Effects of Growth and thyroid hormones on the renal development in hormone-deficiency mice.

为探讨生长激素（GH）和甲状腺激素（T_4）对肾脏发育的影响，我们观察了出生后20天内单独或联合给予GH和T_4对Snell垂体侏儒小鼠（dw）肾脏发育的影响。虽然未处理的dw小鼠表现出体重和肾脏重量减少，皮质和髓质的深度小，肾小球直径减小，单位面积上肾小球数目增加，但这些异常在出生后的第二个20天内通过激素处理得到了有利的改善。组织学上，观察到dw肾脏中的小肾小管细胞和小细胞组织化肾小球通过治疗改善至对照水平。在这个实验中，它被揭示了T4是有效的生长激素对肾脏发育。在先天性原发性甲状腺功能减退小鼠（hyt）肾脏中，也存在dw小鼠中发现的异常，但程度较轻。为了阐明生长激素（GH）和甲状腺素（T_4）对肾发育的影响，我们对不同发育阶段的dw小鼠肾组织进行了生化和形态学观察。侏儒小鼠和正常对照小鼠的DNA、RNA和胰岛素样生长因子1（IGF-1）含量的差异在10日龄时开始明显，此后侏儒小鼠的DNA和RNA含量不再增加，IGF-1含量下降，而正常对照小鼠的DNA和RNA含量继续增加。提示GH和T_4对肾脏发育可能有影响。此外，从10日龄开始，这些激素的缺乏明显影响肾脏发育。

In order to elucidate the effects of growth(GH) and thyroid(T4) hormones on renal development, We examined the kidneys of Snell pituitary dwarf mouse(dw) after the administration of these hormones, either single or in conbination, during the second 20days of postnatal life. Although the untreated dw mice showed reduced body and kidney weights, small depths of cortex and medulla, reduced diameter of glomeruli, and increased nimber of glomeruli on a unit area basis, these abnormalities were favorably improved by the hormonal treatment during the second 20 days of postnatal life. Histologically, the small renal tubular cells and small cells organized glomeruli obseved in the dw kidney were ameliorated to the levels of controls by the treatment. In this experiment, it was revealed that T4 is as effective as GH on renal development. In the case of the congenital primary hypothyroid mouse(hyt) kidney, the abnormalities found in the dw mouse were also present, but to a lesser extent. Therefore, these results sugest that GH is indispensable for renaldevelopment and the effect of T4 is considered to be complementary or synergistic.In order to elucidate the effects of growth hormone(GH) and thyroxine(T4) on renal development we biochemically and morphologically examined the renal tissue of dw mouse at different development. Differences in DNA RNA and insurin-like growth factor 1(IGF-1) content between the dwarf and normal control mice first became apparent on the 10th dat of age, and from this time that of dwarf mice showed no further increase in DNA and RNAcontent and decrease in IGF-1 content whereas that of the normal control mice continued to increase. These results indicated that GH and T4 may have effects on the renal development. Furthermore, it was apparent that deficienty of these hormone became to affect renal development from 10 day of age.