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Sutudy of vascular reactions in response to hemodynamic factors assessed by stretch activation

通过拉伸激活评估血流动力学因素的血管反应研究

基本信息

批准号：
04671360
负责人：
NAKAYAMA Koichi
金额：
$ 1.34万
依托单位：
Uuniversity of Shizuoka
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Cerebral and coronary arteries are extremely sensitive to hemodynamic factors, i.e., biomichacal stimule, including blood pressure and blood flow. I have investigated the ca^<2+>-signaling mechanisms of mechanical reception in the cardiovascular system from the following point of view, and as a leader i have promoted the progress of this relatively underdeveloped research field. I have regarded the myogenic contractile reaction of blood vessels in response to stretch of vascular wall or an increase in intraluminal pressure as a kind of cellular/intracellular and Ca^<2+> signaling ; and 3) the cross-bridge property of contractile elements. I could have showed interesting results as follows which will promise the future progress in the research. I have found that membrane fluidity strongly affects the sensitivity of a mechano-receptor site, and an amiloride-sensitive component, a possible stretch-activated channel, may play a role in the genesis of myogenic activity. In addition, the biomechanical stimulus augmented the activity of phospholipase C, production of inositol trisphosphate, and increase in cytosolic Ca^<2+> concentration, which indicates a possible role of GTP-binging proteins coupled with this enxyme in the transformation of mechanical stimulus to cellular signalings. Moreover, myogenic activity is found to be in-between the contraction produced by phorbol esters and high K^+ depolarizing stimulus in the property of cross-bridge cycling. These results suggest that vascular reaction in response to biomechanical stimuli is mediated through a novel mechanisum of cellular signaling.

大脑和冠状动脉对血液动力学因素非常敏感，即生物刺激，包括血压和血流量。我从以下角度研究了心血管系统机械接收的钙信号机制，并作为领导者推动了这一相对不发达的研究领域的进展。我认为血管对血管壁伸展或腔内压力增加的肌性收缩反应是一种细胞/细胞内和钙信号；3)收缩元件的跨桥特性。我本可以展示以下有趣的结果，这将为未来的研究进展提供保证。我发现，膜流动性强烈影响机械感受器部位的敏感性，而阿米洛利敏感成分，可能是牵张激活的通道，可能在肌源性活动的发生中发挥作用。此外，生物力学刺激增加了磷脂酶C的活性，产生了三磷酸肌醇，并增加了胞内Ca~(2+)浓度，这表明与这种酶结合的GTP结合蛋白在机械刺激向细胞信号的转化中可能发挥了作用。此外，还发现佛波酯的收缩和高K~(++)去极化刺激之间的生肌活动具有跨桥循环的特性。这些结果表明，血管对生物力学刺激的反应是通过一种新的细胞信号机制来调节的。