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Analysis of Ig Gene in Indolent Malignant Lymphoma and its Application to the Therapy

惰性恶性淋巴瘤Ig基因分析及其在治疗中的应用

基本信息

批准号：
09670918
负责人：
ITO Hisao
金额：
$ 2.05万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670918/
关键词：
Malignant lymphoma DNA analysis Immunoglobulion follicular lymphoma transformation ろほう性リンパ腫

项目摘要

Malignant lymphoma cells which derived from the same clonal cell have the same region on the Ig gene, even if the histologic features are changed. For example, follicular lymphoma progressed to the more aggressive lymphoma, same region on the Ia gene can be detected in the both malignant cells, This study was performed to determine the relationship between resistance to therapies and the arrangement of bases of DNA.Furthermore, we intended to improve the treatment outcome applying the results of this study. At the first step, the clinical results (by BACOD-E therapy) were analyzed to detect the cases those were resistant to the therapy. The International Prognosis Index (IPI) and values of LDH were not predictors of the prognosis in this study. One case who developed myelodysplasia syndrome followed by acute myelogenous leukemia, showed chromosome translocation, t(11 ; 19)(q23 ; p13). We further analyzed the other patients including the previous ones. Two hundreds forty four patients were reanalyzed according to Revised European and American Classification for Lymphoid Neoplasms (REAL classification). The incidence of follicle center lymphoma (FCL) was 4.4%, and the most common disease was diffuse large B-cell lymphoma (37%). In this study, IPI was the useful prognostic factor. As the experimental study, lymphoma cells were extracted from the Paraffin-fixed specimen under the microscope. DNA was extracted from these cells and proliferated with PCR.The Ig genes were analyzed for each DNA samples to correlate the histological features. However, until now, there were not any positive relationship between Ig gene analyses and histology or resistance to the therapies.

来自同一克隆细胞的恶性淋巴瘤细胞在免疫球蛋白基因上具有相同的区域，即使组织学特征发生了变化。例如，滤泡性淋巴瘤进展为更具侵袭性的淋巴瘤，在这两种恶性细胞中都可以检测到Ia基因上的相同区域，本研究旨在确定DNA碱基排列与耐药的关系，并试图应用本研究的结果来改善治疗结果。第一步，对BACOD-E治疗的临床结果进行分析，以发现对该治疗耐药的病例。在本研究中，国际预后指数(IPI)和乳酸脱氢酶(LDH)值不能预测预后。1例骨髓增生异常综合征继发急性髓系白血病，染色体易位，t(11；19)(q23；p13)。我们进一步分析了其他患者，包括以前的患者。根据修订的欧美淋巴肿瘤分类(REAL分类)对244例患者进行再分析。滤泡中心淋巴瘤(FCL)的发病率为4.4%，最常见的疾病是弥漫性大B细胞淋巴瘤(37%)。在本研究中，IPI是有用的预后因素。作为实验研究，在显微镜下从石蜡包埋标本中提取淋巴瘤细胞。从这些细胞中提取DNA，与PCR一起增殖，分析每个DNA样本的Ig基因，以关联组织学特征。然而，到目前为止，免疫球蛋白基因分析与组织学或治疗耐药性之间没有任何正相关关系。