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Distribution and function of novel inducing factor for secondary metabolism

新型次生代谢诱导因子的分布与功能

基本信息

批准号：
02680158
负责人：
NIHIRA Takuya
金额：
$ 1.34万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02680158/
关键词：
Virginiae Butanolide Blue Pigment Inducing Factor Streptomyces antibiotics distribution Secondary Metabolism

项目摘要

1. To know the influence of IM-2 on Streptomyces sp. FRI-5, IM-2 was added at 5-h and cell growth, blue-pigment production and antibiotic production were measured. In the absence of IM-2, cell growth continued until 12-h cultivation, and no production of blue pigment was observed until 32-h cultivation. In clear contrast to it, IM-2 addition terminated cell growth immediately, and blue-pigment production was observed after 1 h of IM-2 addition. Antibiotic production was measured by B. subtilis at as test strain by cup method. In the absence and presence of IM-2, profile of clear zone was different, suggesting the production of different antibiotics.2. An antibiotic produced in the absence of IM-2 was purified from 5-liter of culture broth after 4 purification steps : yield 13.5 mg. By NMR, IR and MS analyses, the antibiotic was identified as D-cycloserine, indicating that Streptomyces sp. FRI-5 can produce D-cycloserine in the absence of IM-2.3. Antibiotics produced in the presence of IM-2 were purified from 25-liter of culture broth after 6 purification steps. Two antibiotics (C, D) and three relating compounds (compound A1, A2 and B) were isolate : yield 2.7, 3.0, 1.3, 1.4 and 2.4 mg for compound C, D, A1, A2 and B, respectively, By NMR, IR and MS analyses, antibiotic C and D were identified as showdomycin and minimycin, respectively. Similarly, compound A1, A2 and B were alpha-showdomycin, alpha-minimycin and 5-alpha-arabinofuranosyl-1, 3-oxazin-2, 4-dione. Therefore, Streptomyces sp. FRI-5 can be concluded to produce several nucleoside antibiotics and relating compounds in the presence of IM-2.4. Production profiles for those antibiotics were measured in the absence and presence of IM-2. In the absence of IM-2, D-cycloserine was produced and no nucleoside antibiotics were detected, but the addition of IM-2 completely suppressed production of D-cycloserine and instead nucleoside antibiotics were produced.

1.了解IM-2对链霉菌的影响。在5h加入FRI-5、IM-2，测定细胞生长、蓝色素产生和抗生素产生。在没有IM-2的情况下，细胞生长一直持续到12h，直到32h才观察到蓝色素的产生。与之形成鲜明对比的是，IM-2的加入立即终止了细胞的生长，并在加入IM-2 1h后观察到蓝色素的产生。以枯草芽孢杆菌为试验菌株，采用杯法测定抗生素产量。在无IM-2和有IM-2存在的情况下，透明带的分布不同，表明产生了不同的抗生素。从5升发酵液中分离纯化出一种在无IM-2条件下产生的抗生素，经过4步纯化，得率为13.5 mg。经核磁共振、红外光谱和质谱分析，确定该抗生素为D-环丝氨酸，表明该抗生素为链霉菌属。在没有IM-2.3的情况下，FrI-5可以产生D-环丝氨酸。在IM-2存在下产生的抗生素经过6步纯化后，从25L的发酵液中得到纯化。分离得到2个抗生素(C、D)和3个相关化合物(化合物A1、A2、B)，化合物C、D、A1、A2和B的产率分别为2.7、3.0、1.3、1.4和2.4 mg，经核磁共振、红外光谱和MS分析，抗生素C和D分别为秀多霉素和极小霉素。类似地，化合物A1、A2和B为α-秀多霉素、α-极小霉素和5-α-阿拉伯呋喃糖基-1，3-恶嗪-2，4-二酮。因此，链霉菌(Streptomyces sp.)在IM-2.4存在下，FRI-5可以得出结论，产生几种核苷类抗生素和相关化合物。在没有IM-2和存在IM-2的情况下，测量这些抗生素的生产概况。在没有IM-2的情况下，产生D-环丝氨酸，没有检测到核苷类抗生素，但IM-2的加入完全抑制了D-环丝氨酸的产生，而产生了核苷类抗生素。