Analysis of Redioresistant Tumor by Heavy Particle Irradiation

重粒子线照射分析抗放射肿瘤

• 批准号: 06670936
• 负责人: ITO Hisao
• 金额: $ 1.34万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670936/
• 关键词: Carbon beamd; Neon beams; LET; Mutation; Radiotherapy; hprt; 放射線療法; 重粒子線; 培養細胞; 放射線感受性

The tumors are sometimes resistant to radiotherapy or regrow after regression by radiotherapy. These tumors are expected to have residual viable tumor cells in the tumor and easily regrow the tumor cells. This phenomenon may depend on the higher of repair of the damaged DNA or accelerated capability facility of the residual tumor cell. There are several differences of biological responses between tumor cells exposed to the X-rays and to the heavy particles. This phenomenon is expressed as relative biological effectiveness (RBE). When the tumor cells are irradiated with heavy particles of same LET and same doses, RBEs depend on each tumor cell line. This study was performed to develop the method to estimate the radiosensitivity of the tumor cell to X-ray irradiation from the radiosensitivity to heavy particles. In this study, 8 in cell lines those are established from adenocarcinoma of the ovarian cancer or cervical cancer, 6 cell lines established from squamous cell carcinoma of the es … More ophageal cancer and one from malignant melanoma were irradiated with X-rays. The radiosensitivity was determined by the colony forming assay. The cells established from human tumors were different from those from the animal tissues. The former cells had smaller Do and n values than the latter, and showed steeper cell survival curves. There was no significant difference between cell lines from adenocarcinoma and those from squamous cell carcinoma. When the cells were irradiated with carbon beams or neon beams, they showed the same survival curves for the same LET and same doses. The mutation of DNA that was induced by heavy particle irradiation was determined by the expression of hypoxanthine-guanine phosphrybosyl transferase (hprt) locus. The frequency of expression of hprt locus was completely different between carbon beam irradiation and neon beams. There was no significant difference of radiosensitivity between carbon beams and neon beams, but carbon beams induced higher mutation than neon beams. This result suggests that neon beams may be useful in clinical radiotherapy. Less

肿瘤有时对放射治疗有抵抗性，或在放射治疗消退后再生长。这些肿瘤有望在肿瘤中残留有活的肿瘤细胞，并且肿瘤细胞很容易再生。这种现象可能与受损DNA的修复能力较高或残留肿瘤细胞的加速能力有关。肿瘤细胞暴露在x射线和重粒子下的生物反应有几个不同。这种现象被表示为相对生物有效性（RBE）。当肿瘤细胞用相同LET和相同剂量的重粒子照射时，RBEs取决于每个肿瘤细胞系。本研究旨在从重粒子辐射敏感性出发，建立肿瘤细胞对x射线辐射敏感性的评估方法。在本研究中，8个来自卵巢癌腺癌或宫颈癌的细胞系，6个来自食管鳞状细胞癌的细胞系被x射线照射，更多的食管癌和1个来自恶性黑色素瘤。用菌落形成试验测定放射敏感性。从人类肿瘤组织中建立的细胞与从动物组织中建立的细胞不同。前者细胞的Do和n值小于后者，细胞存活曲线更陡。腺癌细胞系与鳞状细胞癌细胞系间无明显差异。当细胞用碳束或氖束照射时，它们在相同的LET和相同的剂量下显示出相同的存活曲线。重粒子辐照诱导的DNA突变是由次黄嘌呤-鸟嘌呤磷酸基转移酶（hprt）位点的表达决定的。hprt基因座的表达频率在碳束和霓虹灯照射下完全不同。碳束和氖束的辐照敏感性无显著差异，但碳束诱导的突变高于氖束。这一结果提示霓虹灯在临床放射治疗中可能是有用的。少

项目成果

Kazuhiko Toya: "Analysis of mutation induction for cultured cells irradiated with heavy ion beams and X-ray irradiations" Nippon Acta Radiologica. (in Press).

洞谷一彦：“重离子束和X射线照射培养细胞的突变诱导分析”日本放射线学报。

戸矢和仁: "X線および重粒子線照射による遺伝子突然変異発生頻度の定量-ヒト培養細胞における-" 日本医学放射線学会誌. (印刷中).

Kazuhito Toya：“通过 X 射线和重离子照射量化基因突变频率 - 在培养的人体细胞中 -”日本医学放射学会杂志（正在出版）。

Wei-Jei Ka: "Relative Biological Eeffect and Mutation of Human Tumor cells Irradiated with Heavy Particles" Nippon Acta Radiologica. (in Press).

Wei-Jei Ka：“用重粒子照射的人类肿瘤细胞的相对生物学效应和突变”Nippon Acta Radiologica。

柯,偉傑: "重粒子線照射によるヒト培養細胞のRelative Biological EffectとMutation" 日本医学放射線学会誌. (印刷中).

柯伟杰：“重离子照射对培养的人体细胞的相对生物学效应和突变”，日本医学放射学会杂志（出版中）。