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Study on the subendocardial ischemia using a high resolution needle-probe video-microscope with a CCD camera

使用带有 CCD 摄像头的高分辨率针探针视频显微镜研究心内膜下缺血

基本信息

批准号：
04670569
负责人：
GOTO Masami
金额：
$ 1.34万
依托单位：
Kawasaki College Of Allied Health Professions
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

To understand the basis of the subendocardial vulnerability to ischemia, we evaluated the behavior of the microvessels and their blood flows in the subendocardium using a portable needle-probe video-microscope with a charge-coupled device (CCD) camera. The tip of the needle-probe was introduced into the left ventticular cavity from the left atrium of the beating canine hearts and observed the subendocardial arterioles and venules. To accentuate the small vascular images, we employed : 1) a green (complimentary color of red) filter, 2) an intracoronary injection of FITC-dextran, and 3) an intracoronary or intravenous injection of indocyanine green. Using these techniques, the arterial and venous microvessels could be identified easily, and the diameter changes could be evaluated.We evaluated the effects of ninoglycerin on the coronary microvessels. The study showed that nitroglycerin dilated both subepicardial and subendocardial arterioles. The vasodilatory effects of nitroglycerin on the subendocardial arterioles were different depending on the sizes of the vessels. Nitroglycerin dilated larger arterioles with diameters greater than 100 mum compared to those with diameters smaller than 100 mum. Nitroglycerin increased the pulsation amplitude of the subendocardial arterioles. It was also found that nitroglycerin tended to dilate the coronary venules. Further investigation will be needed to understand the effects of nitroglycerin on the venous myocardial vessels.Using a high-contrast blood flow marker, India ink, not only the blood flow in the myocardium but also that from the myocardium to the ventricular cavity, i.e., Thebesian venous blood flow, could be traced.

为了了解心内膜下缺血易感性的基础，我们使用带电荷耦合器件（CCD）相机的便携式针探针视频显微镜评估了心内膜下微血管的行为及其血流。针刺探针的尖端从跳动的犬心脏左心房插入左心室腔，观察心内膜下小动脉和小静脉。为了突出小血管图像，我们采用了：1)绿色滤光片（红色互补色），2)冠状动脉内注射fitc -葡聚糖，3)冠状动脉内或静脉注射吲哚菁绿。利用该技术可以很容易地识别动脉和静脉微血管，并评估其直径变化。我们评估了硝酸甘油对冠状动脉微血管的影响。研究表明硝酸甘油扩张心外膜下和心内膜下小动脉。硝酸甘油对心内膜下小动脉的血管扩张作用因血管大小而异。与直径小于100 μ m的小动脉相比，硝酸甘油使直径大于100 μ m的大动脉扩张。硝酸甘油增加心内膜下小动脉的脉动幅度。还发现硝酸甘油有扩张冠状静脉的倾向。硝酸甘油对心肌静脉血管的影响有待进一步研究。使用高对比度血流标记物——墨水，不仅可以追踪心肌内的血流，还可以追踪从心肌到心室腔的血流，即底比斯静脉血流量。