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Studies on leukocyte function of periodontal desease and diabetes mellitus

牙周病和糖尿病白细胞功能的研究

基本信息

批准号：
06671896
负责人：
OHURA Kiyoshi
金额：
$ 1.22万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671896/
关键词：
diabetes mellitus gingivitis periodontal disease rat leukocyte macrophage chemotaxis phagocytosis 白血球貧食能歯周炎

项目摘要

Many studies have shown that periodontal lesions in diabetic patients progress rapidly and resist treatment. Herein, we use the naturally occurring gingivitis rat (ODUS/Odu) with diabetes mellitus induced by injection of streptozotocin (STZ : 65mg/kg, i.v.) and the control rat (ODUS/Odu without STZ). One and 3 months after STZ injection, we intraperitoneally injected liquid paraffin. Four days later, we measured the pocket probing depth (PPD), and collected the blood sample and peritoneal macrophages from these rats. To elucidate the effect of diabetes on periodontal lesions, the chemotaxis and phagocytosis of macrophages were studied in ODUS/Odu with and without diabetes. One and 3 months after STZ injection, the PPD in the ODUS/Odu with diabetes was significantly deeper than in ODUS/Odu without diabetes (p<0.001). PPD was deeper at 3 months after STZ injection than at 1 month in ODUS/Odu with diabetes. Three months after STZ injection, there was a high positive correlation between blood glucose and triglycerides, or hemoglobin A_1C (p<0.01). In both experimental periods, the phagocytosis of macrophages in the diabetes group was significantly more suppressed than in that without diabetes (p<0.001). Three months after STZ injection, the macrophage chemotaxis was more suppressed in ODUS/Odu with diabetes than in those without diabetes. These findings stuggest that diabetes mellitus affects the chemotaxis and phagocytosis of macrophages in ODUS/Odu. Therefore, the host defense mechanism weakens, and worsens periodontal disease.

许多研究表明，糖尿病患者的牙周病变进展迅速，并且对治疗产生抵抗力。在此，我们使用注射链脲佐菌素（STZ：65mg/kg，静脉注射）诱导的患有糖尿病的天然牙龈炎大鼠（ODUS/Odu）和对照大鼠（不含STZ的ODUS/Odu）。注射STZ后1个月和3个月，我们腹腔注射液体石蜡。四天后，我们测量了口袋探查深度（PPD），并收集了这些大鼠的血样和腹膜巨噬细胞。为了阐明糖尿病对牙周病变的影响，我们研究了患有和不患有糖尿病的 ODUS/Odu 中巨噬细胞的趋化性和吞噬作用。注射 STZ 后 1 个月和 3 个月，患有糖尿病的 ODUS/Odu 中的 PPD 显着深于未患糖尿病的 ODUS/Odu 中 (p<0.001)。患有糖尿病的 ODUS/Odu 患者注射 STZ 后 3 个月时的 PPD 比 1 个月时更深。注射STZ后三个月，血糖与甘油三酯或血红蛋白A_1C之间存在高度正相关（p<0.01）。在两个实验期间，糖尿病组巨噬细胞的吞噬作用均比非糖尿病组明显受到抑制（p<0.001）。注射 STZ 三个月后，患有糖尿病的 ODUS/Odu 中的巨噬细胞趋化性比非糖尿病患者受到更多抑制。这些发现表明糖尿病影响 ODUS/Odu 中巨噬细胞的趋化性和吞噬作用。因此，宿主防御机制减弱，牙周病恶化。