生促炎细胞因子。合成的促炎细胞因子可增强T细胞介导的细胞免疫，促进avBDS的合成。精子也表达avBD，这些avBD可能对自身的宿主免疫起作用。TLRs和avBDS介导的免疫功能可能在男性生殖器官和精子中形成宿主防御系统。

项目成果

新動物生殖学

新动物生殖科学

• 发表时间: 2011
• 作者: 眞鍋昇;他;眞鍋昇
• 通讯作者: 眞鍋昇

Studies on the Innate Immune System in the Male Reproductive Organs in Chickens.

鸡雄性生殖器官先天免疫系统的研究。

• 发表时间: 2010
• 作者: Watanabe Y;Isobe N;Yoshimura;Y
• 通讯作者: Y

ニワトリ雄生殖器の宿主防衛機能

鸡雄性生殖器官的宿主防御功能

• 发表时间: 2011
• 作者: Zhang M;Isobe N;Yoshimura Y;張明・渡辺陽子・磯部直樹・吉村幸則
• 通讯作者: 張明・渡辺陽子・磯部直樹・吉村幸則

Studies on the innate immune system in the male reproductive organs in chickens

鸡雄性生殖器官先天免疫系统的研究

• 发表时间: 2010
• 作者: Watanabe Y;Isobe N;Yoshimura;Y
• 通讯作者: Y

Expression of Toll-like receptors and effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokine in the testis and epididymis of rooster

公鸡睾丸和附睾中Toll样受体的表达及脂多糖对促炎细胞因子和趋化因子表达的影响

• 发表时间: 2012
• 期刊: Poultry Science
• 影响因子: 4.4
• 作者: Zhang M;Nii T;Isobe N;Yoshimura Y
• 通讯作者: Yoshimura Y