Macrocyclic antibiotics production-platform through biosynthetic engineering

在可获得全基因组信息的有用微生物中，阿维链霉菌(Streptomyces Avermitilis)和米曲霉(Pergillusoryzae)是具有生产生物活性物质的高潜力宿主，它们分别用于原核生物和真核生物。从阿维链霉菌中分离到一种新的激素控制次生代谢物，其结构在世界上尚属首次，发现了多种调控基因，并对其功能进行了鉴定。利用米曲霉，开发了含有上调次生代谢的新基因的新表达宿主，并成功地建立了生物合成基因簇与产物的关联系统。

Among the useful microorganisms on which whole genome information is available, as the host having high potential for the production of bioactive compounds, Streptomyces avermitilis for prokaryotes and As pergillus oryzae for eukaryotes were developed as the basic expression hosts. A novel Streptomyces hormone controlling secondary metabolites in S. avermitilis was isolated and its structure was elucidated for the first time in the world, together with finding many regulatory genes and identification of their functions. With A. oryzae, new expression host harboring a novel gene upregulating secondary metabolism was developed, and system to correlate biosynthetic gene cluster with products was successfully developed.