Study on mechanisms of intravenous anesthetic agents by ^1H-NMR

我们通过测量生物聚合物到水的自旋晶格弛豫时间（T_1）和分子间交叉弛豫时间（T_<IS>），研究了麻醉药组织中的水结构，从而明确了静脉麻醉药的作用机制。这是非常重要的，在生物标本上进行的^1H-NMR实验的主要部分是使用内径1.1mm的玻璃毛细管，将其插入含有少量溶剂的5mmphi核磁共振管中进行锁定，并使用带有中心孔的小棒状塑料间隔片。方法(A)、(B)、(C)与藤田卫生大学综合医学研究所分子生理学系合作，采用大鼠肝脏或脑组织进行基础研究。方法(A)：将内径为1.1mm、两端打开的核磁共振玻璃毛细血管在转入大鼠肝脏或大脑时刺穿，待组织进入其中一端后，用塑料浆糊将毛细血管两端封闭。更多的毛细血管，用瞬时黏合剂覆盖组织侧浆糊后，将填充D_2O的5mmphi核磁共振管的大部分插入中心孔中进行锁定。方法(B)：将方法(A)整理好，用75ml的微管刺穿组织后，用一端内径1.1mm的封闭玻璃毛细血管进行穿刺，用塑料膏封住开口一侧，按方法(A)插入填充二甲亚砜-d_6的5mmphi核磁共振管中进行锁定。方法(C)：按照方法(B)将内径1.1mm的NMR玻璃毛细血管插入专用的NMR试管中；内径3.2mm，外径3.98mm，将其插入5mmphi的核磁共振管中，并加入少量二甲亚砜-d_6。对采用(A)、(B)、(C)方法制备的生物标本，用Brucker AM500核磁共振谱仪研究了T_1和t__ <IS>的控制和收割难易程度。结果表明，调光控制非常容易，结果表明，我们可以测量T_1和t__ <IS>的方法(C)。少

We studied on water structure in the tissues for anesthetic agents by measuring spin-lattice relaxation times (T_1) and intermolecular cross-relaxation times (T_<IS>) from biopolymer to water, as a result of making clear for mechanisms of intravenous anesthetic agents. This is very important that the major parts of ^1H-NMR experiments on biospecimens are carried out using glass capillaries of 1.1mm inner diameter, inserted into a 5mmphi NMR tube containing a small volume of solvent for locking, with small rod-shaped plastic spacers with a center hole. We conceived the methods (A), (B) and (C), included basic studies using liver or cerebral tissues of rats with department of molecular physiology, comprehensive medical institute of Fujita Health University. Method (A) : NMR glass capillaries of 1.1mm inner diameter with both ends open were pierced as turning to a rat liver or cerebrum, closed up the both ends of the capillaries by plastic paste after the tissues got into one end of the c … More apillaries, and inserted into the center hole for the most part of a 5mmphi NMR tube filled up D_2O for locking after covered the tissue side paste by an instantaneous adhesive agent. Method (B) : Arranged the method (A), carried out using one end closed glass capillaries of 1.1mm inner diameter after pierced a 75mul micropipette to the tissue, closed up the open side by plastic paste, and inserted to a 5mmphi NMR tube filled up dimethylsulfoxide-d_6 for locking according to the method (A). Method (C) : After inserted NMR glass capillaries of 1.1mm inner diameter according to method (B) to a special test tube for NMR ; 3.2mm inner & 3.98mm outside diameter, that was inserted to a 5mmphi NMR tube with a small quantity of dimethylsulfoxide-d_6. For the biospecimens that made by the methods (A), (B) and (C), we studied degree of difficulties to shimming control and reappering of T_1 and T_<IS> by Brucker AM500 NMR spectrometer. In results, shimming control was very readily, and turned out as we could measuring of T_1 and T_<IS> for the mothod (C). Less