权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effects of Growth and thyroid hormones on the renal development in hormone-deficiency mice.

生长激素和甲状腺激素对激素缺乏小鼠肾脏发育的影响。

基本信息

批准号：
04670983
负责人：
MATSUSHIMA Masahiro
金额：
$ 0.45万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670983/
关键词：
Snell dwarf mouse renal development congenitally hypothyroid mouse kidney growth hormone thyroid hormone DNA IGF-1

项目摘要

In order to elucidate the effects of growth(GH) and thyroid(T4) hormones on renal development, We examined the kidneys of Snell pituitary dwarf mouse(dw) after the administration of these hormones, either single or in conbination, during the second 20days of postnatal life. Although the untreated dw mice showed reduced body and kidney weights, small depths of cortex and medulla, reduced diameter of glomeruli, and increased nimber of glomeruli on a unit area basis, these abnormalities were favorably improved by the hormonal treatment during the second 20 days of postnatal life. Histologically, the small renal tubular cells and small cells organized glomeruli obseved in the dw kidney were ameliorated to the levels of controls by the treatment. In this experiment, it was revealed that T4 is as effective as GH on renal development. In the case of the congenital primary hypothyroid mouse(hyt) kidney, the abnormalities found in the dw mouse were also present, but to a lesser extent. Therefore, these results sugest that GH is indispensable for renaldevelopment and the effect of T4 is considered to be complementary or synergistic.In order to elucidate the effects of growth hormone(GH) and thyroxine(T4) on renal development we biochemically and morphologically examined the renal tissue of dw mouse at different development. Differences in DNA RNA and insurin-like growth factor 1(IGF-1) content between the dwarf and normal control mice first became apparent on the 10th dat of age, and from this time that of dwarf mice showed no further increase in DNA and RNAcontent and decrease in IGF-1 content whereas that of the normal control mice continued to increase. These results indicated that GH and T4 may have effects on the renal development. Furthermore, it was apparent that deficienty of these hormone became to affect renal development from 10 day of age.

为了阐明生长激素(GH)和甲状腺激素(T4)对肾脏发育的影响，我们对出生后第二个20天的Snell垂体性侏儒鼠(DW)单独或联合使用这两种激素后的肾脏进行了观察。虽然未经治疗的dw小鼠体重和肾脏重量减轻，皮质和髓质深度变小，肾小球直径减小，单位面积肾小球数目增加，但在出生后第二个20天内进行激素治疗后，这些异常情况得到了有利的改善。组织学上，肾小管细胞和小细胞有组织的肾小球通过治疗改善到对照组的水平。在这项实验中，发现T4在肾脏发育方面与GH一样有效。在先天性原发甲状腺功能减退小鼠(Hyt)的肾脏中，dw小鼠也存在异常，但程度较小。为了阐明生长激素(GH)和甲状腺素(T4)对肾脏发育的影响，我们对不同发育阶段的DW小鼠肾脏组织进行了生化和形态观察。矮小鼠DNA、RNA和IGF-1含量的差异在10日龄时开始明显，此后矮小鼠的DNA和RNA含量不再增加，IGF-1含量下降，而正常对照组的含量继续增加。这些结果表明，GH和T4可能对肾脏发育有影响。此外，很明显，这些激素的缺乏从10日龄就开始影响肾脏的发育。