The effect of diabetes mellitus on periodontitis

糖尿病对牙周炎的影响

• 批准号: 04671147
• 负责人: OHURA Kiyoshi
• 金额: $ 1.34万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671147/
• 关键词: diabetes mellitus; gingivitis; leukocyte; rat; chemotaxis; phagocytosis; bactericidal activity; insulin; 歯周炎; マクロファージ; 貧食能

Streptozotocin (STZ) was intravenously administrated to rats with naturally occurring gingivitis (Sus) and normal rats to make diabetic animals for the experiment. To study the effect of blood glucose (BG) control, diabetic rats were divided into two groups 4 weeks after STZ injection, and insulin was subcutaneously administrated once a day to rats of one group. Pocket probing depth (PD), BG, HbA1C, superoxide dismutase (SOD) level, and serum insulin level of each rat were measured. To elucidate the effects of diabetes on periodontitis, leukocyte functions such as chemotaxis, phagocytosis and bactericidal activity of both rat peritoneal polymorphonuclear leukocytes (PMNLs) and macrophages (Mphis) were measured. Leukocyte functions of diabetic Sus (Sus-STZ) were lower than those of normal, Sus, and insulin treated Sus-STZ.Also PDs of Sus-STZ were significantly deeper than those of Sus (p<0.05), and this indicates that diabetes makes periodontitis much worse. Both phagocytosis ratio (PR) and phagocytosis index (PI) of Mphis from Sus-STZ were significantly lower than those of any other rats (p<0.05). Both PR and PI of Mphis from Sus-STZ and Sus showed negative correlations with BG.Similar results were obtained with Mphi chemotaxis and bactericidal activity. These results indicate that suppression of leukocyte functions affects host defense mechanisms and therefore periodontitis acompanied by diabetes becomes worse or resists to treatment. We are planning to study the effect of insulin treatment of both short and long term upon the severity of periodontitis and leukocyte functions.

将链脲佐菌素（STZ）静脉注射给患有自然牙龈炎（Sus）的大鼠和正常大鼠，制成糖尿病动物进行实验。为了研究血糖（BG）控制效果，将糖尿病大鼠在注射STZ后4周分为两组，其中一组大鼠皮下注射胰岛素，每天一次。测量每只大鼠的口袋探诊深度（PD）、BG、HbA1C、超氧化物歧化酶（SOD）水平和血清胰岛素水平。为了阐明糖尿病对牙周炎的影响，测量了大鼠腹膜多形核白细胞（PMNL）和巨噬细胞（Mphis）的白细胞功能，例如趋化性、吞噬作用和杀菌活性。糖尿病Sus (Sus-STZ)的白细胞功能低于正常Sus、Sus和胰岛素治疗Sus-STZ的白细胞功能。而且Sus-STZ的PD明显比Sus更深(p<0.05)，这表明糖尿病使牙周炎更加严重。 Sus-STZ中Mphis的吞噬率（PR）和吞噬指数（PI）均显着低于任何其他大鼠（p<0.05）。 Sus-STZ和Sus的Mphis的PR和PI均与BG呈负相关。Mphi的趋化性和杀菌活性也得到了类似的结果。这些结果表明，白细胞功能的抑制会影响宿主的防御机制，因此伴有糖尿病的牙周炎会恶化或难以治疗。我们计划研究短期和长期胰岛素治疗对牙周炎严重程度和白细胞功能的影响。

项目成果

K.Ohura: "Oxygen Radicals" Elsevier Science Publishers. 69-72 (1992)

K.Ohura：“氧自由基”爱思唯尔科学出版社。

Keigo Ogata: "Effect of streptozotocin-induced diabetes on macrophage phagocytosis in rats with naturally occurring gingivitis." The Japanese Journal of Pharmacology. 61. 162- (1993)

Keigo Ogata：“链脲佐菌素诱导的糖尿病对自然发生牙龈炎的大鼠巨噬细胞吞噬作用的影响。”

Mitsuko Shinohara: "Influence of streptozotocin diabetes on periodontal disease." The Japanese Journal of Pharmacology. 61. 335- (1993)

Mitsuko Shinohara：“链脲佐菌素糖尿病对牙周病的影响。”

尾形 圭五: "歯肉炎自然発症ラットのマクロファージ貪食能に及ぼす糖尿病の影響について" 歯科基礎医学会雑誌. 35. 209 (1993)

Keigo Ogata：“糖尿病对自发性牙龈炎大鼠巨噬细胞吞噬作用的影响”日本基础牙科医学会杂志 35. 209 (1993)。

K.Ogata: "Effect of streptozotocin induced diabetes on macrophage phagocytosis in rats with naturally occurring gingivitis." Jap.Oral Biology. 35. 209 (1993)

K.Ogata：“链脲佐菌素诱导的糖尿病对患有自然牙龈炎的大鼠巨噬细胞吞噬作用的影响。”