Inverse confocal microscope with light sheett and live-cell imaging function

深入了解影响神经退行性变和轴突再生的过程的机制，对于开发修复受损神经系统的新疗法至关重要。主要申请人在形态解剖学和亚细胞分子水平上在专门的细胞培养模型和动物模型中检查这些过程的不同方面。该设备的模块化为利用活细胞成像和闪电技术在高时间和空间分辨率下研究轴突生长的分子机制提供了可能。此外，光片技术还可以用来三维重建组织清除后整个组织块中再生轴突的过程，并分析诸如寻路和再突触发生等参数。这些方面对于主要申请者当前和未来的研究方向至关重要，因为它们现在被世界各地的许多实验室默认使用。此外，活细胞成像和光片技术也为所有其他申请者提供了许多机会，主要是因为波鸿目前还没有配备适当设备的设备。

A deeper understanding of the mechanisms of processes affecting neurodegeneration and axonal regeneration is essential for the development of novel treatments for the repair of the damaged nervous system. The principal applicant examines different aspects of these processes in specialized cell culture models and animal models at both morphological-anatomical and sub-cellular molecular levels. The modularity of the device applied for offers the possibility of investigating the molecular mechanisms of axonal growth in high temporal and spatial resolution using live-cell-imaging and Lightning technology. Also, light-sheet technology can be used to three-dimensionally reconstruct the course of regenerated axons in whole tissue blocks after tissue clearing and to analyze parameters such as pathfinding and resynaptogenesis. These aspects are essential for the current and future research orientation of the principal applicant, as they are now used by default in many laboratories worldwide. Also, live-cell-imaging and light-sheet technology offer many opportunities for all other applicants, too, mainly as there is currently no device with the appropriate equipment available in Bochum.