A study on expression and function of surface molecules of immunocompetent cells in rheumatoid arthritis.

类风湿性关节炎免疫活性细胞表面分子表达及功能研究

• 批准号: 04670378
• 负责人: INOUE Tetsufumi
• 金额: $ 1.28万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670378/
• 关键词: LFA-1; ICAM-1; CD44; IL-10; IL-2; IL-2 receptor; 慢性関節リウマチ; ヒアルロン酸; IL-6

Expresssion of LFA-1, ICAM-1, and CD44 molecules on peripheral blood T lymphocytes were studied. No differences were found between RA patients and healthy individuals. Effects of several therapeutic drugs for RA on the expression of thode molecules were also analized. In the series of experiments, dexamethasone clearly decreased the expression of ICAM-1, suggesting the possibility that anti-rheumatic drugs showed their clinical effects through the modification on such molecules.Anti CD44 antibodies enhanced the release of IL-2 and IL-6 from activated T lymphocytes. Aggregation of cells by anti-CD44 antibodies was also observed in the culture, indicating that CD44 molecules showed the role of signal transmission through bringing T lymphocytes in contact with each other.IL-10 enhanced the proliferation and differentiation of activated B lymphocytes in the presence of IL-2, by means of the accelerated expression of IL-2 receptor molecules.

研究LFA-1、ICAM-1和CD44分子在外周血T淋巴细胞上的表达。类风湿关节炎患者和健康人之间没有发现差异。分析了几种RA治疗药物对阳性分子表达的影响。在一系列实验中，地塞米松明显降低了ICAM-1的表达，提示抗风湿药物可能是通过对ICAM-1分子的修饰来发挥其临床作用的。抗CD44抗体增强活化T淋巴细胞IL-2和IL-6的释放。在培养中也观察到抗CD44抗体对细胞的聚集，表明CD44分子通过使T淋巴细胞相互接触发挥了信号传递的作用。IL-10通过加速IL-2受体分子的表达，增强了活化的B淋巴细胞在IL-2存在下的增殖和分化。

项目成果

S.Hirohta, S.Shinohara, T.Inoue, et.al.: "Regulation of B cell function by Lobenzarit, a novel disease-modifying antirheumatic drug." Arthritis Rheum.35. 168-175 (1992)

S.Hirohta、S.Shinohara、T.Inoue 等人：“Lobenzarit（一种新型缓解疾病抗风湿药物）对 B 细胞功能的调节”。

K.Itoh, Y.Nishioka, T.Inoue, et.al.: "HIV induced systemic lupus erythematosus." Lupus. 3. 205-206 (1994)

K.Itoh、Y.Nishioka、T.Inoue 等人：“HIV 诱发的系统性红斑狼疮。”

HIROHATA,S.,SHINOHARA,S.,INOUE,T.,MIYAMOTO,T.,LIPSKY,P.: "REGULATION ON OF B CELL FUNCTION BY LOBENZARIT,A NOVEL DISEASE MODIFYING ANTIRHEMATIC DRUG." ARTHRITIS RHEUM.35. 168-175 (1992)

HIROHATA,S.、SHINOHARA,S.、INOUE,T.、MIYAMOTO,T.、LIPSKY,P.：“洛本扎利（一种新型抗病药物）对 B 细胞功能的调节。”

SHINOHARA,S.,HIROHATA,S.,INOUE,T.,ITO,K.: "PHENOTYPIC ANALYSIS OF PERIPHERAL BLOOD MONOCYTES ISOLATED FROM PATIENTS WITH RHEUMATOID ARTHRITIS." J.RHEUMATOL.19. 211-215 (1992)

Shinohara,S.、Hirohata,S.、Inoue,T.、ITO,K.：“从类风湿性关节炎患者中分离的外周血单核细胞的表型分析。”

S.Hirohata,T.Inoue他: "Regulation of B cell function by Lobenzarit,a novel antirheumatic drug." Arthritis Rheum.35. 168-175 (1992)

S. Hirohata、T. Inoue 等人：“新型抗风湿药物 Lobenzarit 对 B 细胞功能的调节。”168-175 (1992)。