Characterization of bloody cerebrospinal fluid after SAH.

SAH 后血性脑脊液的特征。

• 批准号: 04670856
• 负责人: YAMADA Hiromu
• 金额: $ 1.09万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670856/
• 关键词: Vasospasm; calcium; smooth muscle cell; nitric oxide; hemoglobin; cerebrospinal fluid; くも膜下出血; 脳血管攣縮; 培養平滑筋細胞; 細胞骨格蛋白質; Subarachnoid hemorrhage; Uascular smooth muscle cell; Cerebrospinal fluid; Phospholipase C; Intracellular calcium; Plate

To understand the mechanism of cerebral vasospasm after subarachnoid hemorrhage, we studied intra- or sub-cellular responses (e. q. intracellular calcium concentration, contractile related proteins, arachidonic acid, nitric oxide, and nitric oxide synthase) in cultured smooth muscle cells and endothelial cells after stimultion of oxyhemoglobin or hemolysate, which is thought to be candidate for cerebral vasospasm. Unknown substance in cerebrospinal fluid after subarachnoid hemorrhage, which has the ability of induction of intracellular calcium elevation in smooth muscle cells, was found. In this paper, these results using cultured cells were reviewed.

为了了解蛛网膜下腔出血后脑血管痉挛的机制，我们研究了培养的血管平滑肌细胞和内皮细胞在氧合血红蛋白或溶血物刺激后的细胞内或亚细胞反应(如细胞内钙离子浓度、收缩相关蛋白、花生四烯酸、一氧化氮和一氧化氮合酶)，这被认为是脑血管痉挛的候选因素。蛛网膜下腔出血后脑脊液中发现具有诱导细胞内钙离子升高能力的未知物质。本文综述了利用培养细胞获得的这些结果。

项目成果

Katsunobu Takenaka, Junji Kishino, Hiromu Yamada, Noboru Sakai, Nitoshi Arita, Yukio Okano, Yoshinori Nozawa: "DNA synthesis and intracellular calcium elevation in porcine cerebral arterial smooth muscle cells by cerebrospinal fluid from patients with sub

Katsunobu Takenaka、Junji Kishino、Hiromu Yamada、Noboru Sakai、Nitoshi Arita、Yukio Okano、Yoshinori Nozawa：“亚健康患者脑脊液对猪脑动脉平滑肌细胞的 DNA 合成和细胞内钙升高的影响

Katsunobu Takenaka, Junji Koshino, Hitoshi Arita, Yukio Okano, Noboru Sakai, Hiromu Yamada, Yoshinori Nozawa: "Biochemical activity of the endothelin family in cultured basilar arterial smooth muscle cells" Neuroligical Research. 15. 29-32 (1993)

Katsunobu Takenaka、Junji Koshino、Hitoshi Arita、Yukio Okano、Noboru Sakai、Hiromu Yamada、Yoshinori Nozawa：“培养的基底动脉平滑肌细胞中内皮素家族的生化活性”神经学研究。

Toshihiko Nakashima, Katsunobu Takenaka, Yasuaki Nishimura, Takashi Andoh, Noboru Sakai, Hiromu Yamada, Yoshiko Banno, Yukio Okano, Yoshinori Nozawa: "Phospholipase C activity in cerebrospinal fluid following subarachnoid hemorrhage related to brain damag

Toshihiko Nakashima、Katsunobu Takenaka、Yasuaki Nishimura、Takashi Andoh、Noboru Sakai、Hiromu Yamada、Yoshiko Banno、Yukio Okano、Yoshinori Nozawa：“与脑损伤相关的蛛网膜下腔出血后脑脊液中的磷脂酶 C 活性

T Iwai et al: "Hypothermic prevention of the hippocampal damage following ischemia in morgollian gerbils comparison between intraischemic and brief postischemic hypothermia" Life Sciences. 52. 1031-1038 (1993)

T Iwai 等人：“低体温预防沙鼠缺血后海马损伤的缺血内和短暂缺血后低温的比较”生命科学。

Kastunobu Takenaka: "Biochemical activity of the endothelin family in cultured basilar arterial smooth muscle cells" Newrological Research. 15. 29-32 (1993)

Kastunobu Takenaka：“培养的基底动脉平滑肌细胞中内皮素家族的生化活性”Newrological Research。