Pathogenicity of Pneumonia uirus of mice(PVM) in immunodeficient animals

小鼠肺炎（PVM）在免疫缺陷动物中的致病性

• 批准号: 04680044
• 负责人: SATO Hiroshi
• 金额: $ 1.47万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680044/
• 关键词: Uirus; Laboratory animal; Pneumonia; Mouse; Rat; Sero-epidemiological method; PVM; HVJ; Penumoria; mouse; Sero-epidenriological method; ウイルス; 実験動物; 肺炎; マウス; ラット; 免疫不全

[1] INDIRECT FLUORESCENT ANTIBODY METHOD WAS APPLIED FOR A DETECTION OF PNEUMONIA VIRUS OF MOUSE(PVM)ANTIBODY IN COLONIES OF LABORATOREY ANIMALS IN JAPAN.THE RESULTS SHOWED THAT THE ANTIBODY EXIST IN SPF RATS WITH HIGH INCIDENCE.THE ANTIBODY WAS ALSO DETECTED IN OF RABBITS.HOWEVER,THE ANTIBODY WAS NOT DEMONSTRATED IN THE SERA OF MOUSE.THESE RESULTS SUGGESTED THAT PVM INFECTION IS PRESENT IN LABORATORY RATS IN JAPAN AS WELL AS WESTERN COUNTRIES,AND POINTED OUT THE IMPORTANCE OF MICROBIOLOGICAL MONITORING FOR PVM IN FACILITIES FOR ANIMAL EXPERIMENTATION AND ANIMAL BREEDERS.[2] A TRIAL OF VIRUS ISOLATION WAS RESULTED IN POSITIVE.THE CHARACTERISTICS OF NEW ISOLATES (31N,14-3)WERE THE SAME AS THE No.15 ; STANDARD-TYPE OF PVM.THE 31N WAS INFECTED TO MICE AND RATS.AS A RESULT,31N INDUCED THE CONSOLIDATION IN LUNG.ALSO,31N INDUCED INTERSTITIAL PNEUMONIA IN MICE AND RATS.THE SEVERITY WAS HEAVIER IN MICE THAN RATS.AS THE RESULTS,THESE HISTO-PATHOLOGICAL FINDINGS IN RATS SUGGESTED THAT RESULTS WERE VERY IMPORTANT TO INVESTIGATOR OF ANIMAL EXPERIMENTATION AND ADMINISTRATOR OF ANIMAL FACILITY.

[1] 采用间接荧光抗体法对日本实验动物群体中的小鼠肺炎病毒（PVM）抗体进行检测，结果表明该抗体存在于SPF级大鼠中，且发病率较高。在兔子中检测到。然而，在小鼠血清中未显示出该抗体。这些结果表明 PVM 感染存在于 日本以及西方国家的实验室老鼠，并指出了微生物监测对动物实验和动物饲养设施中 PVM 的重要性。 [2]病毒分离试验结果呈阳性，新分离株(31N，14-3)特征与15号相同；标准型PVM。31N感染小鼠和大鼠，31N引起肺实变。同时，31N引起小鼠和大鼠间质性肺炎。小鼠的严重程度较大鼠重。因此，这些组织病理学结果大鼠研究结果表明，结果对于动物实验研究者和动物设施管理者非常重要。

项目成果

Hironori Miyata: "Serological evidence of Pneumonia virus of mouse (PVM) infection in laloratory rats" Experimental Animal. 42. 371-376 (1993)

Hironori Miyata：“实验动物中小鼠肺炎病毒（PVM）感染的血清学证据”。

Watanabe: "HVJ infection in alveolar macrophages of varisus laboratory animals." Exp.Animals. 39. 249-254 (1990)

Watanabe：“varisus 实验动物肺泡巨噬细胞中的 HVJ 感染。”

Hironori Miyata: "Serological eaidence of Pneumonia Virus of Mouse(PVM)infection in laboratory rats" Experimental Animal. 42. 371-376 (1993)

Hironori Miyata：“实验室大鼠中小鼠肺炎病毒（PVM）感染的血清学证据”实验动物。

Hironori Miyata: "New isolates of Pneumonia Virus of Mouse(PVM)from Japanese colovies and their Characterigation" Experimental Animal. in preso.

Hironori Miyata：“来自日本菌落的小鼠肺炎病毒（PVM）新分离株及其特征”实验动物。

Hironori Miyata: "Serological evidence of Pneumonia Virus of Mouse(PVM) infection in laboratory rats" Exp.Animals. 42. 371-376 (1993)

Hironori Miyata：“实验室大鼠中小鼠肺炎病毒（PVM）感染的血清学证据”Exp.Animals。