Study on Testicular in Animals with Congenital Hormone Deficiency

先天性激素缺乏动物睾丸的研究

• 批准号: 02670724
• 负责人: MATSUSHIMA Masahiro
• 金额: $ 1.22万
• 依托单位: Toho University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670724/
• 关键词: spermatogenesis; Snell dwarf mouse; congenitally hypothyroid mouse; little mouse; testis; kidney; DNA; RNA

The status of spermatogenesis comparing, , with kidney and the biochemical study on testicular tissue in Snell dwarf, little and congenitally hypothyroid mice was studied.1. In all of these mice with a hormondeficiency the testicular weight were smaller, the seminiferous tubules were smaller in size and the total number. of sperm-generating cells, which consist of spermatogonia, spermatocytes, spermatids and sperms, showed remarkable decreases, compared with each control group, and the decrease in total cell number was improved favorably by treatment with hormone. In comparison with the control groups, significant decreases were showed in the spermatid number for the dw/dw group, sperm number for the lit/lit group, and spermatid and sperm numbers for the hyt/hyt group.2. On the other hand, the weight of kidney, the depth of renal cortex and medulla showed remarkable decreases, compared with each control group, and the decrease of the weight of kidney and the depth of renal cortex and medulla were improved favorably by treatment with hormone. But the number of glomeruli in mm^2 showed remarkable increases, compared with each control group, and the increases of them were improved by such replacement therapy.These results indicate that GH and T_4 have a potential effect on sperm-generating function and renal structure.3. In comparison with the control groups, significant decreases were showed in total DNA content, total RNA content and RNA/DNA ratio for the dw/dw group and the hyt/hyt group. But the protein, DNA and RNA contents in G-wet-weight were not significant different for these groups. The poliamine contents (total of putrecine, spermine and cadavarine content) showed significant increases for the dw/dw group and hyt/hyt group.

本文研究了Snell矮型、小型和先天性甲状腺功能减退小鼠与肾脏的精子发生情况及睾丸组织的生化研究。在所有这些激素缺乏的小鼠中睾丸重量更小，精小管的大小和总数也更小。由精原细胞、精母细胞、精母细胞和精子组成的生精细胞数量与各对照组相比均显著减少，且激素处理能较好地改善生精细胞总数的减少。与对照组相比，dw/dw组精子数量显著减少，lit/lit组精子数量显著减少，hyt/hyt组精子数量和精子数量显著减少。另一方面，与各对照组相比，肾脏重量、肾皮质和肾髓质深度均显著降低，且激素治疗对肾脏重量和肾皮质和肾髓质深度的降低有较好的改善作用。但与各对照组相比，mm^2肾小球数量明显增加，且经替代治疗后，肾小球数量的增加有所改善。这些结果表明GH和T_4对精子生成功能和肾脏结构有潜在的影响。与对照组相比，dw/dw组和hyt/hyt组的总DNA含量、总RNA含量和RNA/DNA比值均显著降低。g -湿重中蛋白质、DNA和RNA含量各组间差异不显著。dw/dw组和hyt/hyt组的poliamine含量（腐胺、精胺和尸胺总含量）显著升高。

项目成果

原田 昌幸: "先天性内分泌異常マウスにおける腎成長に及ぼすホルモン効果の検討" 東邦医学会誌. 38. (1992)

Masayuki Harada：“检查激素对先天性内分泌异常小鼠肾脏生长的影响”东邦医学会杂志 38。（1992）

原田 昌幸,黒田 加奈美,杉崎 哲朗,松島 正浩: "先天性ホルモン欠損動物の泌尿生殖器系臓器の発育に及ぼすホルモン効果について" 東邦医学会誌. 38. 780-788 (1992)

Masayuki Harada、Kanami Kuroda、Tetsuro Sugisaki、Masahiro Matsushima：“激素对先天性激素缺乏动物泌尿生殖器官发育的影响”东邦医学会杂志 38. 780-788 (1992)。

原田 昌幸,柳下 次雄,杉崎 哲朗,松島 正浩: "先天性ホルモン欠損マウスの精巣の生化学的研究" 東邦医学会誌. 38. (1992)

Masayuki Harada、Ttsuguo Yanagishita、Tetsuro Sugisaki、Masahiro Matsushima：“先天性激素缺乏小鼠睾丸的生化研究”东邦医学会杂志 38。（1992）。

Masayuki Harada, Tsuguo Yagishita, Tetsurou Sugizaki and Masahiro Matsushima: "Biochemical study on testicular tissue in animals with congenital hormonedeficiency." J. Med. Soc. Toho. 39. (1992)

Masayuki Harada、Ttsuguo Yagishita、Tetsurou Sugizaki 和 Masahiro Matsushima：“先天性激素缺乏症动物睾丸组织的生化研究。”

Masayuki Haraka, Kanami Kuroda, Tetsurou Sugizaki and Masahiro Matsushima: "Study on testicular and renal tissue in animal with congenital hormone deficiency" J. Med. Soc. Toho. 38. 780-788 (1992)

Masayuki Haraka、Kanami Kuroda、Tetsurou Sugizaki 和 Masahiro Matsushima：“先天性激素缺乏动物睾丸和肾组织的研究”J. Med。