Flowcytometric Analysis of Eosinophil Surface Antigens Utlizing

用IL-3、IL-5或GM-CSF孵育肝素化全血细胞后，观察到CD69的快速诱导和嗜酸性粒细胞表面CD11b的上调。CD69在模拟后2小时达到表达高峰，而CD11b在模拟后数小时达到表达高峰。在体内检测嗜酸性粒细胞膜抗原CD11b和CD69的表面表达。CD11b在外周血正常嗜酸性粒细胞上组成性表达。在正常或过敏患者（包括支气管哮喘和特应性皮炎）的循环嗜酸性粒细胞中几乎检测不到CD69表达。CD69在局部炎症部位的嗜酸性粒细胞中表达上调，如嗜酸性心炎的心包积液或变应性鼻炎的鼻分泌物。高嗜酸性粒细胞综合征的大量循环嗜酸性粒细胞在表面表达CD69，表明这些嗜酸性粒细胞最近因暴露于高浓度的嗜酸性细胞因子（如IL-3、IL-5和GM-CSF）而被激活。这些嗜酸性粒细胞表面抗原的快速诱导在以支气管哮喘和特应性皮炎为代表的嗜酸性粒细胞炎性病变的诱导过程中可能具有重要的功能。用超抗原诱导T细胞活化法分析新生儿T淋巴细胞活化和细胞因子产生的特点。新生儿和婴儿T淋巴细胞的抗原反应性和细胞因子谱可能与这一时期过敏性疾病的特征性病理有重要关系。新生儿CD4^+ T淋巴细胞在超抗原刺激下增殖旺盛。新生儿T细胞的增殖反应比成人记忆T细胞高得多，这是基于更多和更长时间的IL-2的产生，以及新生儿T细胞的Vbeta特异性T细胞增殖的选择性增殖。新生儿或幼稚T细胞对超抗原的抗原反应性增强可能与儿童期早期特征性的与脱屑性皮肤病变相关的发热性炎性疾病的发生密切相关。目前尚不清楚新生儿T细胞反应性的这些特征是否与儿童早期过敏性疾病的发病机制有关。少

Rapid induction of CD69 and upregulation of CD11b on eosinophil surface was observed after incubation of heparinized whole blood cells with IL-3, IL-5 or GM-CSF.Peak expression of CD69 was reached assoon as 2 hours after simulation, whereas CD11b expression reached the peak several hours after simulation. Surface expression of eosinophil membrane antigen CD11b and CD69 was also examined in vivo. CD11b is expressed constitutively on normal eosinophils in the peripheral blood. Little, if any CD69 expression was detectable on circulating eosinophils from normal or allergic patients, including bronchial asthma and atopic dermatitis. CD69 expression was upregulated on eosinophils from local inflammatory sites, such as pericardial efusion of eosinophilic carditis, or nasal discharge from allergic rhinitis.A significant proportion of circulating eosinophils from hypereosinophilic syndrome expressed CD69 on the surface, indicating that these eosinophils are recently activated by exposure to co … More ncentrated eosinophilopoietic cytokine, such as IL-3, IL-5 and GM-CSF.Tese rapid induction of eosinophil surface antigens may be functionally significant during the induction of eosinophilic inflammatory lesions as represented by bronchial asthma and atopic dermatitis.Characteristics of neonatal T lymphocyte activation and cytokine production was analyzed by superantigeninduced T cell activation.Antigen-responsiveness and cytokine profiles of neonatal and infantile T lymphocytes may have important relatiionship with the characteristic pathology of allergic disorders during this period of life. Neonatal CD4^+ T lymphocyte proliferated vigorously to superatigen stimulation. The much higher proliferative resopnses of neonatal T cells than adult memory T cells was shown to be based on the greater and prolonged production of IL-2, together with the selective proliferation of Vbeta specific T cell proliferation of neonatal T cells. The enhance antigen responsiveness of neonatal or naive T cells to superantigen may be closely related to the occurrence of febrile inflammatory illness associated with desquamative skin lesions, characteristic during early childhood. It is not known if these characteristics of neonatal T cell responsiveness is related to the pathogenesis of allergic disorders during early childhood. Less