权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Application of Modified FISH to Analyze Tumor Cell Radioresistance and Development of Its Clinical Application

改良FISH分析肿瘤细胞放射抗性及其临床应用进展

基本信息

批准号：
11670869
负责人：
ITO Hisao
金额：
$ 2.18万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670869/
关键词：
FISH method Transformation Radiosensitivity Calicurein A Lymphocyte カリクレイン

项目摘要

In the conventional chromosome analysis, the cells in the M-phase can be the target. However, the efficiency of this method was very low and sufficient results could not be expected. When the transformation of the chromosome was adapted as the index, the sensitivity of this method was also low. As a result, analysis of chromosome aberratoin had been a tough business. The condensed chromoses were stained with fluorescent dyes for the specific chromosome in this study instead of the conventional method. The radiosensitivity of the culture cells established from bladder cancer, ovarian cancer, uterine neck cancer, esophageal cancer were examined by the Colony assay in 1999, and basic data for radiosensitivity of the cells were obtained. As a results, the culture cells of which differed in the Do value of about the double radiosensitivity were clarified. Chromosomal aberration in irradiated lymphocytes was examined in 2000. The lymphocytes were collected at each exposure radiation dose(2-12 Gy)from the patient with the whole body irradiation. The relationship between chromosomal aberration and exposure radiation dose was analyzed with chromosome analyses and FISH at chromosome 1, 2. As The result, there was good correlation between the chromosome aberrations and irradiation doses. When the lymphocytes collected from the same patient were irradiated in in vitro, the same correlation between chromosome aberrations and irradiation doses was found as the in vivo analyses. This result suggests that chromosome aberration of lymphocytes analysed in vitro with FISH at chromosome 1, 2 has a good correlation with that in vivo at the doses between 2-12 Gy. In the analyses of chromosome aberration of the tumor cells, there were some much transformations before the irradiation. As a result, it was difficult to evaluate the effect of irradiation. This study will be continued with tumor cells in the future.

在常规染色体分析中，处于M期的细胞可以是目标。然而，这种方法的效率非常低，不能期望得到足够的结果。当以染色体的转化率为指标时，该方法的灵敏度也较低。因此，染色体畸变的分析一直是一项坚韧的任务。本研究用荧光染料代替常规染色方法，对浓缩的染色体进行染色。1999年用殖民地法检测了膀胱癌、卵巢癌、宫颈癌、食管癌的培养细胞的放射敏感性，获得了细胞放射敏感性的基本数据。结果，澄清了其Do值不同的培养细胞，约为两倍放射敏感性。2000年检查了受辐射淋巴细胞的染色体畸变。在每次照射剂量（2-12戈伊）下，从全身照射的患者中收集淋巴细胞。采用染色体分析和1、2号染色体荧光原位杂交技术，分析染色体畸变与辐射剂量的关系。结果表明，染色体畸变与照射剂量之间有良好的相关性.当从同一病人收集的淋巴细胞在体外照射，染色体畸变和辐射剂量之间的相关性被发现在体内分析相同。结果表明，在2-12戈伊剂量范围内，用FISH技术分析的淋巴细胞1、2号染色体畸变与体内试验结果有较好的相关性。在肿瘤细胞染色体畸变分析中，发现照射前肿瘤细胞染色体畸变发生了较大的变化。因此，很难评价辐照的效果。这项研究将在未来继续与肿瘤细胞。