Disturibution and function of autoregulator jin streptomyces species producing antibiotics

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The presence of the vbrA gene encoding the transcriptional antiterminator NusG equvalent protein of Streptomyces virginiae was tested for in 73 Soteptomyces species by Southern hybridization. Fifty-five strains (75%) including S.griseus, S.lividans TK-21 and S.coelicolor A3(2) showed clear hybridization signals, indicating wide distribution of vbrA or vbrA homologs in Streptomyces species. With hybridization patterns against 3 different probes, i.e., probes covering vbrA alone, the downstream gene rplK alone, and both vbrA-rplK, the 55 strains were classified into 4 groups. In the group I, II and III (total 50 strains) vbrA was found to be adjcent to rplK, indicating that the gene arrangement vbrA-rplK is common in Streptomyces and that these gene may constitute a part of gene cluster encoding several components of the transcriptional and translational apparatus, as in Escherichia coli.