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Assessment of graft viability after cold preservation in small intestinal transplantation.

小肠移植冷保存后移植物活力的评估。

基本信息

批准号：
04670785
负责人：
TAGUCHI Tomoaki
金额：
$ 1.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The precise assessment of graft viability is thought to be important, however there are still a few methods which remain controversial. We investigated the method for the assessment of small bowel graft viability, by means of physiological properties, energy metabolism, tissue blood flow, and the histological findings.Syngeneic heterotopic transplantation was performed using Lewisrats, after a cold preservation of a jejunal graft with UW solution for 6, 12, 24, or 48hr. Graft samples were taken before transplantation for physiological study, and at harvesting, at the end of cold storage, before and 30 min after reperfusion for histology and for the measurement of adenine nucleotides (HPLC method). The tissue blood flow of the graft was recorded using a laserdoppler flowmeter at reperfusion.The positive rates of samples showing both the biphasic nervous activities (excitation and inhibition) and the spontaneous rhythmic activity was 100%, 88%, and 25% in 0, 24, and 48 hr preservation, respectively. The level of ATP after reperfusion, which recovered to more than 30% of that at harvesting within 24 hr preservation groups but remained at less than 30% in the 48 hr group. The blood flow level at 5 or 30 min of reperfusion was significantly lower in the 48 hr group than that in the other groups. Histological damage after reperfusion, which extended deep into the crypt layr in the 48 hr group, but was confinded within villi in the other groups, correlated well with the results of physiology, energy metabolism, and tissu blood folw.The physiological property before transplantation, and the tissue ATP level and blood flow after reperfusion are considered to be useful parameters for assessment of small bowel graft viability. These results are correlated with the recipient survival which we had previously reported.

移植物活性的精确评估被认为是重要的，但是仍然有一些方法仍然存在争议。本研究通过生理学特性、能量代谢、组织血流量和组织学检查，探讨了评价小肠移植物存活的方法。采用Lewis大鼠进行同系异位移植，空肠移植物用UW液冷保存6、12、24或48小时。在移植前采集移植物样品用于生理学研究，在收获时、冷藏结束时、再灌注前和再灌注后30 min采集移植物样品用于组织学和用于腺嘌呤核苷酸的测量（HPLC法）。再灌注时用激光多普勒血流仪记录移植物组织血流量，保存0、24和48小时时，双相神经活动（兴奋和抑制）和自发节律活动的标本阳性率分别为100%、88%和25%。再灌注后ATP水平在24小时内恢复到收获时的30%以上，但在48小时组仍低于30%。再灌注5min和30min时，48h组的血流量明显低于其他各组。再灌注48小时组小肠组织损伤深入到隐窝层，其余各组小肠组织损伤局限于绒毛内，与生理、能量代谢和组织血流量结果有良好的相关性，移植前的生理特性、再灌注后组织ATP水平和组织血流量可作为评价移植小肠存活的有用指标。这些结果与我们先前报道的受体存活率相关。