Challenge to determine the novel host defense system in male reproductive organs and sperm in chickens

确定鸡雄性生殖器官和精子中新型宿主防御系统的挑战

• 批准号: 22658083
• 负责人: YOSHIMURA Yukinori
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658083/
• 关键词: 畜産学; 繁殖; 宿主防衛; ニワトリ; 精子; 宿主防錆; 抗菌ペプチド; サイトカイン; 授精; 雄生殖器; Toll様受容体

The aim of this study was to determine the innate immune system in the male reproductive organs and sperm of chickens. It was examined whether Toll-like receptors(TLRs) and avianβ-defensins(avBDs) were expressed in male reproductive organs, whether stimulation of TLR induced proinflammatory cytokines, followed by influx of T cells, and whether sperm expressed avBDs in response to microbial components. The results showed that male reproductive organs expressed various TLRs and avBDs, and stimulation of TLR4 by lipopolysaccharide induced proinflammatory cytokines. The synthesized proinflammatory cytokines may enhance cellular immunity mediated by T cells and symthesis of avBDs. Sperm also expressed avBDs that may play roles in host immunity for themselves. The immune functions mediated by TLRs and avBDs may form the host defense system in male reproductive organs and sperm.

这项研究的目的是确定男性生殖器官和鸡精子中的先天免疫系统。检查了在男性生殖器官中是否表达了Toll样受体（TLRs）和AVIANβ-防御素（AVBD）是否表达，是否刺激TLR诱导的促炎细胞因子，然后是T细胞的影响，以及对微生物成分的响应而表达的精子表达AVBD。结果表明，雄性器官表达了各种TLR和AVBD，以及脂多糖诱导的促炎细胞因子刺激TLR4。合成的促炎细胞因子可以增强由T细胞介导的细胞免疫学和AVBD的符号。精子还表达了可能在宿主免疫学中发挥作用的AVBD。由TLR和AVBD介导的免疫功能可能在男性生殖器官和精子中形成宿主防御系统。

项目成果

新動物生殖学

新动物生殖科学

• 发表时间: 2011
• 作者: 眞鍋昇;他;眞鍋昇
• 通讯作者: 眞鍋昇

Studies on the Innate Immune System in the Male Reproductive Organs in Chickens.

鸡雄性生殖器官先天免疫系统的研究。

• 发表时间: 2010
• 作者: Watanabe Y;Isobe N;Yoshimura;Y
• 通讯作者: Y

Studies on the innate immune system in the male reproductive organs in chickens

鸡雄性生殖器官先天免疫系统的研究

• 发表时间: 2010
• 作者: Watanabe Y;Isobe N;Yoshimura;Y
• 通讯作者: Y

ニワトリ雄生殖器の宿主防衛機能

鸡雄性生殖器官的宿主防御功能

• 发表时间: 2011
• 作者: Zhang M;Isobe N;Yoshimura Y;張明・渡辺陽子・磯部直樹・吉村幸則
• 通讯作者: 張明・渡辺陽子・磯部直樹・吉村幸則

Detection of Avian Beta-defensins mRNA and Proteins in Male Reproductive Organs in Chicken

鸡雄性生殖器官中禽β防御素mRNA和蛋白的检测

• DOI: 10.2141/jpsa.011042
• 发表时间: 2011
• 期刊: The Journal of Poultry Science
• 作者: Watanabe Y;Isobe N;Yoshimura Y
• 通讯作者: Yoshimura Y