STUDIES ON LIPOSOMAL FERROMAGNETIC PARTICLES AND A TECHNIQUE OF HIGH FREQUENCY INDUCTIVE HEATING

脂质体铁磁颗粒及高频感应加热技术的研究

• 批准号: 63570613
• 负责人: ARAI Kazushige
• 金额: $ 1.34万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570613/
• 关键词: HYPERTHERMIA; HIGH FREQUENCY INDUCTIVE HEATING; FERROMAGNETIC PARTICLE; LYPOSOME; 腫瘍選択的加温; ハイパーサーミア; リポソーム

The tumor can be more selectively heated by applying of high frequency inductive heating after administration of ferromagnetic particles into a tumor. In order to devise a technique used for the selective hyperthermia of a tumor with high frequency inductive heating, we continued fundamental studies using liposomal ferromagnetic particles (HP-LM), which were prepared as follows: triiron tetoraoxide (Fe_3 O_4) particles were coated with liposomal membrane contained hematoporphyrin with neoplastic affinity.In Vivo Studies of Rabbits: We used high frequency inductive heater (NIHON KOSHUHA CO. LTD. YKN-10), frequency: 4OOKHz, out put: 1OKw, coil diameter: 90mm. VX-2 tumor cells at 1.5X10^7 were implanted to the lower thigh of rabbit. When the implanted tumor grew up 3cm in diameter, A-D group were heated and studied.Group A: In a dose of 200mg/kg of HP-LM was administrated into the femoral artery, Group B: In a dose of 100mg/kg of Fe_3 O_4 was administrated into the femoral artery, Group C … More : Ligation of femoral artery, Group D: Nonadministrated, and then was immediately heated.Group A: In a dose of 200mg/kg of HP-LM, with heating for 10 minutes, temperature was elevated 14.8ﾟC at center of tumor. Group B: In a dose of 100mg/kg of Fe_3 O_4, with heating for 10 minutes, temperature was elevated 10.6ﾟC at center of tumor. Group C: In a ligation of femoral artery, with heating for 10 minutes, temperature was elevated 6.5ﾟC at center of tumor. Group D: In nonadministration, with heating for 10 minutes, temperature was elevated 4.4ﾟC at center of tumor. Temperature elevation of Group A was significantly higher in comparison with those found in another group. In histological features in Group A, iron particles were stained in blood capillaries at center and edge of tumors, and in tumor cells.It was thought that this technique is very useful treatment, because selective hyperthermia could be obtained by high frequency inductive heating of HP-LM, as a result of adsorption of HP-LM to tumor tissue. Less

在将铁磁性颗粒注入肿瘤后，可以通过应用高频感应加热来更有选择性地加热肿瘤。为了设计一种用于肿瘤高频感应加热的选择性热疗技术，我们继续使用脂质体铁磁性微粒(HP-LM)进行基础研究，制备方法如下：将四氧化三铁(Fe_3O_4)微粒包裹在含有具有肿瘤亲和力的血卟啉的脂质体上。在兔体内研究中：我们使用高频感应加热器(Nihon KOSHUHA)。LTD.YKN-10)，频率：400 KHz，输出：10KW，线圈直径：90 mm。将1.5×10~(-7)的VX-2肿瘤细胞接种于兔大腿下段。A组：股动脉注射HP-LM 200 mg/kg，B组：股动脉注射Fe3O_4 100 mg/kg，C组…。A组：HP-LM 200 mg/kg，加热10min，肿瘤中心温度升高14.8ﾟC。B组：Fe_3O_4 100 mg/kg，加热10min，肿瘤中心温度升高10.6ﾟC。C组：结扎股动脉，加热10min，肿瘤中心温度升高6.5ﾟC。D组：非给药组，加热10min，肿瘤中心温度升高4.4ﾟC。A组的体温升高明显高于另一组。在组织学特征方面，A组肿瘤中心和边缘的毛细血管内及肿瘤细胞内均可见铁颗粒染色，由于HP-LM吸附在肿瘤组织上，可通过高频感应加热HP-LM获得选择性热疗，故认为该技术是一种非常有用的治疗方法。较少

项目成果

SATOSHI SUZUKI: "STUDIES ON LIPOSOMAL FERROMAGNETIC PARTICLES AND A TECHNIQUE OF HIGH FREQUENCY INDUCTIVE HEATING(REPORT II)" HYPERTHERMIC ONCOLOGY 1988. 1. 236-238 (1989)

铃木聪：“脂质体铁磁颗粒和高频感应加热技术的研究（报告II）”热肿瘤学1988.1.236-238（1989）

SATOSHI SUZUKI: "STUDIES ON LIPOSOMAL FERROMAGNETIC PARTICLES AND A TECHNIQUE OF HIGH FREQUENCY INDUCTIVE HEATING (REPORT II)" HYPERTHERMIC ONCOLOGY 1988, 1, 236-238, 1989.

Satoshi SUZUKI：“脂质体铁磁颗粒和高频感应加热技术的研究（报告 II）”《热疗肿瘤学》1988 年，1，236-238，1989。