Investigation of the bile formation in the liver : basic analysis for transplantation

肝脏胆汁形成的研究：移植的基础分析

• 批准号: 04670443
• 负责人: WATANABE Sumio
• 金额: $ 1.34万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670443/
• 关键词: liver transplantation; bile; microcirculation; Ito cell; Kupffer cell; calcium; cytoskeleton; eudothelin; 肝移植; 胆汁; 微小循環; 伊東細胞; クッパー細胞; カルシウム; 細胞骨格; エンドセリン; 毛細胆管; 胆汁分泌

The bile formation is well inferenced by hepatic microcirculation, parenchymal cell function and bile canalicular function. Re-perfusion injury which is commonly occurred in liver transplantation and the mechanism or re-perfusion injury is still unknown. Accumulated data strongly suspected that damaged hepatic microcirculation plays a role on this process. In this project, we investigated the role of Ito cells and Kupffer cells on hepatic microcirculation and revealed that Ito cells had contractile activity and modulated hepatic blood flow. And this contratile activity was controlled by actin-myosin system. Kupffer cells had phagocytic activity which was also controlled by the cytoskeletal system and blocked hepatic blood flow. Therefore, the maintenance of the function of these cells was important for the success of liver transplantation.In this study, the role of calcium in parenchymal hepatocytes was also investigated and revealed that intracellular calcium playd an important role on bile formation and liver regeneration.

肝微循环、肝实质细胞功能和胆小管功能对胆汁形成有重要影响。再灌注损伤是肝移植中常见的损伤，其发生机制尚不清楚。累积的数据强烈怀疑，受损的肝脏微循环在这一过程中起作用。本课题研究了Ito细胞和Kupffer细胞在肝脏微循环中的作用，发现Ito细胞具有收缩活性，并调节肝血流量。这种收缩活动受肌动蛋白-肌球蛋白系统控制。枯否细胞具有吞噬活性，这也是由细胞骨架系统控制的，并阻断肝血流。因此，维持肝实质细胞的功能对肝移植的成功至关重要。本研究还探讨了肝实质细胞内钙离子的作用，发现细胞内钙离子在胆汁形成和肝再生中起重要作用。

项目成果

Segawa O.et al.: "Actin and myosin deposition around bile canaliculi : A predictor of clinical outcome in biliary atresia" J.Pediat Surg.28. 851-856 (1993)

Sekawa O.等人：“胆小管周围的肌动蛋白和肌球蛋白沉积：胆道闭锁临床结果的预测因子”J.Pediat Surg.28。

北村庸雄他: "21世紀を目指し羽ばたく消化器病学" 螢光標識胆汁酸の胆細胞内輸送機構, 516 (1993)

Tsuneo Kitamura 等：《迈向 21 世纪的胃肠病学》胆汁细胞中荧光标记胆汁酸的转运机制，516 (1993)

Kitamura T.et al.: "Canalicular transport mechanism of fluorescent bile acid in hepatocyte doublets" Gastroenterol Jpn.27. 428 (1992)

Kitamura T.等人：“肝细胞双联体中荧光胆汁酸的小管转运机制”Gastroenterol Jpn.27。

Kenichi Ikejima et al: "Marked reteusion of indocyanine green and Sulfobron phthalein with chronic persistent hepafifis" J.Gastroent Hepatol. 8. 489-494 (1993)

Kenichi Ikejima 等人：“吲哚菁绿和 Sulfobron 酞与慢性持续性肝纤维化的显着重叠”J.Gastroent Hepatol。

Takashi Ueno et al: "Partial characterization of a rabbit liver Ca^<2+>-calmodulin dependent kinase with myosin light chain phosphorylation activity." Biochem Molecul Int. 29. 1145-1152 (1993)

Takashi Ueno 等人：“具有肌球蛋白轻链磷酸化活性的兔肝 Ca^2-钙调蛋白依赖性激酶的部分表征。”