Flowcytometric Analysis of Eosinophil Surface Antigens Utlizing

利用流式细胞术分析嗜酸性粒细胞表面抗原

• 批准号: 04670583
• 负责人: YACHIE Akihiro
• 金额: $ 1.41万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670583/
• 关键词: Eosinophil; CD69; CD11b; Cytokines; Superantigens; TCRVβ; 表面抗原; フロー・サイトメトリー; 全血法; アレルギー疾患

Rapid induction of CD69 and upregulation of CD11b on eosinophil surface was observed after incubation of heparinized whole blood cells with IL-3, IL-5 or GM-CSF.Peak expression of CD69 was reached assoon as 2 hours after simulation, whereas CD11b expression reached the peak several hours after simulation. Surface expression of eosinophil membrane antigen CD11b and CD69 was also examined in vivo. CD11b is expressed constitutively on normal eosinophils in the peripheral blood. Little, if any CD69 expression was detectable on circulating eosinophils from normal or allergic patients, including bronchial asthma and atopic dermatitis. CD69 expression was upregulated on eosinophils from local inflammatory sites, such as pericardial efusion of eosinophilic carditis, or nasal discharge from allergic rhinitis.A significant proportion of circulating eosinophils from hypereosinophilic syndrome expressed CD69 on the surface, indicating that these eosinophils are recently activated by exposure to co … More ncentrated eosinophilopoietic cytokine, such as IL-3, IL-5 and GM-CSF.Tese rapid induction of eosinophil surface antigens may be functionally significant during the induction of eosinophilic inflammatory lesions as represented by bronchial asthma and atopic dermatitis.Characteristics of neonatal T lymphocyte activation and cytokine production was analyzed by superantigeninduced T cell activation.Antigen-responsiveness and cytokine profiles of neonatal and infantile T lymphocytes may have important relatiionship with the characteristic pathology of allergic disorders during this period of life. Neonatal CD4^+ T lymphocyte proliferated vigorously to superatigen stimulation. The much higher proliferative resopnses of neonatal T cells than adult memory T cells was shown to be based on the greater and prolonged production of IL-2, together with the selective proliferation of Vbeta specific T cell proliferation of neonatal T cells. The enhance antigen responsiveness of neonatal or naive T cells to superantigen may be closely related to the occurrence of febrile inflammatory illness associated with desquamative skin lesions, characteristic during early childhood. It is not known if these characteristics of neonatal T cell responsiveness is related to the pathogenesis of allergic disorders during early childhood. Less

IL-3、IL-5和GM-CSF刺激后，嗜酸性粒细胞表面CD 69和CD 11b表达均迅速增加，刺激后2 h达高峰，刺激后数小时达高峰。还在体内检测了嗜酸性粒细胞膜抗原CD 11b和CD 69的表面表达。CD 11b在外周血中的正常嗜酸性粒细胞上组成性表达。在正常或过敏患者（包括支气管哮喘和特应性皮炎）的循环嗜酸性粒细胞上几乎检测不到CD 69表达（如果有的话）。CD 69在局部炎症部位的嗜酸性粒细胞上表达上调，如嗜酸性粒细胞性心脏炎的心包灌流，或过敏性鼻炎的鼻分泌物。 ...更多信息 浓缩的嗜酸性粒细胞生成细胞因子，如IL-3，嗜酸性粒细胞表面抗原的快速诱导在以支气管哮喘和特应性皮炎为代表的嗜酸性粒细胞炎性病变的诱导过程中可能具有重要的功能意义。通过超抗原诱导的T细胞活化来分析新生儿T淋巴细胞活化和细胞因子产生的特征。抗原-新生儿和婴儿T淋巴细胞的反应性和细胞因子谱可能与这一生命时期变态反应性疾病的特征性病理学有重要关系。新生儿CD 4 ^+ T淋巴细胞在超抗原刺激下增殖旺盛。新生儿T细胞比成人记忆T细胞高得多的增殖反应显示是基于IL-2的更大和延长的产生，以及新生儿T细胞的Vbeta特异性T细胞增殖的选择性增殖。新生儿或幼稚T细胞对超抗原的抗原反应性增强可能与儿童早期特有的与脱屑性皮肤病变相关的发热性炎症性疾病的发生密切相关。目前尚不清楚新生儿T细胞反应性的这些特征是否与儿童早期过敏性疾病的发病机制有关。少

项目成果

谷内江 昭宏: "メモリー細胞" 内科. 71. 1307-1307 (1993)

Akihiro Yauchie：“记忆细胞”内科。 71. 1307-1307 (1993)

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Tsuji, T.、Nibu, R.、Iwai, K.、Kanegane, H.、Yachie, A.、Seki, H.、Miyawaki, T.、Taniguchi, N.：“有效诱导新生儿幼稚 B 中免疫球蛋白的产生

A.Yachie et al: "Defective production of interleukinー6 in very small premature infants in response to bacterial pathogens." Infection and Imrunnity. 60. 7449-7453 (1992)

A. Yachie 等人：“非常小的早产儿因细菌病原体而产生白细胞介素 6 缺陷。” 60. 7449-7453 (1992)。

Yachie, A., Takano, N., Ohta, K., Uehara, T., Fujita, S., Miyawaki, T.and Taniguchi, N.: "Defective production of interleukin-6 in very small premature infants in response to bacterial pathogens" Infact. Immun.60. 749-753 (1992)

Yachie, A.、Takano, N.、Ohta, K.、Uehara, T.、Fujita, S.、Miyawaki, T. 和 Taniguchi, N.：“非常小的早产儿中白细胞介素 6 的产生缺陷，是由于

Morise, T., Takeuchi, Y., Takeda, R., Karayalcin, U., Yachie, A., Miyawaki, T.: "Increased plasma endothelin levels in Kawasaki disease : a possible marketfor kawasaki disease" J.vasc. Dis. Sep.719-723 (1993)

Morise, T.、Takeuchi, Y.、Takeda, R.、Karayalcin, U.、Yachie, A.、Miyawaki, T.：“川崎病血浆内皮素水平升高：川崎病的可能市场”J.vasc。