IGE BINDING AND CD38 EXPRESSION ON BASOPHIL SURFACE DURING INFANCY AND THEIR FUNCTIONAL RELEVANCE

婴儿期嗜碱性粒细胞表面的 IGE 结合和 CD38 表达及其功能相关性

基本信息

  • 批准号:
    07670847
  • 负责人:
  • 金额:
    $ 1.47万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

Peripheral blood basophils express FcepsilonRI on the surface. The regulatory mechanism of its expression is, however, largely unknown. In particular, there has been no report regarding the FcepsilonRI expression on circulating basophils during childhood. In this study, we evaluated IgE binding and FcepsilonRI expression on basophil surface in neonates and in children of various age groups. Furthermore, the regulation of FcepsilonRI by monomeric IgE was examined. In addition, serum IgE concentration was determined by a sensitive ELISA assay using anti-IgE monoclonal antibodies. Basophil-bound IgE was significantly high among allergics than normal controls. The difference was more prominent among younger age groups. The surface-bound IgE correlated very well with serum IgE concentrations. The level increased rapidly around 100 ng/ml of IgE and it reached a plateau when serum IgE was higher than 300 ng/ml. IgE-binding cell was undetectable within neonatal cord blood. However, IgE binding was induced rapidly when the cells were incubated with excess monomeric IgE for short period. Longer incubation resulted in further potentiation of IgE binding. FcepsilonRI expression on basophil surface also correlated well with serum IgE.FcepsilonRI expression on neonatal basophils was relatively low, but the level was upregulated when these cells were incubated with IgE.The enhancement was dependent on de novo protein synthesis. Constitutive synthesis of FcepsilonRI mRNA synthesis was observed in neonatal blood and the level was not significantly increased after the addition of IgE,indicating that a mechanism other than mRNA transcription is involved in the regulation of surface IgE receptor expression.
外周血嗜碱性粒细胞表面表达FcepsilonRI。然而,其表达的调控机制在很大程度上是未知的。特别是,还没有关于FcepsilonRI在儿童时期循环中的嗜碱性粒细胞上表达的报道。在这项研究中,我们评估了新生儿和不同年龄段的儿童嗜碱性粒细胞表面的IgE结合和FcepsilonRI的表达。此外,还检测了单体IgE对FcepsilonRI的调节作用。此外,用抗IgE单抗建立了一种灵敏的酶联免疫吸附试验测定血清IgE浓度。变态反应组中嗜碱粒细胞结合的IgE显著高于正常对照组。这一差异在较年轻的年龄组中更为突出。表面结合的IgE与血清IgE浓度有很好的相关性。血清Ig E水平在100 ng/m l左右迅速升高,当血清Ig E>300 ng/m l时达到平台期,新生儿脐血中未检测到Ige结合细胞。然而,当细胞与过量的单体IgE孵育较短时间时,可迅速诱导其与IgE结合。随着孵育时间的延长,IgE结合能力进一步增强。嗜碱性粒细胞表面FcepsilonRI的表达与血清IgE水平也有很好的相关性,新生儿嗜碱性粒细胞表面FcepsilonRI的表达相对较低,但与IgE共同孵育后,其表达水平上调,这种上调依赖于新生蛋白的合成。在新生儿外周血中观察到FcepsilonRI mRNA合成的组成性合成,加入IgE后其合成水平没有明显增加,表明表面IgE受体表达的调节可能是通过转录以外的机制来实现的。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kanegane H et al: "Expression of L-selectin (CDb2L)discriminates The-and The-like cipokine-producing memury CD4^+ T cells." Inumunology. 87. 186-190 (1996)
Kanegane H 等人:“L-选择素 (CDb2L) 的表达可区分产生类似 cipokine 的记忆 CD4^ T 细胞。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
A. Yachie et al: "Delineation of Producing abiliby of IgG and IgA subclasses by naive B cells in newborn infants and adult individuals" Clin. Exp. Immunol.102. 204-209 (1995)
A. Yachie 等人:“新生婴儿和成人个体中幼稚 B 细胞产生 IgG 和 IgA 亚类能力的描述”Clin。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
東馬智子他: "アレルギー疾患にみられる好塩基球IgE結合状態のフローサイトメトリー法による評価" アレルギー. 45 (7). 627-636 (1996)
Tomoko Touma 等人:“通过流式细胞术评估过敏性疾病中观察到的嗜碱性粒细胞 IgE 结合状态”,过敏症 45 (7)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kawano M et al: "Absence of CD69 expression on peripheral eosinophilia in episodic angioldema and eosinophilia." Am. J. Hematology. 53. 43-45 (1996)
Kawano M 等人:“阵发性血管性水肿和嗜酸性粒细胞增多症中外周嗜酸性粒细胞增多症中不存在 CD69 表达。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshikawa H et al: "Degos′s disease : radiological and immunological aspects." Acta Neurol. Scand.94. 353-356 (1996)
Yoshikawa H 等人:“德戈斯病:放射学和免疫学方面。”Acta Neurol.94(1996)。
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  • 影响因子:
    0
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YACHIE Akihiro其他文献

YACHIE Akihiro的其他文献

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{{ truncateString('YACHIE Akihiro', 18)}}的其他基金

Establishment of novel diagnostic parameters for the early therapeutic intervention of EBV-associated lymphoproliferative diseases.
为 EBV 相关淋巴增殖性疾病的早期治疗干预建立新的诊断参数。
  • 批准号:
    21591353
  • 财政年份:
    2009
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanisms underlying the complex clinical features of combined immunodeficiency disorders
联合免疫缺陷疾病复杂临床特征的分子机制
  • 批准号:
    19591244
  • 财政年份:
    2007
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of novel anti-inflammatory therapy through regulation of heme oxygenase-1 production by monocytes
通过调节单核细胞血红素加氧酶-1 的产生开发新型抗炎疗法
  • 批准号:
    16591014
  • 财政年份:
    2004
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research on regulatory mechanism of inflammation by HO-1 and development of novel anti-inflammatory threrapy
HO-1炎症调节机制研究及新型抗炎治疗药物开发
  • 批准号:
    14570729
  • 财政年份:
    2002
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
MECHANISM OF ALLERGIC SENSITIZATION DURING INFANCY
婴儿期过敏致敏机制
  • 批准号:
    09670792
  • 财政年份:
    1997
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Flowcytometric Analysis of Eosinophil Surface Antigens Utlizing
利用流式细胞术分析嗜酸性粒细胞表面抗原
  • 批准号:
    04670583
  • 财政年份:
    1992
  • 资助金额:
    $ 1.47万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似国自然基金

过敏煎通过调控“Basophil-MC”轴缓解特应性瘙痒的作用机制研究
  • 批准号:
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