A study of frequency inductive heating using liposomal

脂质体频率感应加热的研究

• 批准号: 04670751
• 负责人: ARAI Kazushige
• 金额: $ 1.34万
• 依托单位: SHOWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670751/
• 关键词: Hyperthermia; HIgh frequency inductive heating; Gastrointestial cancer; Ferromagnetic particle; 高周波誘導加温; ハイパーサーミア; リポソーム

As for hyperthermia in Japan, cases of capasitive heating using RF wave presently account for the majority, but selective heating is difficukt and there still is a room for study as to hyperthremia for gastrointestinal cancers which are located deep in the abdominal cavity. In the present study we prepared liposomal ferromagnetic particles (hereinafter refered to as HP-LM) by cvering Fe_3O_4 particles of which mean particle size is 15 nm with lipid molecular membrane of liposome and having hem atoporphyrin, the purpose of developing a selective heating method for deep tumors. Furthermore, VX-2 tumor cells were implantd in the lower legs of rabbit, and HP-LM was administered intraarterialy, andhigh frequency inductive heating was done. The intraarterial administration group, as compared with the nonadministration group, showed a significant rise in temperature in tumors as wellas the tumor-growth-inhibiting effects. However, repeated administration of HP-LM was considered necessary toprevent the growth of tumorsfor a long period of time. Therefore, high frequency inductive heatingwas done by administrating HP-LM repeatedlyfrom a reservoir for intraarerial injection, with the tumor grown 4.2 times after 21 days in the nonadiministration and nonheating group, and 1.7 tines after 21 days in the repeated administration and heating group. When the tumor was ecamined histologically, magnetic particles were accumulated in the tumor tissue. However. accumulation in the reticuloendot helial organs was also high, so a study on the metabolic route is the subject of future study.

在日本，目前采用射频热疗的病例占多数，但选择性热疗困难，对位于腹膜深处的胃肠道肿瘤的热疗尚有研究空间。本研究通过将平均粒径为15 nm的Fe_3O_4颗粒与脂质体的脂质分子膜结合，制备出平均粒径为15 nm的Fe_3O_4脂质体铁磁性颗粒(以下简称HP-LM)，旨在开发一种治疗深部肿瘤的选择性加热方法。在此基础上，将VX-2肿瘤细胞接种于兔小腿，动脉内注射HP-LM，并进行高频感应加热。动脉内给药组与非给药组相比，肿瘤温度显著升高，肿瘤生长受到抑制。然而，反复给药被认为是必要的，以防止肿瘤生长较长一段时间。因此，采用高频感应加热的方法，多次从储藏室内注射HP-LM进行动脉注射，21天后肿瘤生长4.2倍，重复给药加热组21天后肿瘤生长1.7倍。当肿瘤组织学检查时，肿瘤组织中可见磁性颗粒聚集。然而。网突螺旋器官中的积累量也很高，因此对代谢途径的研究是未来研究的主题。

项目成果

上地一平・鈴木恵史・新井一成・新井浩士・小池正・小口勝司: "リポソーム性強磁性体微粒子による高周波誘導加温法の研究-家兎加温実験による腫瘍の縮小率について-" 昭和医学会雑誌. 53. 182-188 (1993)

Kamichi Ippei、Keiji Suzuki、Kazunari Arai、Hiroshi Arai、Tadashi Koike 和 Katsuji Oguchi：“使用脂质体铁磁颗粒的高频感应加热方法的研究 - 关于兔子加热实验的肿瘤缩小率 -”昭和医学杂志社会。53。182-188（1993）

S.Suzuki, K.Arai, H.Kushiro, M.Fukushima, M.Murakami, T.Koike, J.Ishii, et al.: "Sutudies on liposomal ferromagnetic particles and a technique of high frequency inductive heating (Report *)" Jpn. Soc. Hyperthermic Oncol.236-238 (1988)

S.Suzuki、K.Arai、H.Kushiro、M.Fukushima、M.Murakami、T.Koike、J.Ishii 等人：“脂质体铁磁颗粒和高频感应加热技术的研究（报告*）

S.Suzuki, K.Arai, T.Koike and K.Oguchi: "Sutudies on liposomal ferromagnetic particles and a technique of high frequency inductive heating -IN vivo studies of rabbits-" J.Jpn. Soc. Canver Ther.25(11). 2649-2658 (1990)

S.Suzuki、K.Arai、T.Koike 和 K.Oguchi：“脂质体铁磁颗粒的研究和高频感应加热技术 - 兔子的体内研究 -” J.Jpn。

M.Okazaki, M.Tuji, K.Oguchi, H.Arai, I.kamichi, S.Suzuki, K.Arai and T.Koike: "A fundamental study of preparation of loposomes containing ferromagnetic particles" Showa Univ. J.Med. Sci.3(1). 1-9 (1991)

M.Okazaki、M.Tuji、K.Oguchi、H.Arai、I.kamichi、S.Suzuki、K.Arai 和 T.Koike：“含铁磁性颗粒的脂质体制备的基础研究”昭和大学。

S.Suzuki, K.Arai, H.Arai, M.Nakanishi, H.Kushiro, M.Fukushima, M.Murakami, T.Koike and K.Oguchi: "Sutudies on liposomal ferromagnetic particles and a technique of high frequency inductive heating (Report *)" Jpn. Soc. Hyperthermic Oncol.402-403 (1989)

S.Suzuki、K.Arai、H.Arai、M.Nakanishi、H.Kushiro、M.Fukushima、M.Murakami、T.Koike 和 K.Oguchi：“脂质体铁磁颗粒和高频感应加热技术的研究”