Investigation of the bile formation in the liver : basic analysis for transplantation

肝微循环、肝实质细胞功能和胆小管功能对胆汁形成有重要影响。再灌注损伤是肝移植中常见的损伤，其发生机制尚不清楚。累积的数据强烈怀疑，受损的肝脏微循环在这一过程中起作用。本课题研究了Ito细胞和Kupffer细胞在肝脏微循环中的作用，发现Ito细胞具有收缩活性，并调节肝血流量。这种收缩活动受肌动蛋白-肌球蛋白系统控制。枯否细胞具有吞噬活性，这也是由细胞骨架系统控制的，并阻断肝血流。因此，维持肝实质细胞的功能对肝移植的成功至关重要。本研究还探讨了肝实质细胞内钙离子的作用，发现细胞内钙离子在胆汁形成和肝再生中起重要作用。

The bile formation is well inferenced by hepatic microcirculation, parenchymal cell function and bile canalicular function. Re-perfusion injury which is commonly occurred in liver transplantation and the mechanism or re-perfusion injury is still unknown. Accumulated data strongly suspected that damaged hepatic microcirculation plays a role on this process. In this project, we investigated the role of Ito cells and Kupffer cells on hepatic microcirculation and revealed that Ito cells had contractile activity and modulated hepatic blood flow. And this contratile activity was controlled by actin-myosin system. Kupffer cells had phagocytic activity which was also controlled by the cytoskeletal system and blocked hepatic blood flow. Therefore, the maintenance of the function of these cells was important for the success of liver transplantation.In this study, the role of calcium in parenchymal hepatocytes was also investigated and revealed that intracellular calcium playd an important role on bile formation and liver regeneration.