Study on mechanisms of intravenous anesthetic agents by ^1H-NMR

^1H-NMR研究静脉麻醉药作用机制

• 批准号: 04670943
• 负责人: KURODA Toshihisa
• 金额: $ 1.15万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670943/
• 关键词: intravenous anesthetic agents; water structure; ^1H-NMR; spin lattice relaxation times (T_1); intermolecular cross-relaxation times (T_<IS>); 静脈麻酔薬作用機序; 分子間交差緩和時間(T_<1S>); 静脈麻酔薬; 分子交差緩和時間(T_<IS>)

We studied on water structure in the tissues for anesthetic agents by measuring spin-lattice relaxation times (T_1) and intermolecular cross-relaxation times (T_<IS>) from biopolymer to water, as a result of making clear for mechanisms of intravenous anesthetic agents. This is very important that the major parts of ^1H-NMR experiments on biospecimens are carried out using glass capillaries of 1.1mm inner diameter, inserted into a 5mmphi NMR tube containing a small volume of solvent for locking, with small rod-shaped plastic spacers with a center hole. We conceived the methods (A), (B) and (C), included basic studies using liver or cerebral tissues of rats with department of molecular physiology, comprehensive medical institute of Fujita Health University. Method (A) : NMR glass capillaries of 1.1mm inner diameter with both ends open were pierced as turning to a rat liver or cerebrum, closed up the both ends of the capillaries by plastic paste after the tissues got into one end of the c … More apillaries, and inserted into the center hole for the most part of a 5mmphi NMR tube filled up D_2O for locking after covered the tissue side paste by an instantaneous adhesive agent. Method (B) : Arranged the method (A), carried out using one end closed glass capillaries of 1.1mm inner diameter after pierced a 75mul micropipette to the tissue, closed up the open side by plastic paste, and inserted to a 5mmphi NMR tube filled up dimethylsulfoxide-d_6 for locking according to the method (A). Method (C) : After inserted NMR glass capillaries of 1.1mm inner diameter according to method (B) to a special test tube for NMR ; 3.2mm inner & 3.98mm outside diameter, that was inserted to a 5mmphi NMR tube with a small quantity of dimethylsulfoxide-d_6. For the biospecimens that made by the methods (A), (B) and (C), we studied degree of difficulties to shimming control and reappering of T_1 and T_<IS> by Brucker AM500 NMR spectrometer. In results, shimming control was very readily, and turned out as we could measuring of T_1 and T_<IS> for the mothod (C). Less

我们通过测量生物聚合物到水的自旋-晶格驰豫时间(T_1)和分子间交叉驰豫时间(T_1)来研究麻醉剂组织中的水结构，以阐明静脉麻醉剂的作用机理。这一点非常重要，因为生物样品的~1H-核磁共振实验的主要部分是使用内径1.1 mm的玻璃毛细管，插入含有用于锁定的小体积溶剂的5Mmphi核磁共振管中，带有中心孔的小棒状塑料隔板。我们设想的方法(A)、(B)和(C)，包括利用藤田保健大学综合医学部分子生理学教研室的大鼠肝脏或脑组织进行的基础研究。方法(A)：将内径1.1 mm、两端开放的核磁共振玻璃毛细血管穿入大鼠肝脏或大脑，组织进入c-…一端后，用塑料糊剂封闭毛细血管两端。用瞬时粘结剂覆盖组织侧糊剂后，将更多的毛细血管插入5 mm×10 mm核磁共振管的中心孔内，填充D_2O进行锁定。方法(B)：安排方法(A)，用一端内径1.1 mm的闭合玻璃毛细管将75 mm的微管穿入组织，用塑料糊剂封闭开放侧，按方法(A)插入装满二甲基亚砜-d_6的5 mm核磁共振管锁定。方法(C)：按方法(B)将内径1.1 mm的核磁共振玻璃毛细管插入内径为3.2 mm、外径为3.98 mm的核磁共振专用试管中，插入含有少量二甲基亚砜-d_6的5 mm核磁共振管中。对于采用方法(A)、(B)和(C)制备的生物样品，用Brucker AM500型核磁共振仪研究了T_1和T_1、T_1和T_1的垫片控制和再生困难程度。在结果中，垫片控制是非常容易的，结果是我们可以测量方法(C)的T_1和T_1。较少