Disturibution and function of autoregulator jin streptomyces species producing antibiotics

产抗生素金链霉菌属自动调节因子的分布和功能

• 批准号: 04680189
• 负责人: NIHIRA Takuya
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680189/
• 关键词: Streptomyces; vbrA; secondary metabolism

The presence of the vbrA gene encoding the transcriptional antiterminator NusG equvalent protein of Streptomyces virginiae was tested for in 73 Soteptomyces species by Southern hybridization. Fifty-five strains (75%) including S.griseus, S.lividans TK-21 and S.coelicolor A3(2) showed clear hybridization signals, indicating wide distribution of vbrA or vbrA homologs in Streptomyces species. With hybridization patterns against 3 different probes, i.e., probes covering vbrA alone, the downstream gene rplK alone, and both vbrA-rplK, the 55 strains were classified into 4 groups. In the group I, II and III (total 50 strains) vbrA was found to be adjcent to rplK, indicating that the gene arrangement vbrA-rplK is common in Streptomyces and that these gene may constitute a part of gene cluster encoding several components of the transcriptional and translational apparatus, as in Escherichia coli.

用Southern杂交法检测了73种Sotetelae菌株中编码转录抗终止子NusG等价蛋白的vbrA基因的存在。其中灰色链霉菌、变铅青链霉菌TK-21和天蓝色链霉菌A3（2）等55株（75%）显示出清晰的杂交信号，表明vbrA或vbrA同源物在链霉菌中广泛分布。利用针对3种不同探针的杂交模式，即，探针覆盖单独的vbrA、单独的下游基因rplK、以及vbrA-rplK，将55个菌株分为4组。在Ⅰ、Ⅱ和Ⅲ组（共50株）中，vbrA与rplK相邻，表明vbrA-rplK基因排列在链霉菌中很常见，这些基因可能构成了编码转录和翻译装置几种成分的基因簇的一部分，如在大肠杆菌中。

项目成果

Puttikhunt,C.et al: "Distribution in the genus Streptomyces of a homolog to nusG,a gene encoding a transcriptional antiterminator" FEMS Microbiology letters. 10. 243-248 (1993)

Puttikhunt,C.等人：“nusG 同源物在链霉菌属中的分布，nusG 是编码转录抗终止子的基因”FEMS 微生物学信件。

Chunya Puttikhunt, Susumu Okamoto, Takashi Nakamura, Takuya Nihira, Yasuhiro Yamada: "Distribution in the genus Streptomyces of a homolog to nusG, a gene encoding a transcriptional antiterminator" FEMS Microbiol. Lett.110. 143-248 (1993)

Chunya Puttikhunt、Susumu Okamoto、Takashi Nakamura、Takuy​​a Nihira、Yasuhiro Yamada：“nusG 同源物在链霉菌属中的分布，nusG 是编码转录抗终止子的基因”FEMS Microbiol。