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Predictive assay for late radiation injury with fibroblast and lymphocyte

成纤维细胞和淋巴细胞晚期放射损伤的预测分析

基本信息

批准号：
04670677
负责人：
ITO Hisao
金额：
$ 1.34万
依托单位：
School of Medicine, Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Radiotherapy is very useful treatment modality for cancer patients. However, there are several problems to perform clinical radiotherapy. Those are (1) it is very difficult to prospect radiosensitivity of each cancer before radiotherapy, and (2) it is impossible to predict late radiation injury before it happens. This study was performed to develop the predictive assay method to detect late radiation damage and radio-sensitivity of cancer before radiotherapy. Patients with cervical cancer were taken the cancer tissue, the skin subcutaneous tissue and lymphocytes. Cultured cells were established from these tissues. They were irradiated and radiosensitivities for each cell strain were determined. The survival curves were made with densitometry assay method for cancer cells, and with ordinary colony assay method for fibroblasts and lymphocytes. The growth rates of fibroblast were different in each strain and it was very difficult to establish the useful assay method. For cancer cells, the densitometry assay showed slightly higher survival rates than the colony assay, but there was good correlation between these two methods. On the other hand, the relationship between radiosensitivities of fibroblasts and lymphocytes and late radiation injury was not determined. The reasons were (1) number of successful cultures was short, and (2) an incidence of late radiation injury was small number. We concluded that it is important to develop the more easy and sensitive assay method, for example, measurement of some factors in the serum. In future, the developments of molecular analyzes of radiation damage can provide the some better assay methods.

放射治疗是癌症病人非常有用的治疗方式。然而，进行临床放射治疗存在一些问题。这两点是：（1）在放射治疗前很难对每种肿瘤的放射敏感性进行前景预测;（2）在放射损伤发生之前无法预测晚期放射损伤。本研究旨在建立一种放射治疗前预测肿瘤放射敏感性和晚期放射损伤的方法。对宫颈癌患者分别取癌组织、皮肤皮下组织及淋巴细胞。从这些组织建立培养细胞。对它们进行辐照，并测定每种细胞株的放射敏感性。癌细胞用光密度测定法作存活曲线，成纤维细胞和淋巴细胞用普通集落测定法作存活曲线。各菌株成纤维细胞的生长速度不同，建立实用的检测方法十分困难。对于癌细胞，光密度测定法显示存活率略高于殖民地测定法，但这两种方法之间有很好的相关性。另一方面，成纤维细胞和淋巴细胞的辐射敏感性与晚期辐射损伤之间的关系尚未确定。其原因是（1）培养成功的数量少，（2）晚期放射损伤的发生率少。因此，发展更简便、灵敏的检测方法，如血清中某些因子的测定，是十分重要的。未来辐射损伤分子分析技术的发展，将为辐射损伤的检测提供更好的方法。