Development of 3-D Evaluation System of Microheterogeneity of Intramyocardial Flow Using Molecular Flow Tracers

使用分子流示踪剂开发心肌内流动微观不均匀性的 3D 评估系统

• 批准号: 08557051
• 负责人: GOTO Masami
• 金额: $ 4.48万
• 依托单位: Kawasaki College of Allied Health Professions
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557051/
• 关键词: myocardial ischemia; microcirculatory regulation; intramyocardial flow distribution; RI molecular tracer; heterogeneity; microflow-imaging; R1分子血流トレーサ

Growing experimental evidence support for the concept of high risk coronary microcirculatory unit that is at greatest risk of ischemia in the myocardium. However, there are many uncertainties of physiological and pathophysiological regulation of the microcirculatory blood flow in the myocardium. Therefore, in this project, to evaluate the anatomical features and functional characteristics of the intramyocardial microcirculatory unit, we aimed to develop a three-dimensional evaluation system of microheterogeneity of intramyocardial flow using molecular flow tracers. We measured within-layr distribution of regional myocardial flows in the left and right ventricles of anesthetized rabbits with myocardial region sizes in the range of 0.01-1.0 mm^2. A novel method of digital radiography combined with the technique of tritium-labeled desmethylimipramine deposition enabled us to visualize the myocardial flow distribution and to compute the coefficient of variation of regional myocardial flows. The coefficient of variation of regional flows of the right ventricle was higher than that of the left ventricle. In left ventricle, coefficient of variation of regional flows was higher in the subendocardium than subepicardium, while there was no significant difference between the two myocardial layrs of the right ventricle. Based on these results it was suggested that greater stress and its possible heterogeneity of the subendocardium of the left ventricle may contribute to the greater microheterogeneity of the within-subendocardial layr distribution of the nutritious flow.

越来越多的实验证据支持高风险冠状动脉微循环单元的概念，即心肌缺血的风险最大。然而，心肌微循环血流的生理和病理生理调节存在许多不确定性。因此，在本项目中，为了评价心肌内微循环单元的解剖学特征和功能特征，我们的目的是利用分子流示踪剂开发心肌内血流微异质性的三维评价系统。我们测量了麻醉家兔左、右心室局部心肌血流的层内分布，心肌区域大小在0.01-1.0 mm ^2之间。一种新的方法，结合数字化X线摄影技术的氚标记的去甲丙咪嗪沉积使我们能够可视化心肌血流分布和计算局部心肌血流的变异系数。右室局部血流变异系数大于左室。左心室内膜下心肌血流变异系数大于心外膜下心肌血流变异系数，而右心室两层心肌血流变异系数无显著性差异。根据这些结果，我们认为，更大的压力和它可能的异质性的左心室内膜下可能有助于更大的微观异质性的营养流的内膜下层内的分布。

项目成果

Toyota E,Songfang L,Kimura A,Hiramatsu O,Tachibana H,Chiba Y,Goto M,Ogasawara Y,Tsujioka K,Kajiya F: "Evaluation of intramyocardial coronary blood flow waveform during intraaortic balloon pumping in the absence or presence of coronary stenosis." Artificia

Toyota E,Songfang L,Kimura A,Hiramatsu O,Tachibana H,Chiba Y,Goto M,Ogasawara Y,Tsujioka K,Kajiya F：“在不存在或存在冠状动脉狭窄的情况下主动脉内球囊泵血期间心肌内冠状动脉血流波形的评估

Goto M, VanBavel Ed, et al: "Vasodilatory effect of pulsatile Pressure on coronary resistance vessels." Circ Res. 79. 1039-1045 (1996)

Goto M、VanBavel Ed 等人：“脉动压力对冠状动脉阻力血管的血管舒张作用。”

Morita K,Tsujioka K,Kimura A,Ogasawara Y,Goto M,Hiramatsu O,Kajiya F,Feigl EO: "a-Adrenergic vasoconstriction reduces systolic retrograde coronary blood flow." Am J Physiol. 273. H2746-H2755 (1997)

Morita K、Tsujioka K、Kimura A、Ogasawara Y、Goto M、Hiramatsu O、Kajiya F、Feigl EO：“α-肾上腺素能血管收缩可减少收缩期逆行冠脉血流。”

Matsumoto T他: "Myocardial blood flows in micro-regions of the rabbit right ventricle evaluated by a molecular flow tracer-in reference with the left ventricle." J Cardiovas Diag Proc. 13. 304 (1996)

Matsumoto T 等人：“通过左心室的分子流示踪剂评估兔子右心室微区域的心肌血流。”J Cardiovas Diag Proc。

Mochizuki S, Goto M, et all: "Direct measurement of nitric oxide in arterial wall using newly developed nitric oxide microsensor." Biomedical Engineering-Applications,Basis & Communications. 8. 18-23 (1996)

Mochizuki S、Goto M 等人：“使用新开发的一氧化氮微传感器直接测量动脉壁中的一氧化氮。”