Macrocyclic antibiotics production-platform through biosynthetic engineering

生物合成工程大环抗生素生产平台

• 批准号: 21360404
• 负责人: NIHIRA Takuya
• 金额: $ 11.23万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21360404/
• 关键词: 生体触媒工学; 二次代謝; 生理活性物質; 生合成制御; 放線菌; 糸状菌; 生合成遺伝子; 転写活性化因子; 生合成遺伝子クラスター; 休眠遺伝子; クラスター特異的転写因子

Among the useful microorganisms on which whole genome information is available, as the host having high potential for the production of bioactive compounds, Streptomyces avermitilis for prokaryotes and As pergillus oryzae for eukaryotes were developed as the basic expression hosts. A novel Streptomyces hormone controlling secondary metabolites in S. avermitilis was isolated and its structure was elucidated for the first time in the world, together with finding many regulatory genes and identification of their functions. With A. oryzae, new expression host harboring a novel gene upregulating secondary metabolism was developed, and system to correlate biosynthetic gene cluster with products was successfully developed.

在可获得全基因组信息的有用微生物中，作为具有较高生物活性化合物生产潜力的宿主，原核生物的阿维链霉菌（Streptomyces avermitilis）和真核生物的米曲霉（as pergillus oryzae）被开发为基本表达宿主。分离到一种新的链霉菌激素，并在国际上首次阐明了其结构，发现了许多调控基因并鉴定了它们的功能。以m.o ryzae为研究对象，开发了含有上调次生代谢新基因的新表达宿主，成功构建了生物合成基因簇与产物的关联体系。

项目成果

抗生物質バージニアマイシンSの基質供給遺伝子の機能解析

抗生素维吉尼亚霉素 S 底物供应基因的功能分析

• 发表时间: 2011
• 作者: NINGSIH F;木谷茂;福島絵里子;仁平卓也
• 通讯作者: 仁平卓也

タイ王国由来薬用植物に内生する放線菌からのStreptotenol C新規誘導体の単離

从泰王国药用植物内源放线菌中分离出新的链烯醇 C 衍生物

• 发表时间: 2010
• 作者: Ratama Daduang;木谷茂;Su-Jin Kang;Arinthip Thamchaipenet;福田隆雄;五十嵐康弘;仁平卓也
• 通讯作者: 仁平卓也

Control of secondary metabolism by farX, which is involved in the γ-butyrolactone biosynthesis of Streptomyces lavendulae FRI-5

• DOI: 10.1007/s00203-010-0550-3
• 发表时间: 2010-03-01
• 期刊: ARCHIVES OF MICROBIOLOGY
• 影响因子: 2.8
• 作者: Kitani, Shigeru;Doi, Masashi;Nihira, Takuya
• 通讯作者: Nihira, Takuya

Genome Sequence of Kitasatospora setae NBRC 14216T: An Evolutionary Snapshot of the Family Streptomycetaceae

• DOI: 10.1093/dnares/dsq026
• 发表时间: 2010-12-31
• 期刊: DNA RESEARCH
• 影响因子: 4.1
• 作者: Ichikawa, Natsuko;Oguchi, Akio;Fujita, Nobuyuki
• 通讯作者: Fujita, Nobuyuki

Utilization of entomopathogenic fungi for the control of plant pathogenic oomycetes

利用昆虫病原真菌控制植物病原卵菌

• 发表时间: 2009
• 作者: Sastia Prama Putri;Hiroshi Kinoshita;Fumio Ihara;Yasuhiro Igarashi;Takuya Nihira
• 通讯作者: Takuya Nihira