Inverse confocal microscope with light sheett and live-cell imaging function

具有光片和活细胞成像功能的反共焦显微镜

• 批准号: 442009976
• 金额: --
• 依托单位: Ruhr-Universität Bochum
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/442009976?language=en
• 关键词: Inverse; confocal; microscope; light; sheett

A deeper understanding of the mechanisms of processes affecting neurodegeneration and axonal regeneration is essential for the development of novel treatments for the repair of the damaged nervous system. The principal applicant examines different aspects of these processes in specialized cell culture models and animal models at both morphological-anatomical and sub-cellular molecular levels. The modularity of the device applied for offers the possibility of investigating the molecular mechanisms of axonal growth in high temporal and spatial resolution using live-cell-imaging and Lightning technology. Also, light-sheet technology can be used to three-dimensionally reconstruct the course of regenerated axons in whole tissue blocks after tissue clearing and to analyze parameters such as pathfinding and resynaptogenesis. These aspects are essential for the current and future research orientation of the principal applicant, as they are now used by default in many laboratories worldwide. Also, live-cell-imaging and light-sheet technology offer many opportunities for all other applicants, too, mainly as there is currently no device with the appropriate equipment available in Bochum.

深入了解影响神经变性和轴突再生的过程的机制对于开发用于修复受损神经系统的新治疗方法至关重要。主要申请人在形态解剖学和亚细胞分子水平上在专门的细胞培养模型和动物模型中检查这些过程的不同方面。所应用的设备的模块化提供了使用活细胞成像和Lightning技术以高时间和空间分辨率研究轴突生长的分子机制的可能性。此外，光片技术可用于在组织清除后三维重建整个组织块中再生轴突的过程，并分析诸如寻路和再突触发生等参数。这些方面对于主申请人当前和未来的研究方向至关重要，因为它们现在在全球许多实验室中默认使用。此外，活细胞成像和光片技术也为所有其他申请人提供了许多机会，主要是因为目前在波鸿还没有配备适当设备的设备。