STUDIES ON LIPOSOMAL FERROMAGNETIC PARTICLES AND A TECHNIQUE OF HIGH FREQUENCY INDUCTIVE HEATING

在将铁磁颗粒施用到肿瘤中之后，可以通过施加高频感应加热来更选择性地加热肿瘤。为了设计一种利用高频感应加热对肿瘤进行选择性热疗的技术，我们继续使用脂质体铁磁颗粒（HP-LM）进行基础研究，其制备如下：（Fe_3O_4）微粒表面包被含有血卟啉的脂质体膜，血卟啉具有肿瘤亲和性。我们使用高频感应加热器（日本KOSHUHA CO. LTD. YKN-10），频率：400 KHz，输出：10 KW，线圈直径：90 mm。将VX-2肿瘤细胞以1.5 × 10^7接种于兔大腿下部。A、B、C三组分别于股动脉内注入HP-LM 200 mg/kg、Fe_3O_4 100 mg/kg、Fe_3O_4 1 关于我们 A组：HP-LM 200 mg/kg，加热10分钟，使肿瘤中心温度升高14.8 ℃; B组：Fe_3O_4 100 mg/kg，加温10分钟，使肿瘤中心温度升高10.6 ℃。C组：结扎股动脉，加热10分钟，肿瘤中心温度升高6.5 ℃。D组：不给药，加热10分钟，肿瘤中心温度升高4.4 ℃。A组的体温升高明显高于另一组。A组组织学特征为肿瘤中心和边缘的毛细血管及肿瘤细胞内可见铁颗粒染色，认为该技术是非常有用的治疗方法，因为HP-LM可通过高频感应加热获得选择性热疗，这是HP-LM吸附于肿瘤组织的结果。少

The tumor can be more selectively heated by applying of high frequency inductive heating after administration of ferromagnetic particles into a tumor. In order to devise a technique used for the selective hyperthermia of a tumor with high frequency inductive heating, we continued fundamental studies using liposomal ferromagnetic particles (HP-LM), which were prepared as follows: triiron tetoraoxide (Fe_3 O_4) particles were coated with liposomal membrane contained hematoporphyrin with neoplastic affinity.In Vivo Studies of Rabbits: We used high frequency inductive heater (NIHON KOSHUHA CO. LTD. YKN-10), frequency: 4OOKHz, out put: 1OKw, coil diameter: 90mm. VX-2 tumor cells at 1.5X10^7 were implanted to the lower thigh of rabbit. When the implanted tumor grew up 3cm in diameter, A-D group were heated and studied.Group A: In a dose of 200mg/kg of HP-LM was administrated into the femoral artery, Group B: In a dose of 100mg/kg of Fe_3 O_4 was administrated into the femoral artery, Group C … More : Ligation of femoral artery, Group D: Nonadministrated, and then was immediately heated.Group A: In a dose of 200mg/kg of HP-LM, with heating for 10 minutes, temperature was elevated 14.8ﾟC at center of tumor. Group B: In a dose of 100mg/kg of Fe_3 O_4, with heating for 10 minutes, temperature was elevated 10.6ﾟC at center of tumor. Group C: In a ligation of femoral artery, with heating for 10 minutes, temperature was elevated 6.5ﾟC at center of tumor. Group D: In nonadministration, with heating for 10 minutes, temperature was elevated 4.4ﾟC at center of tumor. Temperature elevation of Group A was significantly higher in comparison with those found in another group. In histological features in Group A, iron particles were stained in blood capillaries at center and edge of tumors, and in tumor cells.It was thought that this technique is very useful treatment, because selective hyperthermia could be obtained by high frequency inductive heating of HP-LM, as a result of adsorption of HP-LM to tumor tissue. Less